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Vol. 5, No. 4, 2003
Issue release date: July 2003
J Mol Microbiol Biotechnol 2003;5:199–205

The Cyclin in the RNA Polymerase Holoenzyme Is a Target for the Transcriptional Repressor Tup1p in Saccharomyces cerevisiae

Schüller J. · Lehming N.
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore

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The general transcriptional repressor Tup1p requires the cyclin/cyclin-dependent kinase pair Srb11p/Srb10p in the holoenzyme of transcription. We used the split-ubiquitin system to demonstrate that Tup1p interacts with Srb11p in vivo. We confirmed our observation in vitro with the help of purified proteins, and we compared the de-repression effect of deleting TUP1, SRB10, and SRB11 on different promoters. We propose that Tup1p targets the cyclin Srb11p to affect the cyclin-dependent kinase Srb10p.

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