Journal Mobile Options
Table of Contents
Vol. 86, No. 4, 2004
Issue release date: November 2004
Section title: Original Paper
Biol Neonate 2004;86:247–253
(DOI:10.1159/000079907)

Benign B-Cell Precursors (Hematogones) Are the Predominant Lymphoid Population in the Bone Marrow of Preterm Infants

Rimsza L.M. · Douglas V.K. · Tighe P. · Saxonhouse M.A. · Calhoun D.A. · Christensen R.D. · Sola M.C.
aDepartment of Pathology, University of Arizona, Tucson, Ariz., bDepartment of Pathology, Immunology and Laboratory Medicine, cCollege of Medicine, dDepartment of Pediatrics, University of Florida, Gainesville, Fla. and eDepartment of Pediatrics, University of South Florida and All Children’s Hospital, St. Petersburg, Fla., USA

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restriction apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/27/2003
Accepted: 5/3/2004
Published online: 11/12/2004

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 3

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO

Abstract

Bone marrow (BM) findings in 3rd-trimester stillborns and full-term living neonates have been previously described. However, there is no information regarding BM composition in living preterm infants. Specifically, it is unknown whether the BM lymphocytosis seen in full-term infants at 1–4 weeks of age also occurs in preterm infants. Furthermore, the lineage of these cells has never been investigated. We used a panel of immunohistochemical stains to characterize the BM composition in 11 neonates (8 living and 3 deceased). Unlike in the other age groups, immature B cells (hematogones) were the most common lymphoid population, accounting for 10–60% (mean 34%) of all cells. In two additional cases (both living patients), flow cytometry revealed a level of 3.8% of immature B cells in a <1-week-old neonate and 25.7% in a 19-week-old infant. Immature B cells were not identified in 6 peripheral blood samples from preterm neonates. These findings are pertinent for the interpretation of BM and peripheral blood samples in this age group as survival improves and diagnostic samples become more common.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/27/2003
Accepted: 5/3/2004
Published online: 11/12/2004

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 3

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Sola MC, Rimsza LM, Christensen RD: A bone marrow biopsy technique suitable for use in neonates. Br J Haematol 1999;107:458–460.
  2. Calhoun DA, Christensen RD, Edstrom CS, Juul SE, Ohls RK, Schibler KR, Sola MC, Sullivan SE: Consistent approaches to procedures and practices in neonatal hematology. Clin Perinatol 2000;27:733–753.
  3. Rosse C, Kraemer MJ, Dillon TL, McFarland R, Smith NJ: Bone marrow cell populations of normal infants; the predominance of lymphocytes. J Lab Clin Med 1977;89:1225–1240.
  4. McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry. Blood 2001;98:2498–2507.
  5. Rimsza LM, Larson RS, Winter SS, Foucar K, Chong YY, Garner KW, Leith CP: Benign hematogone-rich lymphoid proliferations can be distinguished from B-lineage acute lymphoblastic leukemia by integration of morphology, immunophenotype, adhesion molecule expression, and architectural features. Am J Clin Pathol 2000;114:66–75.
  6. Wells DA, Sale GE, Shulman HM, Myerson D, Bryant EM, Gooley T, Loken MR: Multidimensional flow cytometry of marrow can differentiate leukemic from normal lymphoblasts and myeloblasts after chemotherapy and bone marrow transplantation. Am J Clin Pathol 1998;110:84–94.
  7. Longacre TA, Foucar K, Crago S, Chen IM, Griffith B, Dressler L, McConnell TS, Duncan M, Gribble J: Hematogones: A multiparameter analysis of bone marrow precursor cells. Blood 1989;73:543–552.
  8. Kalpaktsoglou PK, Emery JL: Human bone marrow during the last three months of intrauterine life. A histological study. Acta Haematol 1965;34:228–238.
  9. Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: A novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995;86:1453–1459.
  10. Moscatello KM, Biber KL, Dempsey DC, Chervenak R, Wolcott RM: Characterization of a B cell progenitor present in neonatal bone marrow and spleen but not in adult bone marrow and spleen. J Immunol 1998;161:5391–5398.
  11. Cates KL, Goetz C, Rosenberg N, Pantschenko A, Rowe JC, Ballow M: Longitudinal development of specific and functional antibody in very low birth weight premature infants. Pediatr Res 1988;23:14–22.
  12. Ballow M, Cates KL, Rowe JC, Goetz C, Desbonnet C: Development of the immune system in very low birth weight (less than 1,500 g) premature infants: Concentrations of plasma immunoglobulins and patterns of infections. Pediatr Res 1986;20:899–904.
  13. Comans-Bitter WM, de Groot R, van den Beemd R, Neijens HJ, Hop WC, Groeneyeld K, Hooijkaas, H, van Dongen JJ: Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr 1997;130:388–393.
  14. de Vries E, de Bruin-Versteeg S, Comans-Bitter WM, de Groot R, Hop WC, Boerma GJ, Lotgering FK, Van Dongen JJ: Longitudinal survey of lymphocyte subpopulations in the first year of life. Pediatr Res 2000;47:528–537.
  15. Brady KA, Atwater SK, Lowell CA: Flow cytometric detection of CD10 (cALLA) on peripheral blood B lymphocytes of neonates. Br J Haematol 1999;107:712–715.