Expression of Visinin-Like Protein-3 in Mouse KidneyPribanic S. · Loffing J. · Madjdpour C. · Bacic D. · Gisler S. · Braunewell K.-H. · Biber J. · Murer H.
Institutes of aPhysiology and bAnatomy, University of Zürich, Zürich, Switzerland, and cInstitute for Physiology, Humboldt University Berlin, Berlin, Germany
In renal proximal brush borders the Na/Pi cotransporter NaPi-IIa is part of a heteromultimeric complex including the PDZ proteins PDZK1 and NHERF1, which interact with the C terminus of NaPi-IIa. In this study, a yeast two-hybrid screen against the N terminus of the Na/Pi cotransporter NaPi-IIa was performed. Thereby we identified visinin-like protein-3 (VILIP-3), a member of neuronal calcium sensors. In this study, expression and protein localization of VILIP-3 in the mouse kidney was performed by immunofluorescence and RT-PCR using laser-assisted microdissected nephron segments. VILIP-3 was found to be abundant in distal and collecting ducts where it partly colocalized with calbindin D28K. In addition VILIP-3 was observed in the brush borders of proximal tubular S1 and S3 segments of both superficial and deep nephrons.
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