Table of Contents
To view the fulltext, log-in or choose pay-per-view options:
Get Access

Spatial genome organization during T-cell differentiation

Kim S.H.a · McQueen P.G.b · Lichtman M.K.c · Shevach E.M.c · Parada L.A.a · Misteli T.a
aNational Cancer Institute, NIH; bMathematical and Statistical Laboratory, DCB, CIT, NIH, and cNational Institute of Allergy and Infectious Diseases, Laboratory of Immunology, Bethesda, MD (USA) Cytogenet Genome Res 105:292–301 (2004) (DOI:10.1159/000078201)

Abstract

The spatial organization of genomes within the mammalian cell nucleus is non-random. The functional relevance of spatial genome organization might be in influencing gene expression programs as cells undergo changes during development and differentiation. To gain insight into the plasticity of genomes in space and time and to correlate the activity of specific genes with their nuclear position, we systematically analyzed the spatial genome organization in differentiating mouse T-cells. We find significant global reorganization of centromeres, chromosomes and gene loci during the differentiation process. Centromeres were repositioned from a preferentially internal distribution in undifferentiated cells to a preferentially peripheral position in differentiated CD4+ and CD8+ cells. Chromosome 6, containing the differentially expressed T-cell markers CD4 and CD8, underwent differential changes in position depending on whether cells differentiated into CD4+ or CD8+ thymocytes. Similarly, the two marker loci CD4 and CD8 showed distinct behavior in their position relative to the chromosome 6 centromere at various stages of differentiation. Our results demonstrate that significant spatial genome reorganization occurs during differentiation and indicate that the relationship between dynamic genome topology and single gene regulation is highly complex.   

 

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50