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Vol. 48, No. 2, 2004
Issue release date: March–April 2004
Ann Nutr Metab 2004;48:109–117

Impact of Feeding High-Iron Rice on Plasma Iron, Hemoglobin and Red Blood Cell Variables of Early-Weaned Piglets

A Pilot Study

Schaffer S. · Pallauf J. · Krawinkel M.B.
aInstitute of Nutritional Sciences and bInstitute of Animal Nutrition and Nutrition Physiology, Justus Liebig University, Giessen, Germany

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Severe iron deficiency is a major nutritional problem encountered throughout the world. We assessed the effect of a conventionally bred, high-iron rice variety on plasma iron, hemoglobin, and red blood cell variables of early-weaned piglets during a 33-day feeding trial. 26-day-old male piglets were assigned to 3 treatment groups: group 1 = low-iron rice + low-iron supplementary feed; group 2 = high-iron rice + low-iron supplementary feed, and group 3 = low-iron rice + high-iron supplementary feed. Plasma iron, hemoglobin and red blood cell variables were measured on days 8, 16, 23, 30, and 33. Feed intake and weight gain were not significantly different between study groups. No significant differences in the iron-related parameters analyzed were found between the piglets of groups 1 and 2, except in red blood cells. Modifications regarding study design, study duration and subject’s growth rate are recommended to increase the possibility of detecting changes in the iron status triggered by diets having small differences in dietary iron.

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  1. World Health Organization: The World Health Report 1998. Life in the 21st Century: A Vision for All. Geneva, WHO, 1998.
  2. Mason JB, Lotfi M, Dalmiya N, Sethuraman K, Deitchler M: The Micronutrient Report – Current Progress and Trends in the Control of Vitamin A, Iodine, and Iron Deficiencies. Ottawa, Micronutrient Initiative, 2001.
  3. Brown KH, Wuehler SE (eds): Zinc and Human Health – Results of Recent Trials and Implications for Program Interventions and Research. Ottawa, Micronutrient Initiative, 2001.
  4. Lynch S, Green R: Assessment of nutritional anemias; in Ramakrishnan U (ed): Nutritional Anemias. Boca Raton, CRC Press, 2001, pp 23–42.
  5. Beard JL: Iron biology and immune function, muscle metabolism and neuronal functioning. J Nutr 2001;131:568S–580S.
  6. Shankar AH, Prasad AS: Zinc and immune function: The biological basis of altered resistance to infection. Am J Clin Nutr 1998;68(suppl):447S–463S.
  7. Gilbert C, Foster A: Childhood blindness in the context of the Vision 2020 – The right to sight. Bull World Health Organ 2001;79:227–232.
  8. Nilson A, Piza J: Food fortification – A tool for fighting hidden hunger. Food Nutr Bull 1998;19:49–600.
  9. Ahmed F, Khan MR, Jackson AA: Concomitant supplemental vitamin A enhances the response to weekly supplemental iron and folic acid in anemic teenagers in urban Bangladesh. Am J Clin Nutr 2001;74:108–115.
  10. Gibson RS, Hotz C: Dietary diversification/modification strategies to enhance micronutrient content and bioavailability of diets in developing countries. Br J Nutr 2001;85(suppl 2):S159–S166.
  11. Nurdia DS, Sumarni S, Suyoko, Hakim M, Winkvist A: Impact of intestinal helminth infection on anemia and iron status during pregnancy: A community based study in Indonesia. Southeast Asian J Trop Med Public Health 2001;32:14–22.
  12. Solon FS, Klemm RDW, Sanchez L, Darnton-Hill I, Craft NE, Christian P, West KP: Efficacy of a vitamin A-fortified wheat-flour bun on the vitamin A status of Filipino schoolchildren. Am J Clin Nutr 2000;72:738–744.
  13. Darnton-Hill I: Overview: Rationale and elements of a successful food-fortification programme. Food Nutr Bull 1998;19:92–100.
  14. United Nations: World Declaration and Plan of Action. New York, World Food Summit, Sept 1990.
  15. Bouis H: Enrichment of food staples through plant breeding: A new strategy for fighting micronutrient malnutrition. Nutr Rev 1996;54:131–137.
  16. Graham RD, Senadhira D, Ortiz-Monasterio I: A strategy for breeding staple-food crops with high micronutrient density. Soil Sci Plant Nutr 1997;43:1153–1157.
  17. Datta SK: Transgenic cereals: Oryza sativa (rice); in Vasil IK (ed): Molecular Improvement of Cereal Crops. Amsterdam, Kluwer Academic, 1998, pp 149–187.
  18. Frossad E, Bucher M, Mächler F, Mozafar A, Hurrell R: Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. J Sci Food Agric 2000;80:861–879.
  19. Fritschel H: Fighting hidden hunger; in Pinstrup-Andersen P, Pandya-Lorch R (eds): The Unfinished Agenda – Perspectives on Overcoming Hunger, Poverty, and Environmental Degradation. Washington, International Food Policy Research Center, 2001 pp 31–36.
  20. Bouis HE, Graham RD, Welch RM: The Consultative Group on International Agricultural Research (CGIAR) Micronutrients Project: Justification and objectives. Food Nutr Bull 2000;21:374–381.
  21. Khush GS: Origin, dispersal, cultivation and variation of rice. Plant Mol Biol 1997;35:25–34.
  22. Gregorio GB, Senadhira D, Htut H, Graham RD: Breeding for trace mineral density in rice. Food Nutr Bull 2000;21:382–386.
  23. Welch RM, House WA, Beebe S, Senadhira D, Gregorio GB, Cheng Z: Testing iron and zinc bioavailability in genetically enriched beans (Phaseolus vulgaris L.) and rice (Oryza sativa L.) in a rat model. Food Nutr Bull 2000;21:428–433.
  24. Miller ER, Ullrey DE: The pig as a model for human nutrition. Annu Rev Nutr 1987;7:361–382.
  25. Moughan PJ, Birtles MJ, Cranwell PD, Smith WC, Pedraza M: The piglet as a model animal for studying aspects of digestion and absorption in milk-fed human infants. World Rev Nutr Diet 1992;67:40–113.
  26. Egeli AK, Framstad T, Morberg H: Clinical biochemistry, haematology and body weight in piglets. Acta Vet Scand 1998;39:381–393.
  27. Kraft W, Dürr UM, Klee W, Bostedt H, Heinritzi K: Hämatologie; in Kraft W, Dürr U (eds): Klinische Labordiagnostik in der Tiermedizin. Stuttgart, Schattauer, 1995, pp 38–67.
  28. Dvorák M: Diagnostik des Eisenmangels beim Saugferkeln. II. Das Serumeisen und die Eisenbindungskapazität des Blutserums. Wien Tierärztl Monatsschr 1964;51:596–606.
  29. Kirchgessner M: Tierernährung, ed 10. Frankfurt/Main, DLG, 1997.
  30. Tobin BW, Beard JL, Kenney WL: Exercise training alters feed efficiency and body composition in iron deficient rats. Med Sci Sports Exerc 1993;25:52–59.
  31. Aukett MA, Parks YA, Scott PH, Wharton BA: Treatment with iron increases weight gain and psychomotor development. Arch Dis Child 1986;61:849–857.
  32. Angeles IT, Schultink WJ, Matulessi P, Gross R, Sastroamidjojo S: Deceasing rate of stunting among anemic Indonesian preschool children through iron supplementation. Am J Clin Nutr 1993;53:339–342.
  33. Steinhardt M, Bünger U, Furcht G, Grätsch U, Pape G: Eisenmangel bei Ferkeln – Ein altes Problem aus heutiger Sicht. Tierzucht 1985;39:466–470.
  34. Allen LH: Nutritional influences on linear growth: A general review. Eur J Clin Nutr 1994;48(suppl 1):S75–S89.
  35. Lemacher S, Bostedt H: Zur Entwicklung der Plasma-Fe-Konzentration und des Hämoglobingehaltes beim Ferkel in den ersten drei Lebenstagen und zur Bedeutung der pränatalen Anämie. Tierärztl Prax 1994;22:39–45.
  36. Egeli AK, Framstad T: Evaluation of the efficacy of perorally administered glutamic acid-chelated iron and iron-dextran injected subcutaneously in Duroc and Norwegian Landrace piglets. Zentralbl Veterinärmed A 1998;45:53–61.
  37. Beard JL, Dawson H, Piñero DJ: Iron metabolism. A comprehensive review. Nutr Rev 1996;54:295–317.
  38. Bothwell TH: Overview and mechanisms of iron regulation. Nutr Rev 1995;53:237–245.
  39. Roy CN, Enns CA: Iron homeostasis: New tales from the crypt. Blood 2000;96:4020–4027.
  40. Kooistra MP, van Es A, Struyvenberg A, Marx JJ: Iron metabolism in patients with the anaemia of end-stage renal disease during treatment with recombinant human erythropoietin. Br J Haematol 1991;79:634–639.
  41. Wood RJ, Han O: Recently identified molecular aspects of intestinal iron absorption. J Nutr 1998;128:1841–1844.
  42. Yeh KY, Yeh M, Watkins JA, Rodriguez-Paris J, Glass J: Dietary iron induces rapid changes in rat intestinal divalent metal transporter expression. Am J Physiol Gastrointest Liver Physiol 2000;279:G1070–G1079.
  43. Murray-Kolb LE, Takaiwa F, Goto F, Yoshihara T, Theil EC, Beard JL: Transgenic rice is a source of iron for iron-depleted rats. J Nutr 2002;132:957–960.
  44. Forth W, Rummel W: Iron absorption. Physiol Rev 1973;53:724–792.
  45. Dallmann PR: Biochemical basis for the manifestation of iron deficiency. Annu Rev Nutr 1986;6:13–40.
  46. Lopez HW, Vallery F, Levrat-Verny MA, Coudray C, Demigne C, Remesy C: Dietary phytic acid and wheat bran enhance mucosal phytase activity in rat small intestine. J Nutr 2000;130:2020–2025.
  47. Rao NSB, Prasad JS, Sarathy CV: An animal model to study iron bioavailability from human diets. Br J Nutr 1977;37:451–456.
  48. Wienk KJH, Marx JJM, Beynen AC: The concept of iron bioavailability and its assessment. Eur J Nutr 1999;38:51–75.
  49. Forbes AL, Arnaud MJ, Chichester CO, Cook JD, Harrison BN, Hurrell RF, Kahn SG, Morris ER, Tanner JT, Whittaker P, et al: Comparison of in vitro, animal, and clinical determinations of iron bioavailability: International Nutritional Anemia Consultative Group Task Force report on iron bioavailability. Am J Clin Nutr 1989;49:225–238.
  50. Minihane AM, Rimbach G: Iron absorption and iron binding and anti-oxidant properties of phytic acid. Int J Food Sci Technol 2002;37:741–748.
  51. Lopez HW, Leenhardt F, Coudray C, Remesy C: Minerals and phytic acid interactions: Is it a real problem for human nutrition ? Int J Food Sci Technol 2002;37:727–739.
  52. Glahn RP, Cheng Z, Welch RM: Comparison of iron bioavailability from 15 rice genotypes: Studies using an in vitro digestion/Caco-2 cell culture model. J Agric Food Chem 2002;50:3586–3591.
  53. Shamsuddin AM: Anti-cancer function of phytic acid. Int J Food Sci Technol 2002;37:769–782.
  54. Agricultural Research Council: The Nutrient Requirements of Pigs. Slough, Commonwealth Agricultural Bureaux, 1981.
  55. Plonait H, Bickhardt K (eds): Lehrbuch der Schweinekrankheiten. Berlin, Parey, 1997.
  56. Frolich W, Lyso A: Bioavailability of iron from wheat bran in pigs. Am J Clin Nutr 1983;37:31–36.
  57. Andews NC: Disorders of iron metabolism. N Engl J Med 1999;341:1986–1995.
  58. Cook JD, Finch CA: Assessing iron status of a population. Am J Clin Nutr 1979;32:2115–2119.
  59. Hershko C, Bar-Or D, Gaziel Y, Naparstek E, Konijn AM, Grossowicz N, Kaufmann N, Izak G: Diagnosis of iron deficiency anemia in a rural population of children. Relative usefulness of serum ferritin, red cell protoporphyrin, red cell indices, and transferrin saturation determinations. Am J Clin Nutr 1981;34:1600–1610.
  60. Gibson RS: Principles of Nutritional Assessment. New York, Oxford University Press, 1990, pp 349–376.
  61. Worwood M: The laboratory assessment of iron status – An update. Clin Chim Acta 1997;259:3–23.
  62. Lipschitz DA, Cook JD, Finch CA: Serum ferritin as a measure of iron store. N Engl J Med 1974;290:1213–1216.
  63. Cook JD: Clinical evaluation of iron deficiency. Semin Hematol 1982;19:6–18.
  64. Hallberg L: Bioavailability of dietary iron in man. Annu Rev Nutr 1981;1:123–147.
  65. Herbert V, Jayatilleke E, Shaw S, Rosman AS, Giardina P, Grady RW, Bowman B, Gunter EW: Serum ferritin iron, a new test, measures human body iron stores unconfounded by inflammation. Stem Cells 1997;15:291–296.
  66. Waters HM, Seal LH: A systematic approach to the assessment of erythropoiesis. Clin Lab Haem 2001;23:271–283.

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