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Vol. 105, No. 2-4, 2004
Issue release date: 2004
Section title: Genome Organisation and Function
Cytogenet Genome Res 105:292–301 (2004)
(DOI:10.1159/000078201)

Spatial genome organization during T-cell differentiation

Kim S.H. · McQueen P.G. · Lichtman M.K. · Shevach E.M. · Parada L.A. · Misteli T.
aNational Cancer Institute, NIH; bMathematical and Statistical Laboratory, DCB, CIT, NIH, and cNational Institute of Allergy and Infectious Diseases, Laboratory of Immunology, Bethesda, MD (USA)

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Article / Publication Details

First-Page Preview
Abstract of Genome Organisation and Function

Published online: 7/14/2004

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

The spatial organization of genomes within the mammalian cell nucleus is non-random. The functional relevance of spatial genome organization might be in influencing gene expression programs as cells undergo changes during development and differentiation. To gain insight into the plasticity of genomes in space and time and to correlate the activity of specific genes with their nuclear position, we systematically analyzed the spatial genome organization in differentiating mouse T-cells. We find significant global reorganization of centromeres, chromosomes and gene loci during the differentiation process. Centromeres were repositioned from a preferentially internal distribution in undifferentiated cells to a preferentially peripheral position in differentiated CD4+ and CD8+ cells. Chromosome 6, containing the differentially expressed T-cell markers CD4 and CD8, underwent differential changes in position depending on whether cells differentiated into CD4+ or CD8+ thymocytes. Similarly, the two marker loci CD4 and CD8 showed distinct behavior in their position relative to the chromosome 6 centromere at various stages of differentiation. Our results demonstrate that significant spatial genome reorganization occurs during differentiation and indicate that the relationship between dynamic genome topology and single gene regulation is highly complex.   


Article / Publication Details

First-Page Preview
Abstract of Genome Organisation and Function

Published online: 7/14/2004

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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