Vol. 71, No. 4, 2004
Issue release date: July–August 2004
Respiration 2004;71:360–366
Clinical Investigations
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Isolation of Fungi, Especially Exophiala dermatitidis, in Patients Suffering from Cystic Fibrosis

A Prospective Study

Horré R.a,b · Schaal K.P.a · Siekmeier R.a,b · Sterzik B.b · de Hoog G.S.d · Schnitzler N.b,c
aInstitute for Medical Microbiology and Immunology, University of Bonn, bFederal Institute for Drugs and Medical Devices, Bonn, and cInstitute for Medical Microbiology, RWTH, Aachen, Germany; dCentraalbureau voor Schimmelcultures Utrecht, The Netherlands
email Corresponding Author


 goto top of outline Key Words

  • Cystic fibrosis
  • Black yeasts
  • Exophiala dermatitidis
  • Sputum

 goto top of outline Abstract

Background: Patients with cystic fibrosis (CF) are at an increased risk of pulmonary colonisation by opportunistic micro-organisms. Using specialised methods, the black yeast Exophiala dermatitidis could consistently be cultured from CF patients. Isolation rates from sputum samples ranged between 1.8 and 15.7%. Occasionally, infection could be recognised. Objectives: This study aimed at investigating the isolation rates of E. dermatitidis in samples taken from CF patients at the University of Bonn, Germany. Methods: Altogether, 439 respiratory specimens taken from 81 CF patients were screened for the occurrence of E. dermatitidis over a period of 18 months. For the selective isolation of this fungus erythritol-chloramphenicol agar (ECA) produced in house was applied. Results: The isolation rate of E. dermatitidis was 1.1% from all specimens, 1.6% from all sputum samples and 6.2% in all patients examined. Conclusions: Prior to the introduction of ECA, E. dermatitidis had never been isolated in our laboratory, either from CF, or from any other patient. During this study, E. dermatitidis was found to colonise the respiratory tract of some CF patients. The use of additional selective culture media is necessary for the recognition of uncommon fungi, e.g. E. dermatitidis, in CF patients.

Copyright © 2004 S. Karger AG, Basel

goto top of outline Introduction

The viscous sputum of patients suffering from cystic fibrosis (CF) is frequently contaminated by certain facultative pathogens (e.g. Pseudomonas aeruginosa, Aspergillus fumigatus and Candida albicans) due to colonisation of the respiratory tract. In 1990 and 1992, pulmonary infections due to the black yeast Exophiala dermatitidis were described for the first time [1, 2]. A new culture medium was developed for the efficient recovery of this species, the erythritol-chloramphenicol agar (ECA) [3]. Since then, the fungus was found frequently in sputum samples of CF patients. This paper reports on the isolation rate of E. dermatitidis in CF patients in Bonn, Germany, during a period of 18 months.


goto top of outline Materials and Methods

During a period of 18 months, 439 specimens (315 sputum samples, 122 oral swabs, 1 nasal swab, 1 tracheal secretion) from patients suffering from CF were cultured for the presence of E. dermatitidis. The samples were taken from 81 CF patients, among whom there were 6 sister-brother pairs. The male-to-female ratio was 1.1/1 (42 male/39 female patients). Patients’ ages ranged between 3 months and 42 years (mean age: 18 years). A minimum of 1 specimen and a maximum of 12 specimens were analysed from each patient. Specimens from an individual patient were collected at intervals ranging from a few weeks up to a few months.

The specimens were delivered to the Institute by a courier from the Children’s Hospital of the University of Bonn. They were plated within 2–4 h on to the culture media shown in table 1.


Table 1. Culture media used in this study

For the selective isolation of E. dermatitidis, ECA produced in house [3] was applied. Strain CBS 148.90 (CBS Culture Collection) was used for quality assessment of every batch of ECA. The medium was stored at 8°C until use, for a maximum of 14 days. After inoculation, the plates were incubated at 36 ± 1°C for 28–30 days and examined for fungal growth every 2–3 days. Black fungi were identified down to species level using morphological criteria, growth at 40°C and nitrate assimilation [4, 5].


goto top of outline Results

Neither fungi nor bacteria were cultured from single tracheal secretion; Haemophilus influenzae and other members of the mucosal microflora of the respiratory tract were isolated from nasal swabs. The results of oral swabs and sputum samples are summarised in table 2. There was no apparent correlation between age or sex of the patients and detection of specific pathogens. From most of the patients examined, P. aeruginosa and C. albicans were cultured, which is in accordance with the data from the literature [6].


Table 2. Bacteria and fungi isolated from 81 CF patients

The number of different bacterial and fungal species cultured from sputum samples was higher than that from oral swabs (table 2, fig. 1, 2). Figure 2 shows a more detailed analysis of the fungal isolates from sputum samples (SS) and oral swabs (OS) and compares them. Non-albicans Candida species were the only fungi, which were isolated at a higher frequency from oral swabs than from sputum samples. E. dermatitidis could not be cultured from any of the oral swabs. The fungi listed as ‘other hyphomycetes’ in table 2 and figure 2 did not develop any conidia that would have allowed microscopic identification. Because there were no clinical signs of infection in the patients from whom the samples were taken, no further attempts were made to identify these isolates.


Fig. 1. Comparison of bacterial groups and species isolates from oral swabs (OS) and sputum samples (SS). Numbers of complete isolates are given in brackets behind the name of the isolated micro-organism.


Fig. 2. Comparison of fungal groups and species isolates from oral swabs (OS) and sputum samples (SS). Number of complete isolates are given in brackets behind the name of the isolated fungus.

In figure 3, moulds detected using SGA are compared with those grown on ECA. Using ECA, mainly several isolates of Aspergillus and Penicillium spp. were obtained in addition to two strains of Pseudallescheria boydii. E. dermatitidis was cultured on SGA only once, but 5 times from 5 sputum samples when ECA was used. Colonies were recognised after 5–16 days of incubation, with an average time of about 10 days. In contrast to cultures incubated with SGA and CSA, no strains of P. aeruginosa grew on ECA, which may be a reason for the higher isolation rate using this selective medium. In general, during this study, E. dermatitidis was isolated from 1.1% of all specimens, from 1.6% of all sputum samples (table 2, fig. 2) and 6.2% from all patients examined (table 4).


Fig. 3. Comparison of results obtained using SGA and ECA for mould detection. Number of complete isolates are given in brackets behind the name of the isolated fungus.


Table 4. Summary of the isolation rate of E. dermatitidis from sputum samples from CF patients described in the literature

All 5 ECA samples positive for E. dermatitidis were from a single sample, although the number of samples analysed from the E. dermatitidis-positive patients ranged between 1 and 11. In 2 cases, family members also suffered from CF, but in these patients no black yeasts were observed. The data of the 5 E. dermatitidis-positive patients are compiled in table 3, including all isolates from each patient. The results of the samples, from which E. dermatitidis was cultured exhibit a greyish background. These patients’ ages ranged between 9 and 35 years (mean age, 21.8 years); male to female ratio was 1:4. Except patient 3, all patients had suffered from CF for more than 1 year and received glucocorticoids as well as antibiotics during this study. No special factor (e.g. antimicrobial treatment or time of CF diagnosis) was found to predispose to colonisation by E. dermatitidis. None of the 5 patients showed any signs of malnutrition. E. dermatitidis was invariably accompanied by P. aeruginosa, which grew on most media used, except for ECA. The presence of E. dermatitidis could not be related to any adverse effect on the health of any of the patients. During this study, none of our patients was hospitalised because of a fungal respiratory infection. However, 21 of the CF patients (25.9%) received in-hospital treatment due to other reasons. All of them showed more known risk factors for colonisation or infection due to E. dermatitidis, e.g. malnutrition or long-term antibiotic and/or antifungal treatment, than the 5 E. dermatitidis-positive patients, but E. dermatitidis was cultured from none of them.


Table 3. Data of the patients from whom E. dermatitidis was isolated

In table 4, the isolation rate of E. dermatitidis is compared with the results of other studies published.


goto top of outline Discussion

Facultative opportunistic pathogenic fungi (e.g. C. albicans and A. fumigatus) are often present in the respiratory tract and, in general, do not predispose to fungal infection. Those patients are at risk for fungal infection nearly exclusively when their immunity is reduced and/or the flora of the respiratory tract is disturbed. Infections due to C. albicans are mainly endogenous due to the patients’ own colonizing strains, while infections due to A. fumigatus are often exogenous as a result of inhaling the conidia, which are pervasive in the environment. In both cases, humans are permanently in contact with fungi. In infections due to ‘uncommon fungi’ such as E. dermatitidis, the situation is clearly different, because these fungi are not as widely present as C. albicans or A. fumigatus, either in the patient or in the environment. Besides the patient’s immunological status, the risk of infection may also depend on the kind of contact and the fungal mass. Until now, the risk of infection due to E. dermatitidis, especially in CF patients, is still a moot point. A total of 2,021 nosocomial infections by E. dermatitidis have been reported following intravascular [14] or intra-articular injection [15] of contaminated drugs. Some of these cases resulted in lethal outcomes [16].

Prior to the introduction of ECA, E. dermatitidis had never been isolated in our laboratory, either from CF, or from other patients. Although the detection of this fungus did not correlate with the clinical status of any of our patients, a medical significance of the organism cannot be completely excluded. Blaschke-Hellmessen et al. [8] observed clinical and radiological signs of pulmonary infections in a significant number of their patients. In another study, E. dermatitidis-specific antibodies were found in the sera of CF patients from whom this fungus had been isolated [17]. These data show that colonisation due to E. dermatitidis may result in pulmonary infections in humans, especially those suffering from CF. As other human opportunistic pathogens, e.g. Burgholderia cepacia, fungi such as E. dermatitidis are known to colonise the respiratory tract of CF patients and may lead to infection if additional predisposing factors are present [18]. Routine isolation techniques are insufficient to detect fungi in specimens from CF patients [18], especially, when P. aeruginosa is present in the sample, because pyocyanin and 1-hydroxyphenazine produced by these bacteria may inhibit fungal growth [19]. Therefore, additional diagnostic methods for the detection of fungi are necessary in CF patients. Bakare et al. [18] demonstrated that the amount of fungi isolated using antibiotic-containing culture media was much lower than that detected using fungal staining methods such as calcofluor white. However, in this study, the fungal culture media used were incubated and inspected only on 3 consecutive days [18], while prolonged incubation up to 4 weeks is more effective. With the ECA medium used in the study presented here, growth of E. dermatitidis and other fungi (table 2, fig. 23) needed 5 to nearly 20 days for detection.

E. dermatitidis can survive for many months in the environment, preferentially under a relative humidity between 49 and 70% [20]. It is known to occur in the direct vicinity of humans, e.g. in bathrooms [20] or in steam baths [21]. In contrast to Penicillium or Aspergillus spp., E. dermatitidis occurs nearly exclusively in a humid environment [20]. It is not a common airborne fungus. Therefore, airborne contamination of the ECA medium can be excluded. In the literature data compiled in table 4, the isolation rates of E. dermatitidis from sputum samples of CF patients ranged between 1.8 and 15.7%. Glucocorticoid treatment, antibiotic therapy and malnutrition are established risk factors for the colonisation of the respiratory tract by E. dermatitidis in CF patients [5]. Genetic factors may also play a role, as proposed for the colonisation by P. aeruginosa [22]. This would be in line with the observation that E. dermatitidis is a well-known causative agent of cerebral infections, preferentially in Asians [23], despite its environmental distribution being worldwide.

The opportunist P. boydii was encountered 3 times in our study, in all cases without associated clinical symptoms of disease. However, colonisation by this therapy-refractory species may imply a health risk for CF patients [24].

Surveillance of CF patients, from whom opportunistic pathogenic fungi have been isolated, will be necessary in order to start adequate therapy in case of respiratory infection as early as possible. In contrast, the situation is clearly different when the mainly saprophytic or plant pathogenic fungi Aureobasidium, Cladosporium and Microascus are isolated. Their pathogenicity is very low or questionable and human infections are mostly due to traumatic inoculation. In our routine practice, those isolates are kept frozen for up to about 6 months and discarded when no signs of infection can be recognised during this time and isolation cannot be repeated culturing another further specimen.

In case of the development of pulmonary fungal infections, the recognition of the occurrence of fungi, e.g. E. dermatitidis, by repeated isolation may predict infection by those fungi. During the last few years, bacterial detection in sputum samples from CF patients became increasingly cost-effective. Lots of selective isolation media are commercially available for the detection of ‘new’ pathogenic bacteria, e.g. Pseudomonas spp., B. cepacia, methicillin-resistant Staphylococcus aureus (MRSA). In contrast, less attention has been paid to the detection of fungi. Today, nearly exclusively fast-growing fungi like Candida,Aspergillus and Penicillium spp. will be detected with standard protocols [18].

Although lots of other human opportunistic pathogenic fungi have been described during the last decade [25, 26], their role in CF patients is controversial. More data are necessary on the occurrence and the pathogenicity of those ‘uncommon new pathogenic fungi’ in CF patients, and therefore, specialised selective isolation methods should be used and incubation of the media should be prolonged for up to 4 weeks.

 goto top of outline References
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 goto top of outline Author Contacts

Dr. med. Regine Horré
Federal Institute for Drugs and Medical Devices
Kurt-Georg-Kiesinger-Allee 3
DE–53175 Bonn (Germany)
Tel. +49 228 207 3267, E-Mail horre@bfarm.de

 goto top of outline Article Information

Received: May 21, 2003
Accepted after revision: January 29, 2004
Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 4, Number of References : 26

 goto top of outline Publication Details

Respiration (International Review of Thoracic Diseases)
Founded 1944 as ‘Schweizerische Zeitschrift für Tuberkulose und Pneumonologie’ by E. Bachmann, M. Gilbert, F. Häberlin, W. Löffler, P. Steiner and E. Uehlinger, continued 1962–1967 as ‘Medicina Thoracalis’ as of 1968 as ‘Respiration’, H. Herzog (1962–1997)
Official Journal of the European Association for Bronchology and Interventional Pulmonology

Vol. 71, No. 4, Year 2004 (Cover Date: July-August 2004)

Journal Editor: C.T. Bolliger, Cape Town
ISSN: 0025–7931 (print), 1423–0356 (Online)

For additional information: http://www.karger.com/journals/res

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