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Vol. 101, No. 3-4, 2003
Issue release date: 2003
Cytogenet Genome Res 101:283–288 (2003)

Steroids, aromatase and sex differentiation of the newt Pleurodeles waltl

Kuntz S. · Chardard D. · Chesnel A. · Grillier-Vuissoz I. · Flament S.
EA 3442 Génétique, Signalisation, Différenciation, Université Henri Poincaré, Nancy (France)

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In the newt Pleurodeles waltl, genetic sex determination obeys female heterogamety (female ZW, male ZZ). In this species as in most of non-mammalian vertebrates, steroid hormones play a key role in sexual differentiation of gonads. In that context, male to female sex reversal can be obtained by treatment of ZZ larvae with estradiol. Male to female sex reversal has also been observed following treatment of ZZ larvae with testosterone, a phenomenon that was called the “paradoxical effect”. Female to male sex reversal occurs when ZW larvae are reared at 32°C during a thermosensitive period (TSP) that takes place from stage 42 to stage 54 of development. Since steroids play an important part in sex differentiation, we focussed our studies on the estrogen-producing enzyme aromatase during normal sex differentiation as well as in experimentally induced sex reversal situations. Our results based on treatment with non-aromatizable androgens, aromatase activity measurements and aromatase expression studies demonstrate that aromatase (i) is differentially active in ZZ and ZW larvae, (ii) is involved in the paradoxical effect and (iii) might be a target of temperature. Thus, the gene encoding aromatase might be one of the master genes in the process leading to the differentiation of the gonad in Pleurodeles waltl.   

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  1. Balthazart J, Baillien M, Ball GF: Phosphorylation processes mediate rapid changes of brain aromatase activity. J Steroid Biochem Mol Biol 79:261–277 (2001).
  2. Bolaffi JL, Lance V, Callard IP, Walsh JM, Idler DR: Identification of 11-ketotestosterone, 11β-hydroxytestosterone and testosterone in plasma of Necturus maculosus (Rafinesque). Gen Comp Endocrinol 38:127–131 (1979).
  3. Borg B: Androgens in teleost fishes. Comp Biochem Physiol 109C:219–245 (1994).
  4. Chardard D, Dournon C: Sex reversal by aromatase inhibitor treatment in the newt Pleurodeles waltl. J exp Zool 283:43–50 (1999).
  5. Chardard D, Desvages G, Pieau C, Dournon C: Aromatase activity in larval gonads of Pleurodeles waltl (urodele amphibia) during normal sex differentiation and during sex reversal by thermal treatment effect. Gen Comp Endocrinol 99:100–107 (1995).
  6. Chardard D, Kuntz S, Chesnel A, Flament S: Effects of androgen on sex differentiation of the urodele Pleurodeles waltl. J exp Zool 296:46–55 (2003).

    External Resources

  7. Dournon C, Houillon C: Démonstration génétique de l’inversion fonctionnelle du phénotype sexuel femelle sous l’action de la température d’élevage chez l’amphibien urodèle Pleurodeles waltlii Michah. Reprod Nutr Dévelop 24:361–378 (1984).
  8. Dournon C, Houillon C: Thermosensibilité de la différenciation sexuelle chez l’amphibien urodèle Pleurodeles waltlii Michah. Conditions pour obtenir l’inversion du phénotype sexuel de toutes les femelles génétiques sous l’action de la température d’élevage. Reprod Nutr Dévelop 25:671–688 (1985).
  9. Dournon C, Houillon C, Pieau C: Temperature sex-reversal in amphibians and reptiles. Int J Dev Biol 34:81–92 (1990).
  10. Ferrier V, Jaylet A, Cayrol C, Gasser F, Buisan JJ: Etude électrophorétique des peptidases érythrocytaires chez Pleurodeles waltlii (Amphibien, Urodèle): mise en évidence d’une liaison avec le sexe. C R Acad Sci Série D 290:571–574 (1980).
  11. Flament S, Kuntz S, Chesnel A, Grillier-Vuissoz I, Tankozic C, Penrad-Mobayed M, Auque G, Shirali P, Schroeder H, Chardard D: Effect of Cadmium on gonadogenesis and metamorphosis in Pleurodeles waltl (urodele amphibian). Aquatic Toxicol 64:143–153 (2003).
  12. Gallien L: Sur la descendance unisexuée d’une femelle de Pleurodeles waltlii Michah. ayant subi pendant sa phase larvaire l’action gynogène du benzoate d’oestradiol. C R Acad Sci Paris Série D 233:828–830 (1951).
  13. Gallien L: Inversion expérimentale du sexe sous l’action des hormones sexuelles chez le triton Pleurodeles waltlii Michah. Analyse des conséquences génétiques. Bull Biol Fr Belg 88:1–51 (1954).
  14. Gallien L: Comparative activity of sexual steroids and genetic constitution in sexual differentiation of amphibian embryos. Gen Comp Endocrinol, Suppl: 346–355 (1962).
  15. Gallien L, Durocher M: Table chronologique du développement chez Pleurodeles waltl. Bull Biol Fr Belg 91:97–114 (1957).
  16. Guiguen Y, Baroiller JF, Ricordel MJ, Iseki K, McMeel OM, Martin SAM, Fostier A: Involvement of estrogens in the process of sex differentiation in two fish species: the rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus). Mol Reprod Develop 54:154–162 (1999).
  17. Hayes TB: Sex determination and primary sex differentiation in amphibians: genetic and developmental mechanisms. J exp Zool 281:373–399 (1998).
  18. Houillon C, Dournon C: Inversion du phénotype sexuel femelle sous l’action d’une température élevée chez l’amphibien urodèle, Pleurodeles waltlii Michah. C R Acad Sci Paris, Série D 286:1475–1478 (1978).
  19. Kuntz S, Chesnel A, Grillier-Vuissoz I, Callier M, Duterque-Coquillaud M, Flament S, Chardard D: Expression of P450 aromatase during sex differentiation and sex reversal in the amphibian Pleurodeles waltl. J Steroid Biochem Mol Biol 84:89–100 (2003).
  20. Lupo di Prisco C, Delrio G, Chieffi G, Bellini Cardinelli L, Polzonetti Magni A: Identification and biosynthesis of steroid hormones in the ovary and fat bodies of female Triturus cristatus carnifex. Comp Biochem Physiol 40 (B):53–60 (1971).
  21. Lupo di Prisco C, Sile CB, Delrio G, Chieffi C: In vitro metabolism of cholesterol-4-14C and testosterone-4-14C in testes and fat bodies of Triturus cristatus carnifex. Comp Biochem Physiol 41(B):245–249 (1972).
  22. Pieau C: Différenciation du sexe en fonction de la température chez les embryons d’Emys orbicularis L. (chélonien); effets des hormones sexuelles. Ann Embryol Morphol 7:365–394 (1974).
  23. Pieau C, Girondot M, Richard-Mercier N, Desvages G, Dorizi M, Zaborski P: Temperature sensitivity of sexual differentiation of gonads in the european pond turtle: hormonal involvement. J exp Zool 270:86–94 (1994).
  24. Pieau C, Dorizzi M, Richard-Mercier N: Temperature dependent sex determination and gonadal differentiation in reptiles. In: Genes and mechanisms in vertebrate sex determination. EXS 117–141 (2001).
  25. Scheib D: Effects and roles of estrogens in avian gonadal differentiation. Differentiation 23 (suppl):587–592 (1983).
  26. Wallace H, Badawy GMI, Wallace BMN: Amphibian sex determination and sex reversal. Cell Mol Life Sci 55:901–909 (1999).
  27. Wibbels T, Crews D: Specificity of steroid hormone-induced sex determination in a turtle. J Endocrinol 133:121–129 (1992).
  28. Zaborski P: Temperature and estrogen dependent changes of sex phenotype and HY antigen expression in gonads of a newt. Prog Clin Biol Res 217A:163–169 (1986).

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