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Vol. 47, No. 3-4, 2003
Issue release date: May–August 2003

Food Contents and Biological Activity of 25-Hydroxyvitamin D: A Vitamin D Metabolite to Be Reckoned With?

Ovesen L. · Brot C. · Jakobsen J.
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Only a limited number of foods naturally contain vitamin D such as fish, meat and offal, and eggs, and milk and dairy products. However, all these foods in addition contain the metabolite 25-hydroxyvitamin D (25OHD). From the few systematic studies which have been performed the food contents of 25OHD in animal foods are usually low but vary. Contents are typically very low in milk and fish (<0.1 µg/100 g), somewhat higher in meat and offal (0.2–0.4 µg/100 g) and up to 1 µg/100 g in egg yolk. It has been demonstrated that 25OHD is absorbed better and faster from the diet than native vitamin D and has metabolic effects of its own in regulating cell growth and calcium metabolism. Thus, the biological activity of 25OHD is greater than that of native vitamin D. However, there is as yet no consensus on the conversion factor that should be used for 25OHD to calculate vitamin D activity. Depending on the testing system used the factor varies from 1.5 to 5. If food contents and the higher potency of 25OHD are not included in dietary intake surveys, true vitamin D intake will be underestimated.

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  1. Brown AJ, Dusso A, Slatopolsky E: Vitamin D. Am J Physiol 1999;277:F157–F175.
  2. Olson EB, DeLuca HF: 25-Hydroxycholecalciferol: Direct effect on calcium transport. Science 1969;165:405–407.
  3. Trummel CL, Raisz LG, Blunt JW, DeLuca HF: 25-Hydroxycholecalciferol: Stimulation of bone resorption in tissue culture. Science 1969;163:1450–1451.
  4. Heaney RP, Barger-Lux MJ, Dowell MS, Chen TC, Holick MF: Calcium absorptive effects of vitamin D and its major metabolites. J Clin Endocrinol Metab 1997;82:4111–4116.
  5. Bell NH, Epstein S, Shary J, Greene V, Oexman MJ, Shaw S: Evidence of a probable role for 25-hydroxyvitamin D in the regulation of human calcium metabolism. J Bone Miner Res 1988;3:489–495.
  6. Barger-Lux MJ, Heaney RP, Lanspa SJ, Healy JC, DeLuca HF: An investigation of sources of variation in calcium absorption efficiency. J Clin Endocrinol Metab 1995;80:406–411.
  7. Colodro IH, Brickman AS, Coburn JW, Osborn TW, Norman AW: Effect of 25-hydroxy-vitamin D3 on intestinal absorption of calcium in normal man and patients with renal failure. Metabolism 1978;27:745–753.
  8. Andersen NL, Fagt S, Groth M, Hartkopp HB, Møller A, Ovesen L, Warming DL: Danish Dietary Survey 1995. Levnedsmiddelstyrelsen, Publ No 235, 1996.
  9. Deharveng G, Charrondière UR, Slimani N, Southgate DAT, Riboli E: Comparison of nutrients in the food composition tables available in nine European countries participating in EPIC. Eur J Clin Nutr 1999;53:60–79.
  10. Stern PH: The D vitamins and bone. Pharmacol Rev 1980;32:47–80.
  11. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P: Vitamin D contents in edible mushrooms. J Agric Food Chem 1994;42:2449–2453.
  12. Bills CE: Vitamin D assay – Line test and chemical methods. Biol Symp 1947;12:409–434.
  13. Hollis BW, Roos BA, Draper HH, Lambert PW: Vitamin D and its metabolites in human and bovine milk. J Nutr 1981;111:1240–1248.
  14. Reeve LE, Chesney RW, DeLuca HF: Vitamin D of human milk: Identification of biologically active forms. Am J Clin Nutr 1982;36:122–126.
  15. Weisman Y, Bawnik JC, Eisenberg Z, Spirer Z: Vitamin D metabolites in human milk. J Pediatr 1982;100:745–748.
  16. Kunz C, Niesen M, Lilienfeld-Toal HV, Burmeister W: Vitamin D,25-hydroxy-vitamin D and 1,25-dihydroxy-vitamin D in cow’s milk, infant formulas and breast milk during different stages of lactation. Int J Vitam Nutr Res 1984;54:141–148.
  17. Parviainen MT, Koskinen T, Ala-Houhala M, Visakorpi JK: A method for routine estimation of vitamin D activity in human and bovine milk. Acta Vitaminol Enzymol 1984;6:211–219.
  18. Specker BL, Tsang RC, Hollis BW: Effect of race and diet on human-milk vitamin D and 25-hydroxyvitamin D. Am J Dis Child 1985;139:1134–1137.
  19. Cancela L, Le Boulch N, Miravet L: Relationship between the vitamin D content of maternal milk and the vitamin D status of nursing women and breast-fed infants. J Endocrinol 1986;110:43–50.
  20. Hollis BW, Pittard WB, Reinhardt TA: Relationships among vitamin D,25-hydroxyvitamin D, and vitamin D-binding protein concentrations in the plasma and milk of human subjects. J Clin Endocrinol Metab 1986;62:41–44.
  21. Ala-Houhala M, Koskinen T, Parviainen MT, Visakorpi JK: 25-Hydroxyvitamin D and vitamin D in human milk: Effects of supplementation and season. Am J Clin Nutr 1988;48:1057–1060.
  22. Takeuchi A, Okano T, Tsugawa N, Tasaka Y, Kobayashi T, Kodama S, Matsuo T: Effects of ergocalciferol supplementation on the concentration of vitamin D and its metabolites in human milk. J Nutr 1989;119:1639–1646.
  23. Greer FR, Hollis BW, Cripps DJ, Tsang RC: Effects of maternal ultraviolet B irradiation on vitamin D content of human milk, J Pediatr 1984;105:431–433.
  24. Reeve LE, Jorgensen NA, DeLuca HF: Vitamin D compounds in cow’s milk. J Nutr 1982;112:667–672.
  25. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P: Contents of cholecalciferol, ergocalciferol, and their 25-hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC. J Agric Food Chem 1994;43:2394–2399.
  26. McDermott CM, Beitz DC, Littledike ET, Horst RL: Effects of dietary vitamin D3 on concentrations of vitamin D and its metabolites in blood plasma and milk of dairy cows. J Dairy Sci 1985;68:1959–1967.
  27. Koshy KT, VanDerSlik AL: High-performance liquid chromatographic method for the determination of 25-hydroxycholecalciferol in chicken eggs yolks. J Agric Food Chem 1979;27:180–183.
  28. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P: Determination of 25-hydroxycholecalciferol content in egg yolk by HPLC. J Food Comp Anal 1993;6:250–255.
  29. Mattila P, Lehikoinen K, Kiiskinen T, Piironen V: Cholecalciferol and 25-hydroxycholecalciferol content of chicken egg yolk as affected by the cholecalciferol content of feed. J Agric Food Chem 1999;47:4089–4092.
  30. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P: Cholecalciferol and 25-hydroxycholecalciferol contents in fish and fish products. J Food Comp Anal 1995;8:232–243.
  31. Takeuchi A, Okano T, Kobayashi T: The existence of 25-hydroxyvitamin D3-1α-hydroxylase in the liver of carp and bastard halibut. Life Sci 1991;48:275–282.
  32. MAFF: Determination of 25-OH Vitamin D in Selected Foodstuffs. MAFF Project No 2BO32. Report No: AS50/97/04, 1997.
  33. Takeuchi A, Okano T, Sayamoto M, Sawamura S, Kobayashi T, Motosugi S, Yamakawa T, Tissue ditribution of 7-dehydrocholesterol, vitamin D3 and 25-hydroxyvitamin D3 in several species of fish. J Nutr Sci Vitaminol 1986;32:13–22.
  34. Koshy KT, VanDer Slik AL: High performance liquid chromatographic method for determination of 25-hydroxycholecalciferol in the bovine liver, kidney, and muscle. J Agric Food Chem 1977;25:1246–1249.
  35. Thompson JN, Plouffe L: Determination of cholecalciferol in meat and fat from livestock fed normal and excessive quantities of vitamin D. Food Chem 1993;46:313–318.
  36. Thompson GR, Lewis B, Booth CC: Absorption of vitamin D3-3H in control subjects and patients with intestinal malabsorption. J Clin Invest 1966;45:94–102.
  37. Blomstrand R, Forsgren L: Intestinal adsorption and esterification of vitamin D31,2-3H in man. Acta Chem Scand 1967;21:1662–1663.
  38. Nechema H, Hoff D, Harell A, Edelstein S: The intestinal absorption of vitamin D and its metabolites. J Mol Med 1977;2:413–422.
  39. Maislos M, Silver J, Fainaru M: Intestinal absorption of vitamin D sterols: Differential absorption into lymph and portal blood in the rat. Gastroenterology 1981;70:1528–1534.
  40. Sitrin MD, Pollack KL, Bolt MJG, Rosenberg IH: Comparison of vitamin D and 25-hydroxyvitamin D absorption in the rat. Am J Physiol 1982;242:G326–G332.
  41. Davies M, Mawer EB, Krawitt EL: Comparative absorption of vitamin D3 and 25-hydroxyvitamin D3 in intestinal disease. Gut 1980;21:287–292.
  42. Krawitt EL, Chastenay BF: 25-Hydroxy vitamin D absorption test in patients with gastrointestinal disorders. Calcif Tissue Int 1980;32:183–187.
  43. Sitrin MD, Bengoa JM: Intestinal absorption of cholecalciferol and 25-hydroxycholecalciferol in chronic cholestatic liver disease. Am J Clin Nutr 1987;46:1011–1015.
  44. Compston JE, Merrett AL, Hammett FG, Magill P: Comparison of the appearance of radiolabelled vitamin D3 and 25-hydroxy-vitamin D3 in the chylomicron fraction of plasma after oral administration in man. Clin Sci 1981;60:241–243.
  45. Stamp TCB, Haddad JG, Twigg CA: Comparison of oral 25-hydroxycholecalciferol, vitamin D, and ultraviolet light as determinants of circulating 25-hydroxyvitamin D. Lancet 1977;i:1341–1343.
  46. Lund J, DeLuca HF: Biologically active metabolite of vitamin D3 from bone, liver, and blood serum. J Lipid Res 1966;7:739–744.
  47. Clements MR, Davies M, Hayes ME, Hickey CD, Lumb GA, Mawer EB, Adams PH: The role of 1,25-dihydroxyvitamin D in the mechanism of acquired vitamin D deficiency. Clin Endocrinol 1992;37:17–27.
  48. Mawer EB, Backhouse J, Holman CA, Lumb GA, Stanbury SW: The distribution and storage of vitamin D and its metabolites in human tissues. Clin Sci 1972;43:413–431.
  49. Vieth R: Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety. Am J Clin Nutr 1999;69:842–856.
  50. Whyte MP, Haddad JG, Walters DD, Stamp TCB: Vitamin D bioavailability: Serum 25-hydroxyvitamin D levels in man after oral, subcutaneous, intramuscular, and intravenous vitamin A administration. J Clin Endocrinol Metab 1979;48:906–911.
  51. Shepard RM, DeLuca HF: Plasma concentrations of vitamin D3 and its metabolites in the rat as influenced by vitamin D3 or 25-hydroxyvitamin D3 intakes. Arch Biochem Biophys 1980;202:43–53.
  52. Vieth R, McCarten K, Norwich KH: Role of 25-hydroxyvitamin D3 dose in determining rat 1,25-dihydroxyvitamin D3 production. Am J Physiol 1990;258:E780–E789.
  53. Francis RM, Peacock M, Storer JH, Davies AEJ, Brown WB, Nordin BEC: Calcium malabsorption in the elderly: The effect of treatment with oral 25-hydroxyvitamin D3. Eur J Clin Invest 1983;13:391–396.
  54. Haddad JG, Walgate J: 25-Hydroxyvitamin D transport in human plasma. J Biol Chem 1976;251:4803–4809.
  55. Teegarden D, Meridith SC, Sitrin M: Isolation and characterization of a 25-hydroxyvitamin D binding protein from rat enterocyte cytosol. J Nutr Biochem 1997;8:195–200.
  56. Teegarden D, Nickel KP, Shi L: Characterization of 25-hydroxyvitamin D binding protein from intestinal cells. Biochem Biophys Res Commun 2000;275:845–849.

    External Resources

  57. Gacad MA, Chen H, Arbelle JE, LeBon T, Adams JS: Functional characterization and purification of an intracellular vitamin D-binding protein in vitamin D-resistant New World primate cells. J Biol Chem 1997;272:8433–8440.
  58. Clements MR, Chalmers TM, Fraser DR: Enterohepatic circulation of vitamin D: A reappraisal of the hypothesis. Lancet 1984;i:1376–1379.
  59. Napoli JL, Mellon WS, Fivizzani MA, Schnoes HK, DeLuca HF: Synthesis and biological activity of 24-hydroxy-25-fluorovitamin D3. A highly potent mediator of calcium metabolism. J Biol Chem 1979;254:2017–2022.
  60. Pavlovitch H, Garabedian M, Balsan S: Calcium-mobilizing effect of large doses of 25-hydroxycholecalciferol in anephric rats. J Clin Invest 1973;52:2656–2659.
  61. Stamp TCB, Round JM, Rowe DJF, Haddad JG: Plasma levels and therapeutic effect of 25-hydroxycholecalciferol in epileptic patients taking anticonvulsant drugs. Br Med J 1972;4:9–12.
  62. Rutherford WE, Blondin J, Hruska K, Kopelman R, Klahr S, Slatopolsky E: Effect of 25-hydroxycholecalciferol on calcium absorption in chronic renal disease. Kidney Int 1975;8:320–324.
  63. Devine A, Wilson SG, Dick IM, Prince RL: Effects of vitamin D metabolites on intestinal calcium absorption and bone turnover in elderly women. Am J Clin Nutr 2002;75:283–288.
  64. Somerman MJ, Neuman WF: Relative effectiveness of vitamin D metabolites in increasing bone mineral solubility. Calcif Tissue Int 1981;33:159–165.
  65. Teitelbaum SL, Bone JM, Stein PM, Gilden JJ, Bates M, Biosseau VC, Avioli LV: Calcifediol in chronic renal insufficiency. JAMA 1976;235:164–167.
  66. Myrtle JF, Norman AW: Vitamin D: A cholecalciferol metabolite highly active in promoting intestinal calcium transport. Science 1971;171:79–82.
  67. Haussler MR, Rasmussen H: The metabolism of vitamin D3 in the chick. J Biol Chem 1972;247:2328–2335.
  68. Soares JH, Kerr JM, Gray RW: 25-Hydroxycholecalciferol in poultry nutrition. Poultry Sci 1995;74:1919–1934.
  69. Yoshimoto Y, Norman AW: Biological activity of vitamin D metabolites and analogs: Dose-response study of 45Ca transport in an isolated chick duodenum perfusion system. J Steroid Biochem 1986;25:905–909.
  70. Holick MF, Baxter LA, Schraufrogel PK, Tavela TE, DeLuca HF: Metabolism and biological activity of 24,25-dihydroxyvitamin D3 in the chick. J Biol Chem 1976;251:397–402.
  71. Blunt JW, Tanaka Y, DeLuca HF: The biological activity of 25-hydroxycholecalciferol, a metabolite of vitamin D3. Proc Natl Acad Sci USA 1968;61:1503–1516.
  72. Suda T, DeLuca HF, Tanaka Y: Biological activity of 25-hydroxyergocalciferol in rats. J Nutr 1970;100:1049–1052.
  73. Miravet L, Redel J, Carre M, Queillé ML, Bordier P: The biological activity of synthetic 25,26-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol in vitamin D-deficient rats. Calcif Tiss Res 1976;21:145–152.
  74. Winter M, Morava E, Simon G, Gyüre A: The effect of vitamin D3 and 25-hydroxycholecalciferol on intestinal transport of calcium in vivo and in vitro. Experientia 1972;28:659–660.
  75. Tanaka Y, Frank H, DeLuca HF: Biological activity of 1,25-dihydroxyvitamin D3 in the rat. Endocrinology 1973;92:417–422.
  76. Mawer EB, Lumb GA, Schaefer K, Stanbury SW: The metabolism of isotopically labelled vitamin D3 in man: The influence of the state of vitamin D nutrition. Clin Sci 1971;40:39–53.
  77. National Research Council: Recommended Dietary Allowances, ed 10. Washington, National Academy Press, 1989.
  78. Chan W, Brown J, Lee SM, Buss DH: Meat, Poultry and Game. Fifth Supplement to the Fifth Edition of McCance and Widdowson’s the Composition of Foods. Cambridge/London, Royal Society of Chemistry/Ministry of Agriculture, Fisheries and Food, 1995.

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