Journal Mobile Options
Table of Contents
Vol. 85, No. 1, 2004
Issue release date: March 2004
Section title: Original Paper
Biol Neonate 2004;85:61–67
(DOI:10.1159/000074960)

Transient Decrease in the CD45RA Expression on T Lymphocytes in Neonates with Fetal Distress

Mizobe N. · Sugita K. · Tezuka T. · Sakamoto M. · Fukada Y. · Hoshi K. · Nakazawa S.
Departments of aPediatrics, bClinical and Laboratory Medicine, and cObstetrics and Gynecology, Faculty of Medicine, University of Yamanashi, Yamanashi, and dDivision of Neonatology, Perinatal Center, YamanashiPrefectural Central Hospital, Yamanashi, Japan

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restriction apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/24/2003
Published online: 3/12/2004

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO

Abstract

To determine the expression of CD45 isoforms on T lymphocytes in neonates with fetal distress and to evaluate its diagnostic accuracy, peripheral blood samples were examined in 53 neonates who were classified into one of three groups: group I: ‘control’ group (n = 23), group II: ‘mild distress’ group (n = 15), and group III: ‘moderate distress’ group (n = 15). The expression of CD3 (mean ± SD 24.2 ± 10.1%), CD4 (23.0 ± 5.7%), and CD45RA (27.3 ± 9.6%) on total lymphocytes and the expression of CD45RA on CD4+ T lymphocytes (13.7 ± 4.7%) in group III were significantly lower than in the other two groups 0–3 days after birth. Sensitivity and specificity of the CD45RA expression on CD4+ T lymphocytes for discrimination of group III were calculated as 0.79 and 1.0, respectively, when the cutoff value was 22.7%. The low CD3, CD4, and CD45RA expression returned to normal levels 10 days and more after birth. There were no differences in the CD8 and CD45RO expression between the groups. We conclude that CD4+ T lymphocytes from neonates with fetal distress show a transient decrease in the CD45RA expression without an increase in the CD45RO expression, and, therefore, analysis of the CD45 isoform expression is useful for laboratory evaluation of fetal distress.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/24/2003
Published online: 3/12/2004

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Thomas ML: The leukocyte common antigen family. Annu Rev Immunol 1989;7:339–369.
  2. Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL-1. Immunology 1986;58:63–70.
  3. Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol 1988;140:2171–2178.
  4. Rudd CE, Morimoto C, Wong LL, Schlossman SF: The subdivision of the T4 (CD4) subset on the basis of the differential expression of L-C/T200 antigens. J Exp Med 1987;166:1758–1773.
  5. Streuli M, Hall LR, Saga Y, Schlossman SF, Saito H: Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J Exp Med 1987;166:1548–1566.
  6. De Paoli P, Battistin S, Santini GF: Age-related changes in human lymphocyte subsets: Progressive reduction of the CD4 CD45R (suppressor inducer) population. Clin Immunol Immunopathol 1988;48:290–296.
  7. Miyawaki T, Kasahara Y, Kanegane H, Ohta K, Yokoi T, Yachie A, Taniguchi N: Expression of CD45RO (UCHL1) by CD4+ and CD8+ T cells as a sign of in vivo activation in infectious mononucleosis. Clin Exp Immunol 1991;83:447–451.
  8. Pinto L, Covas MJ, Victorino RM: Loss of CD45RA and gain of CD45RO after in vitro activation of lymphocytes from HIV-infected patients. Immunology 1991;73:147–150.
  9. Mimura T, Fernsten P, Jarjour W, Winfield JB: Autoantibodies specific for different isoforms of CD45 in systemic lupus erythematosus. J Exp Med 1990;172:653–656.
  10. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF: The isolation and characterization of the human suppressor inducer T cell subset. J Immunol 1985;134:1508–1515.
  11. Rothstein DM, Saito H, Streuli M, Schlossman SF, Morimoto C: The alternative splicing of CD45 tyrosine phosphatase is controlled by negative regulatory trans-acting splicing factors. J Biol Chem 1992;267:7139–7147.
  12. Sugita K, Hirose T, Rothstein DM, Donahue C, Schlossman SF, Morimoto C: CD27, a member of the nerve growth factor receptor family, is preferentially expressed on CD45RA+ CD4 T cell clones and involved in distinct immunoregulatory functions. J Immunol 1992;149:3208–3216.
  13. Michie C, Harvey D: Can expression of CD45RO, a T-cell surface molecule, be used to detect congenital infection? Lancet 1994;343:1259–1260.
  14. Tezuka T, Sugita K, Mizobe N, Goi K, Miyamoto N, Nakamura M, Kagami K, Yokoyama T, Nakazawa S: Transient increase in CD45RO expression on T lymphocytes in infected newborns. Pediatr Res 1998;43:283–290.
  15. Robertson C, Finer N: Term infants with hypoxic-ischemic encephalopathy: Outcome at 3.5 years. Dev Med Child Neurol 1985;27:473–484.
  16. Freeman JM, Nelson KB: Intrapartum asphyxia and cerebral palsy. Pediatrics 1988;82:240–249.
  17. Sykes GS, Molloy PM, Johnson P, Gu W, Ashworth F, Stirrat GM, Turnbull AC: Do Apgar scores indicate asphyxia? Lancet 1982;1:494–496.
  18. Parer JT, Livingston EG: What is fetal distress? Am J Obstet Gynecol 1990;162:1421–1427.
  19. Low JA: Intrapartum fetal asphyxia: Definition, diagnosis, and classification. Am J Obstet Gynecol 1997;176:957–959.
  20. American Academy of Pediatrics, American College of Obstetricians and Gynecologists: Relationship between perinatal factors and neurologic outcome; in Poland RL, Freeman RK (eds): Guidelines for Perinatal Care, ed 3. Elk Grove Village, American Academy of Pediatrics, 1992, pp 221–224.
  21. Erkeller-Yuksel FM, Deneys V, Yuksel B, Hannet I, Hulstaert F, Hamilton C, Mackinnon H, Stokes LT, Munhyeshuli V, Vanlangendonck F, De Bruyere M, Bach BA, Lydyard PM: Age-related changes in human blood lymphocyte subpopulations. J Pediatr 1992;120:216–222.
  22. Raes M, Alliet P, Gillis P, Zimmermann A, Kortleven J, Magerman K, Peeters V, Rummens JL: Lymphocyte subpopulations in healthy newborn infants: Comparison of cord blood values with values five days after birth. J Pediatr 1993;123:465–467.
  23. Baker DA, Hameed C, Tejani N, Thomas J, Dattwyler R: Lymphocyte subsets in the neonates of preeclamptic mothers. Am J Reprod Immunol Microbiol 1987;14:107–109.
  24. Berry SM, Fine N, Bichalski JA, Cotton DB, Dombrowski MP, Kaplan J: Circulating lymphocyte subsets in second- and third-trimester fetuses: Comparison with newborns and adults. Am J Obstet Gynecol 1992;167:895–900.
  25. Thilaganathan B, Meher-Homji N, Nicolaides KH: Labor: An immunologically beneficial process for the neonate. Am J Obstet Gynecol 1994;171:1271–1272.
  26. Boyer C, Auphan N, Luton F, Malburet JM, Barad M, Bizozzero JP, Reggio H, Schmitt-Verhulst AM: T cell receptor/CD3 complex internalization following activation of a cytolytic T cell clone: Evidence for a protein kinase C-independent staurosporine-sensitive step. Eur J Immunol 1991;21:1623–1634.
  27. Green DW, Hendon B, Mimouni FB: Nucleated erythrocytes and intraventricular hemorrhage in preterm neonates. Pediatrics 1995;96:475–478.