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Vol. 129, No. 3, 2002
Issue release date: November 2002
Int Arch Allergy Immunol 2002;129:181–188
(DOI:10.1159/000066780)

Immunobiology of Fungal Allergens

Kurup V.P. · Shen H.-D. · Vijay H.
aAllergy-Immunology Division, Medical College of Wisconsin, and bResearch Service, VA Medical Center, Milwaukee, Wisc., USA; cDepartment of Medical Research, Veterans General Hospital, Taipei, Taiwan/ROC and dEnvironmental Health Directorate, Health Canada, Ottawa, Ont., Canada

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Abstract

In recent years, considerable attention has been paid in obtaining purified relevant allergens from fungi associated with allergy. Using molecular biology techniques, a number of mold allergens have been obtained by cloning the genes encoding the allergens. Currently, about 70 fungal allergens have been approved by the International Allergen Nomenclature Committee. In this review, we have presented major allergens from Aspergillus, Penicillium, Cladosporium, and Alternaria and discussed their immunochemical characteristics and their role in the diagnosis of allergy and possible usefulness in understanding the pathogenesis of the disease. The structure-function properties and the potential role of these recombinant allergens in the immunomodulatory therapy also are presented.



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References

  1. Kurup VP, Fink JN: Fungal allergy; in Murphy JW, Friedman H, Bendinelli M (eds): Fungal Infections and Immune Responses. New York, Plenum Press, 1993, pp 393–404.
  2. Burge HA: Airborne-allergenic fungi. Immunol Allergy Clin North Am 1989;9:307–319.
  3. Greenberger PA: Allergic bronchopulmonary aspergillosis and fungoses. Clin Chest Med 1988;9:599–608.
  4. Katzenstein AA, Sale SR, Greenberger PA: Allergic Aspergillus sinusitis: A newly recognized form of sinusitis. J Allergy Clin Immunol 1983;72:89–98.
  5. Kurup VP: Hypersensitivity pneumonitis due to sensitization with thermophilic actinomycetes; in Solomon WR (ed): Airborne Allergens. Immunology and Allergy Clinics of North America. Philadelphia, Saunders, 1989, pp 285–306.
  6. Vijay HM, Thaker AJ, Banerjee B, Kurup VP: Mold allergens; in Lockey RF, Bukantz SC (eds): Allergens and Allergen Immunotherapy. New York, Dekker, 1998, pp 133–154.
  7. Portnoy J, Chapman J, Burge H, Muielenberg M, Solomon W: Epicoccum allergy. Skin reaction patterns and spore/mycelium disparities recognized by IgG and IgE ELISA inhibition. Ann Allgery 1987;59:39–43.
  8. Wuethrich B: Epidemiology of allergic diseases: Are they really on the increase. Int Arch Allergy Appl Immunol 1989;90:3–10.

    External Resources

  9. Kurup VP, Shen HD, Banerjee B: Respiratory fungal allergy. Microbes Infect 2000;2:1101–1110.
  10. Kurup VP: Immunopathogenesis of fungal induced asthma and allergy. Proceedings of International Symposium on Recent Advances in Molecular Biology, Allergy, Immunology, Baroda, India, Sept 3–5, 2000, pp 81–94.
  11. Gravesen S: Microfungal contamination of damp buildings: Biological aspects; in Johanning E (ed): Bioaerosols, Fungi and Mycotoxins. New York, Eastern New York Occupational and Environmental Health Center, 1999, pp 505–511.
  12. Kozak PP Jr, Gallup J, Cummins LH, Gilmman SA: Factors of importance in determining the prevalence of indoor molds. Ann Allergy 1979;43:88–94.
  13. Burge HA: Fungus allergens. Clin Rev Allergy 1985;3:319–329.
  14. Licorish K, Novey HS, Kozak P, Fairshter RD, Wilson AF: Role of Alternaria and Penicillium spores in the pathogenesis of asthma. J Allergy Clin Immunol 1985;76:819–825.
  15. Bierman WC, Van Arsdel PP Jr: Clinical evaluation of the patient with allergic and immunologic disease; in Lockey RF, Bukantz SC (eds): Principles of Immunology and Allergy. Philadelphia, Saunders, 1987, pp 1–26.
  16. Blumenthal MN, Rosenberg A: Definitions of an allergen (immunobiology); in Lockey RF, Bukantz SC (eds): Allergens and Allergen Immunotherapy. New York, Dekker, 1999, pp 39–51.
  17. Scheiner O, Kraft D: Basic and practical aspects of recombinant allergens (review). Allergy 1995;50:384–391.
  18. Chapman MD, Smith AM, Vailes LD, Arruda LK, Dhanaraj V, Pomes A: Recombinant allergens for diagnosis and therapy of allergic disease. J Allergy Clin Immunol 2000;106:409–418.
  19. Valenta R, Steinberger P, Duchene M, Kraft D: Immunological and structural similarities among allergens: Prerequisite for a specific and component-based therapy of allergy. Immunol Cell Biol 1996;74:187–194.
  20. Wilson IA, Stanfield RL: Antibody-antigen interactions: New structures and new conformational changes. Curr Opin Struct Biol 1994;4:857–867.
  21. Stewart GA, Thompson PJ: The biochemistry of common aeroallergens. Clin Exp Allergy 1996;26:1020–1044.

    External Resources

  22. Valenta R, Flicker S, Eibensteiner PB, Steinberger P, Laffer S, Doleck C, Kraft D: Recombinant allergen-specific antibody fragments: Tools for diagnosis, prevention and therapy of type I allergy. Biol Chem 1997;378:745–749.
  23. Official list of allergens: IUIS Allergen Nomenclature Subcommittee, http://www.allergen. org.
  24. Achatz G, Oberkofler H, Lechenauer E, Simon B, Unger A, Kandler D, Ebner C, Prillinger H, Kraft D, Breitenbach M: Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum. Mol Immunol 1995;32:213–227.

    External Resources

  25. DeVouge MW, Thaker AJ, Curran IHA, Zhang L, Muradia G, Rode H, Vijay HM: Isolation and expression of cDNA clone encoding Alternaria alternata Alt a 1 subunit. Int Arch Allergy Immunol 1996;111:385–395.
  26. DeVouge MW, Thaker AJ, Zhang L, Muradia G, Rode H, Vijay HM: Molecular cloning of IgE binding fragments of Alternaria alternata allergens. Int Arch Allergy Immunol 1998;116:261–268.
  27. Kendrick B: The history of conidial fungi; in Cole GT, Kendrick B (eds): Biology of Conidial Fungi. New York, Academic Press, 1981.
  28. Becker AB, Muradia G, Vijay HM: Immunoreactive Alternaria allergens in house dust in the absence of environmental mold (abstract). J Allergy Clin Immunol 1996;97:220.
  29. Garrett MH, Hooper BM, Cole FM, Hooper MA: Airborne fungal spores in 80 homes in Latrobe Valley, Australia: Levels, seasonality and indoor-outdoor relationship. Aerobiologia (Ireland) 1997;13:121–126.
  30. Curran IHA, Young NM, Burton M, Vijay HM: Purification and characterization of Alt a 29 from Alternaria alternata. Int Arch Allergy Immunol 1993;102:267–275.
  31. Matthiesen F, Olsen M, Lowenstein H: Purification and partial sequenization of the major allergen of Alternaria alternata Alt a 1 (abstract). J Allergy Clin Immunol 1992;89:241.
  32. Akurst L, Borch SM: Partial purification and characterization of two Cladosporium herbarum allergens. Int Arch Allergy Appl Immunol 1979;60:68–79.
  33. Sward-Nordmo M, Wold JK, Paulsen BS, Aukrust L: Purification and partial characterization of the allergen Ag54 from Cladosporium herbarum. Int Arch Allergy Appl Immunol 1985;78:249–255.
  34. Sward-Nordmo M, Paulsen BS, Wold JK: Immunological studies of glycoprotein allergen Ag-54 (Cla h II) in Cladosporium herbarum with special attention to the carbohydrate and protein moieties. Int Arch Allergy Appl Immunol 1989;90:155–161.
  35. Zhang L, Muradia G, Curran IHA, Rode H, Vijay HM: A cDNA clone coding for a novel allergen, Cla h III, of Cladosporium herbarum identified as a ribosomal P2 protein. J Immunol 1995;154:710–716.
  36. Breitenbach M, Simon B, Probst G, Oberkofler H, Ferreira F, Briza P, Achatz G, Unger A, Ebner C, Kraft D, Hirschwehr R: Enolases are highly conserved fungal allergens. Int Arch Allergy Immunol 1997;113:114–117.
  37. Kurup VP, Kumar A: Immunodiagnosis of aspergillosis. Clin Microbiol Rev 1991;4:439–459.
  38. Kurup VP, Apter A: Immunology and Allergy Clinics of North America. Philadelphia, Saunders, 1998, vol 18.
  39. Moser M, Crameri R, Menz G, Schneider T, Dudler T, Virchow C, Gmachl M, Blaser K, Surer M: Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity. J Immunol 1992;149:454–460.
  40. Kurup VP, Banerjee B, Hemmann S, Greenberger PA, Blaser K, Crameri R: Selected recombinant Aspergillus fumigatus allergens bind specifically to IgE in ABPA. Clin Exp Allergy 2000;30:988–993.
  41. Ramachandran H, Jayaraman V, Banerjee B, Greenberger PA, Kelly KJ, Fink JN, Kurup VP: IgE binding conformational epitopes of Asp f 3, a major allergen of Aspergillus fumigatus. Clin Immunol 2002;103:324–333.
  42. Crameri R: Recombinant Aspergillus fumigatus allergens: From the nucleotide sequences to clinical applications. Int Arch Allergy Immunol 1998;115:99–114.
  43. Banerjee B, Kurup VP: Molecular biology of Aspergillus allergens; in Kurup VP, Apter AJ (eds): Immunology and Allergy Clinics of North America. Philadelphia, Saunders, 1998, vol 18, pp 601–618.
  44. Shen HD, Lin WL, Tam MF, Wang SR, Tsai JJ, Chou H, Han SH: Alkaline serine proteinase: A major allergen of Aspergillus oryzae and its cross-reactivity with Penicillium citrinum. Int Arch Allergy Immunol 1998;116:29–35.
  45. Shen HD, Tam MF, Chou H, Han SH: The importance of serine proteinases as aeroallergens associated with asthma. Int Arch Allergy Immunol 1999;119:259–264.
  46. Shen HD, Lin WL, Liaw SF, Tam MF, Han SH: Characterization of the 33-kilodalton major allergen of Penicillium citrinum by using MoAbs and N-terminal amino acid sequencing. Clin Exp Allergy 1997;27:79–86.

    External Resources

  47. Chou H, Lai HY, Tam MF, Chou MY, Wang SR, Han SH, Shen HD: cDNA cloning, biological and immunological characterization of the alkaline serine protease major allergen from Penicillium chrysognum. Int Arch Allergy Immunol 2002;127:15–26.
  48. Shen HD, Wang CW, Lin WL, Lai HY, Tam MF, Chou H, Wang SR, Han SH: cDNA cloning and immunological characterization of Pen o 18, the vacuolar serine protease major allergen of Penicillium oxalicum. J Lab Clin Med 2001;137:115–124.
  49. Chou H, Lin WL, Tam MF, Wang SR, Han SH, Shen HD: Alkaline serine proteinase is a major allergen of Aspergillus flavus – A prevalent airborne Aspergillus species in the Taipei area. Int Arch Allergy Immunol 1999;119:282–290.
  50. Muilenberg M, Burge H, Sweet T, Solomon W: Penicillium species in and out of doors in Topeka KS. J Allergy Clin Immunol 1990;85:247.
  51. Wie DL, Chen JH, Jong SC, Shen HD: Indoor airborne Penicillium species in Taiwan. Curr Microbiol 1993;26:137–140.
  52. Shen HD, Lin WL, Tsai JJ, Liaw SF, Han SH: Allergenic components in three different species of Penicillium: Cross-reactivity among major allergens. Clin Exp Allergy 1996;26:444–451.

    External Resources

  53. Su NY, Yu CJ, Shen HD, Pan FM, Chow LP: Pen c 1, a novel enzymic allergen protein from Penicillium citrinum. Purification, characterization, cloning and expression. Eur J Biochem 1999;261:115–123.

    External Resources

  54. Shen HD, Lin WL, Tam MF, Chou H, Wang CW, Tsai JJ, Wang SR, Han SH: Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis. Clin Exp Allergy 2001;31:295–302.

    External Resources

  55. Shen HD, Liaw SF, Lin WL, Ro LH, Yang HL, Han SH: Molecular cloning of cDNA coding for the 68 kDa allergen of Penicillium notatum using MoAbs. Clin Exp Allergy 1995;25:350–356.
  56. Shen HD, Au LC, Lin WL, Liaw SF, Tsai JJ, Han SH: Molecular cloning and expression of a Penicillium citrinum allergen with sequence homology and antigenic cross-reactivity to a hsp 70 human heat shock protein. Clin Exp Allergy 1997;27:682–690.

    External Resources

  57. Shen HD, Wang CW, Chou H, Lin WL, Tam MF, Huang MH, Kuo ML, Wang SR, Han SH: cDNA cloning and immunological characterization of a new Penicillium citrinum allergen (Pen c 3). J Allergy Clin Immunol 2000;105:827–833.
  58. Lai HY, Tam MF, Tang RB, Chou H, Chang CY, Tsai JJ, Shen HD: cDNA cloning and immunological characterization of a newly identified enolase allergen from Penicillium citrinum and Aspergillus fumigatus. Int Arch Allergy Immunol 2002;127:181–190.
  59. Horner WE, Helbling A, Salvaggio JE, Lehrer SB: Fungal allergens. Clin Microbiol Rev 1995;8:161–179.
  60. Ishiguro A, Homma M, Torii S, Tanaka K: Identification of Candida albicans antigens reactive with immunoglobulin E antibody of human sera. Infect Immun 1992;60:1550–1557.
  61. Baldo BA, Baker RS: Inhalant allergies to fungi: Reactions to bakers’ yeast (Saccharomyces cerevisiae) and identification of bakers’ yeast enolase as an important allergen. Int Arch Allergy Appl Immunol 1988;86:201–208.
  62. Woodfolk JA, Wheatley LM, Piyasena RN, Benjamin DC, Platts-Mills TAE: Trichophyton antigens associated with IgE antibodies and delayed-type hypersensitivity. Sequence homology to two families of serine proteinases. J Biol Chem 1998;273:29489–29496.
  63. Verma J, Gangal SV: Fusarium solani: Immunochemical characterization of allergens. Int Arch Allergy Immunol 1994;104:175–183.
  64. Chauhan B, Santiago L, Kirschmann DA: The association of HLA-DR alleles and T-cell activation with allergic bronchopulmonary aspergillosis. J Immunol 1997;159:4072–4076.
  65. Chauhan B, Knutsen AP, Hutcheson PS, Slavin RG, Bellone CJ: T-cell subsets, epitope mapping, and HLA-restriction in patients with allergic bronchopulmonary aspergillosis. J Clin Invest 1996;97:2324–2331.
  66. Hudeez F: Prediction of B- and T-cell epitopes; in Raynavolgyi E (ed): Synthetic Peptides in the Search for B- and T-Cell Epitopes. Austin, RG Landes, 1994, pp 19–30.
  67. Kurup VP, Banerjee B, Murali PS, Greenberger PA, Krishnan M, Hari V, Fink JN: Immunodominant peptide epitopes of allergen, Asp f 1 from the fungus Aspergillus fumigatus. Peptides 1998;19:1469–1477.
  68. Knutsen AP, Mueller KR, Levine AD, Chauhan B, Hutcheson PS, Slavin RG: Asp f 1 CD4+ T cell lines in allergic bronchopulmonary aspergillosis. J Allergy Clin Immunol 1994;92:215–221.
  69. Kurup VP, Hari V, Guo J, Murali PS, Krishnan M, Fink JN: Aspergillus fumigatus peptides differentially express Th1 and Th2 cytokines. Peptides 1996;17:183–190.
  70. Rathore VB, Johnson B, Fink JN, Kelly KJ, Greenberger PA, Kurup VP: T-cell proliferation and cytokine secretion to T-cell epitopes of Asp f 2 in ABPA patients. Clin Immunol 2001;100:228–235.
  71. Pettersson I: Methods of epitope mapping (review). Mol Biol Rep 1992;16:149–153.
  72. Banerjee B, Greenberger PA, Fink JN, Kurup VP: Conformational and linear B-cell epitopes of Asp f 2, a major allergen of Aspergillus fumigatus, bind differently to immunoglobulin E antibody in the sera of allergic bronchopulmonary aspergillosis patients. Infect Immun 1999;67:2284–2291.
  73. Vailes LD, Li Y, Bao Y, DeGroot H, Aalberse RC, Chapman MD: Fine specificity of B cell epitopes on Felis domesticus allergen (Fel d 1): Effect of reduction and alkylation or deglycosylation on Fel d 1 structure and antibody binding. J Allergy Clin Immunol 1994;93:22–33.
  74. Ikagawa S, Matsushita S, Chen YZ, Ishikawa T, Nishimura T: Single amino acid substitutions on a Japanese cedar pollen allergen (Cry j 1)-derived peptide induced alterations in human T-cell responses and T-cell receptor antagonism. J Allergy Clin Immunol 1996;97:53–64.
  75. Tang B, Banerjee B, Greenberger PA, Fink JN, Kelly KJ, Kurup VP: Antibody binding of deletion mutants of Asp f 2, the major Aspergillus fumigatus allergen. Biochem Biophys Res Comm 2000;270:1128–1135.
  76. Banerjee B, Kurup VP, Greenberger PA, Kelly KJ, Fink JN: C-terminal cysteine residues determine the IgE binding of Aspergillus fumigatus allergen Asp f 2. J Immunol 2002, in press.
  77. Brandhorst T, Yang R, Knealy WR: Heterologous expression of the cytotoxin restrictocin in Aspergillus nidulans and Aspergillus niger. Protein Expr Purif 1994;5:486–497.
  78. Flückiger S, Mittl PRE, Scappozza L, Fijten H, Folkers G, Grütter MG, Blaser K, Crameri R: Comparison of the crystal structures of the human manganese superoxide dismutase and the homologous Aspergillus fumigatus allergen at 2-Å resolution. J Immunol 2002;168:1267–1272.
  79. Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchene M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R: Microarrayed allergen molecules: Diagnostic gatekeepers for allergy treatment. 2002 http://www.fasebj.org/cgi/doi/10.1096/fj.01–0711fje


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