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Vol. 49, No. 1, 2003
Issue release date: January–February 2003
Gerontology 2003;49:27–32
(DOI:10.1159/000066505)

Effect of Age in Rats following Middle Cerebral Artery Occlusion

Wang R.-Y. · Wang P.S.-G. · Yang Y.-R.
aInstitute and Faculty of Physical Therapy and bDepartment of Physiology, National Yang-Ming University, Shih-Pai, Taipei, Taiwan/ROC

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Abstract

Background: Despite the impressive increased understanding of the ischemic brain damage in general, the study of effects on different age groups, young versus old, using comparable ischemic insults is clearly lacking. Objectives: To investigate the mortality rate and neurological outcome among young and old rats, through a model of cerebral ischemia by middle cerebral artery occlusion (MCAO). Methods: Twenty-three old (22–24 months of age) and 16 young (3–4 months of age) male rats underwent a MCAO procedure for 60 min. Surviving rats were randomly assigned to the 24-hour or 28-day resting group. The mortality rate and neurological outcome in different recovery time periods were determined for comparison between the young and old rats. Results: The overall mortality rate in old rats (43.5%) was significantly higher than that of the young rats (6.3%) (p = 0.01). The infarct volume for the 24-hour post-MCAO was 181.86 ± 11.87 mm3 for the young rats, and 204.64 ± 27.18 mm3 for the old rats. For the 28-day post-MCAO, the value was 91.16 ± 3.59 mm3 for the young rats, and 103.38 ± 26.43 mm3 for the old rats. A significant reduction in infarct volume is noted in both young (p < 0.01) and old (p < 0.05) rats after 28 days of recovery compared to that after 24 h of recovery. There was no meaningful difference in infarct volume between the young and old rats measured at 24 h or 28 days after the ischemic procedure. The right volume was larger than the left volume at 24 h post-MCAO for both the young and the old rats, whereas the quotient approached unity for the young rats at 28 days post-MCAO. For the old rats, the quotient was negative at 28 days post-MCAO, representing the ipsilateral hemisphere was smaller than the contralateral hemisphere. Conclusion: The mortality risk to ischemic damage is greater for old rats. If an old rat survives the high-risk mortality in a short period after the MCAO procedure, the recovery would be of no difference to that of a young rat.



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References

  1. Baughman VL, Hoffman WE, Thomas C, Miletich DJ, Albrecht RF: Neurologic outcome in aged rats after incomplete cerebral ischemia. Anesth Analg 1988;67:677–682.
  2. Naritomi H, Meyler JS, Sakai F, Yamaguchi F, Shaw T: Effects of advancing age on regional cerebral blood flow: Studies in normal subjects and subjects with risk factors for atherothrombotic stroke. Arch Neurol 1979;36:410–416.
  3. Sokoloff L: Cerebral circulation and metabolism in the aged; in Gershon S, Roskin A (eds): Aging. New York, Raven Press, 1975, pp 45–54.
  4. Birren JE, Butler R, Greenhouse SW: Human Aging: A Biological and Behavioral Study. PHS Pub No 986. Washington, US Government Printing Office, 1963.
  5. Naber D, Dahnke HG: Protein and nucleic acid content in the aging human brain. Neuropath Appl Neurobiol 1979;5:17–24.
  6. Meier-Ruge W, Iwangoff P, Reichlmeier K, Sandoz P: Neurochemical findings in the aging brain. Adv Biochem Psychopharmacol 1980;23:23–38.
  7. Pentland B, Jones PA, Roy CW, Miller JD: Head injury in the elderly. Age Aging 1986;15:193–202.
  8. Hoyer S: Ischemia in the aged brain. Gerontology 1987;33:203–206.
  9. Lin TN, He YY, Wu G, Khan M, Hsu CY: Effect of brain edema on infarct volume in a focal cerebral ischemia model in rats. Stroke 1993;24:117–121.
  10. Wang RY, Yang YR, Yu SM: Protective effects of treadmill training on infarction in rats. Brain Res 2001;922:140–143.
  11. Swanson RA, Morton MT; Tsao-Wu G, Savalos RA, Davidson C, Sharp FR: A semiautomated method for measuring brain infarct volume. J Cereb Blood Flow Metab 1990;10:290–293.
  12. Aspey BS, Cohen S, Patel Y, Terruli M, Harrison MJ: Middle cerebral artery occlusion in the rat: Consistent protocol for a model of stroke. Neuropathol Appl Neurobiol 1998;24:487–497.
  13. Strandgaard S: Autoregulation of cerebral blood flow in hypertensive patients: The modifying influence of prolonged antihypertensive treatment on the tolerance to acute, drug-induced hypotension. Circulation 1976;53:720–727.
  14. Frolkis VV, Bezrukov VV, Duplenko YK, Shchegoleva IV, Shevtchuk VG, Verkhratsky NS: Acetylcholine metabolism and cholinergic regulation of functions in aging. Gerontologia 1973;19:45–57.
  15. Hamm RJ, Jenkins LW, Lyeth BG, White-Gbadebo DM, Hayes RL: The effect of age on outcome following traumatic brain injury in rats. J Neurosurg 1991;75:916–921.
  16. Calderini G, Bellini F, Consolazione A, Dal Toso R, Milan F, Toffano G: Reparative processes in aged brain. Gerontology 1987;33:227–233.
  17. Duverger D, MacKenzie ET: The quantification of cerebral infarction following focal ischemia in the rat: Influence of strain, arterial pressure, blood glucose concentration, and age. J Cereb Blood Flow Metab 1988;8:449–461.
  18. Krupinski J, Kaluza J, Kumar P, Kumar S, Wang JM: Role of angiogenesis in patients with cerebral ischemic stroke. Stroke 1994;25:1794–1798.
  19. Creasey H, Rapoport SI: The aging human brain. Ann Neurol 1985;17:2–10.
  20. Wexler BC: Carotid artery ligation-induced cerebral ischemia in old, male, Sprague-Dawley rats. Age 1979;2:39–43.
  21. Persson L, Hardemark HG, Bolander HG, Hillered L, Olsson Y: Neurologic and neuropathologic outcome after middle cerebral artery occlusion in rats. Stroke 1989;20:641–645.
  22. Kharlamov A, Kharlamov E, Armstrong DM: Age-dependent increase in infarct volume following photochemically induced cerebral infarction; putative role of astroglia. J Gerontol Biol Sci 2000;55A:B135–B141.


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