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Vol. 134, No. 2, 2004
Issue release date: June 2004

Qualitative and Quantitative Evaluation of Bird-Specific IgG Antibodies

Lopata A.L. · Schinkel M. · Potter P.C. · Jeebhay M.F. · Hashemi C. · Johansson S.G.O. · van Hage-Hamsten M.
Int Arch Allergy Immunol 2004;134:173–178 (DOI:10.1159/000078651)
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Abstract

Background: Exposure to organic dust produced by birds often gives rise to an immune response, e.g. IgG antibodies, but intense exposure can lead to high concentrations of IgG antibodies and the development of allergic alveolitis, often known as ‘bird fancier’s lung’. The aim of this study was to establish the distribution of bird-specific IgG antibodies in exposed and nonexposed individuals and compare a nonquantitative and quantitative method in evaluating raised levels of IgG antibodies. Methods: Sera were collected in Sweden and South Africa and levels of IgG antibodies specific to pigeon, budgerigar and parrot antigens were quantified using the UniCAP system. Results were compared to the precipitation in gel assay. The IgG antibody values of symptomatic patients without precipitating antibodies (non-PP group; n = 51) and patients with precipitating antibodies (PP group; n = 34) were analyzed and compared to nonexposed asymptomatic blood donors (BD group; n = 73) and environmentally exposed pigeon breeders (n = 11). Results: The IgG antibody response of the analyzed groups in Sweden and South Africa did not vary significantly from each other. IgG antibody responses were the strongest to pigeon antigens with clear increased IgG antibody levels in the PP group [geometric mean (GM) 603 mg/l] compared to the non-PP (GM 6.9 mg/l) and BD group (GM 5.0 mg/l). Threshold values, calculated as the GM value from the BD group plus 3 standard deviations (99% confidence interval), were 9.8, 10.8 and 10.0 mg/l for pigeons, budgerigars and parrots, respectively. Comparison of the two methods resulted in a good concordance with a level of agreement of 94.1% (kappa statistic = 0.83). Conclusions: The UniCAP system for the detection of bird-specific IgG antibodies is a highly reproducible, generally available, quantitative method for routine diagnostic testing and monitoring of exposed subjects with a very high level of agreement to the precipitating gel assay.



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References

  1. McSharry C, Anderson K, Bourke SJ, Boyd G: Takes your breath away – The immunology of allergic alveolitis. Clin Exp Immunol 2002;128:3–9.
  2. Johansson SG, Hourihane JO, Bousquet J, et al: A revised nomenclature for allergy. An EAACI position statement from the EAACI nomenclature task force. Allergy 2001;56:813–824.
  3. Bourke S, Boyd G: Pigeon fancier’s lung. BMJ 1997;315:70–71.
  4. Tasaka S, Kanazawa M, Kawai C, et al: Fatal diffuse alveolar damage from bird fanciers’ lung. Respiration 1997;64:307–309.
  5. Curtis L, Lee BS, Cai D, et al: Pigeon allergens in indoor environments: A preliminary study. Allergy 2002;57:627–631.
  6. Yoshizawa Y, Miyake S, Sumi Y, Hisauchi K, Sato T, Kurup VP: A follow-up study of pulmonary function tests, bronchoalveolar lavage cells, and humoral and cellular immunity in bird fancier’s lung. J Allergy Clin Immunol 1995;96:122–129.
  7. McSharry C: B lymphocytes in allergic alveolitis. Clin Exp Allergy 2003;33:159–162.
  8. Rodriguez de Castro F, Carrillo T, Castillo R, Blanco C, Diaz F, Cuevas M: Relationships between characteristics of exposure to pigeon antigens. Clinical manifestations and humoral immune response. Chest 1993;103:1059–1063.
  9. Obexer G, Ebner H, Feldner H, Rumpold H, Kraft D: Extrinsic allergic alveolitis: Comparison of 2 methods (Ouchterlony gel precipitation and ELISA) for antibody detection in routine diagnosis. Wien Klin Wochenschr 1983;95:789–791.
  10. McSharry C, Anderson K, Boyd G: A review of antigen diversity causing lung disease among pigeon breeders. Clin Exp Allergy 2000;30:1221–1229.
  11. Aberer W, Woltsche M, Woltsche-Kahr I, Kranke B: IgG antibodies typical for extrinsic allergic alveolitis – An inter-laboratory quality assessment. Eur J Med Res 2001;6:498–504.
  12. Paganelli R, Ansotegui IJ, Sastre J, et al: Specific IgE antibodies in the diagnosis of atopic disease. Clinical evaluation of a new in vitro test system, UniCAP, in six European allergy clinics. Allergy 1998;53:763–768.
  13. Cots P, Pena JM, Botey J, Eseverri JL, Marin A, Ras R: Determination of total and specific IgE using UNICAP 100: Comparative study with the CAP system. Allergol Immunopathol (Madr) 1998;26:223–227.
  14. Simpson C, Shirodaria PV, Evans JP, Simpson DI, Stanford CF: Comparison of immunodiffusion and enzyme linked immunosorbent assay in the detection of abnormal antibodies in pigeon breeder’s disease. J Clin Pathol 1992;45:490–493.
  15. Tauer-Reich I, Fruhmann G, Czuppon AB, Baur X: Allergens causing bird fancier’s asthma. Allergy 1994;49:448–453.
  16. Martinez-Cordero E, Aguilar Leon DE, Retana VN: IgM antiavian antibodies in sera from patients with pigeon breeder’s disease. J Clin Lab Anal 2000;14:201–207.
  17. Yoshizawa Y, Miyashita Y, Inoue T, et al: Sequential evaluation of clinical and immunological findings in hypersensitivity pneumonitis: Serial subclass distribution of antibodies. Clin Immunol Immunopathol 1994;73:330–337.
  18. Todd A, Coan R, Allen A: Pigeon breeders’ lung; IgG subclasses to pigeon intestinal mucin and IgA antigens. Clin Exp Immunol 1993;92:494–499.
  19. Baldwin CI, Stevens B, Connors S, et al: Pigeon fanciers’ lung: The mucin antigen is present in pigeon droppings and pigeon bloom. Int Arch Allergy Immunol 1998;117:187–193.
  20. Andersen P, Schonheyder H: Antibodies to hen and duck antigens in poultry workers. Clin Allergy 1984;14:421–428.
  21. Banales JL, Vazquez L, Mendoza F, et al: On the correct determination of reference values for serum antibodies against pigeon serum antigen using a group of healthy blood donors. Arch Med Res 1997;28:289–291.


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