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Vol. 60, No. 1, 1999
Issue release date: January–February 1999

Augmentation of Water-Immersion Stress- Induced Gastric Mucosal Lesions in BALB/c Mice Infected with Helicobacter felis

Matsushima Y. · Kinoshita Y. · Watanabe M. · Hassan S. · Fukui H. · Maekawa T. · Okada A. · Kawanami C. · Kishi K. · Watanabe N. · Nakao M. · Chiba T.
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Abstract

Background and Aims: Inoculation of Helicobacter felis into the murine stomach has been reported to induce chronic gastric inflammation and may be a model of Helicobacter pylori-induced chronic gastritis. In this study, to characterize H. felis-induced gastritis, the gastric production of interleukin-1β (IL-1β) and hepatocyte growth factor (HGF) was measured in mice. Methods: Gastric mucosal lesions were induced in H. felis-infected BALB/c mice by water-immersion stress. The severity score of gastric erosions per stomach was measured as the sum of the length of erosions. Gene expression of IL-1β and HGF were analyzed by Northern blot analysis and production of HGF was examined using the enzyme immunoassay method. Results: Water-immersion stress induced gastric mucosal lesions accompanied by increased expression of IL-1β mRNA. H. felis infection evoked enhanced expression of IL-1β and HGF genes. When H. felis-infected mice were stressed by water immersion, the mucosal lesions were more severe than those in non-infected mice. Moreover, IL-1β gene expression as well as HGF production was further increased. Conclusions: Although H. felis inoculation did not cause gastric mucosal erosions by itself, it augmented the stress-induced erosions.



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