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Table of Contents
Vol. 2, No. 6, 2002
Issue release date: 2002
Section title: Original Paper
Pancreatology 2002;2:519–527
(DOI:10.1159/000066094)

Expression of Drug-Metabolizing Enzymes in the Pancreas of Hamster, Mouse, and Rat, Responding Differently to the Pancreatic Carcinogenicity of BOP

Ulrich A.B. · Standop J. · Schmied B.M. · Schneider M.B. · Lawson T.A. · Pour P.M.
aUNMC Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebr., USA; bDepartment of Visceral and Transplantation Surgery, University of Heidelberg, and cDepartment of Surgery, Rheinische Friedrich-Wilhelms-University, Bonn, Germany; dDepartment of Visceral and Transplantation Surgery, Inselspital, Bern, Switzerland; Departments of ePharmaceutical Sciences and fPathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebr., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/3/2001
Accepted: 6/11/2002
Published online: 11/28/2002

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1424-3903 (Print)
eISSN: 1424-3911 (Online)

For additional information: http://www.karger.com/PAN

Abstract

Background/Methods: N-nitroso-bis(2-oxopropyl)amine (BOP) induces pancreatic ductal adenocarcinoma in Syrian golden hamsters, but not in rats or mice. To examine whether this difference is due to the diversity in the presence and distribution of enzymes involved in the metabolism of BOP, the cellular expression of nine cytochrome P-450 isozymes (CYP1A1, CYP1A2, CYP2B6, CYP2C8,9,19, CYP2D1, CYP2E1, CYP3A1, CYP3A2, and CYP3A4) and of three glutathione S-transferase isozymes (GST-π, GST-α, and GST-µ) was investigated in the pancreas of hamsters, rats, and mice by immunohistochemistry. Results: We found a wide species variation in the presence and cellular localization of the enzymes and a lack of several enzymes, including GST-α in islets, CYP2B6, CYP2C8,9,19, CYP3A1 in acinar cells, and CYP3A4 in ductal cells, in the rat as compared with hamster and mouse. Conclusion: Although the results could not clarify the reasons for the species differences in the pancreatic carcinogenicity of BOP, the presence of most of the cytochrome P-450 isozymes in pancreatic islets of all three species highlights the important role of the islets in drug metabolism.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/3/2001
Accepted: 6/11/2002
Published online: 11/28/2002

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1424-3903 (Print)
eISSN: 1424-3911 (Online)

For additional information: http://www.karger.com/PAN


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

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