Journal Mobile Options
Table of Contents
Vol. 135, No. 1, 2004
Issue release date: September 2004
Int Arch Allergy Immunol 2004;135:3–11
(DOI:10.1159/000080036)

Proteomic Analysis of Putative Latex Allergens

Yagami T. · Haishima Y. · Tsuchiya T. · Tomitaka-Yagami A. · Kano H. · Matsunaga K.
aDivision of Medical Devices, National Institute of Health Sciences, Tokyo, bDepartment of Dermatology, Fujita Health University School of Medicine, Toyoake, Japan

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

Background: Extensive analysis of allergenic proteins is generally time-consuming and labor-intensive. Accordingly, a rapid and easy procedure for allergen identification is required. As sequence information on proteins and genes is accumulated in databases, it is becoming easier to identify a candidate protein using proteomic strategies, i.e. two-dimensional gel electrophoresis, site-specific fragmentation, mass spectrometry and then database search. In this study, we evaluated the usefulness of a proteomic strategy for identifying putative allergens through its application to latex proteins. Methods: Latex proteins were separated with two-dimensional gel electrophoresis, and putative allergens were visualized by IgE immunoblotting using pooled serum from latex-sensitive patients. The IgE-interactive proteins were cut out from the negatively stained two-dimensional gel and subjected to in-gel digestion by trypsin. Then the resulting peptides were analyzed with mass spectrometry. Based on the mass spectrometric data we obtained, the allergen candidates were assigned by a database search. Results: Five previously reported allergens and five new allergen candidates were identified with the proteomic approach without isolating the individual proteins. Less than 1 mg of crude latex protein was sufficient for the entire protocol. Because plural proteins can be processed in parallel, analysis of about 50 IgE-interactive proteins was accomplished within 1 week. Conclusions: Analysis of putative allergens with proteomic strategies (allergenomics) is a promising avenue for rapid and exhaustive research. The high resolving power of two-dimensional gel electrophoresis is superior to conventional gel electrophoresis. Moreover, the notable sensitivity and speed of mass spectrometry have pronounced advantages over the N-terminal sequencing that has generally been used for protein identification.



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Ebo DG, Stevens WJ: IgE-mediated natural rubber latex allergy: An update. Acta Clin Belg 2002;57:58–70.
  2. Yagami T, Sato M, Nakamura A, Komiyama T, Kitagawa K, Akasawa A, Ikezawa Z: Plant defense-related enzymes as latex antigens. J Allergy Clin Immunol 1998;101:379–385.
  3. Yagami T: Plant defense-related proteins and latex allergy. Environ Dermatol 1998;5(suppl 2):31–39.
  4. Yagami T: Defense-related proteins as families of cross-reactive plant allergens. Recent Res Dev Allergy Clin Immunol 2000;1:41–64.
  5. Yagami T: Allergies to cross-reactive plant proteins: Latex-fruit syndrome is comparable with pollen-food allergy syndrome. Int Arch Allergy Immunol 2002;128:271–279.
  6. Yagami T: Features and mode of action of cross-reactive plant allergens relevant to latex-fruit syndrome. Food Agric Immunol 2002;14:241–253.
  7. Wagner S, Breiteneder H: The latex-fruit syndrome. Biochem Soc Trans 2002;30:935–940.
  8. Blanco C: Latex-fruit syndrome. Curr Allergy Asthma Rep 2003;3:47–53.
  9. Peng J, Gygi SP: Proteomics: The move to mixtures. J Mass Spectrom 2001;36:1083–1091.
  10. Tichá M, Pacáková V, Štulík K: Proteomics of allergens. J Chromatogr B Analyt Technol Biomed Life Sci 2002;771:343–353.
  11. Raftery MJ, Saldanha RG, Geczy CL, Kumar RK: Mass spectrometric analysis of electrophoretically separated allergens and proteases in grass pollen diffusates. Respir Res 2003;4:10.
  12. Petersen A: Two-dimensional electrophoresis replica blotting: A valuable technique for the immunological and biochemical characterization of single components of complex extracts. Proteomics 2003;3:1206–1214.
  13. Dreborg S: Skin tests in the diagnosis of food allergy. Pediatr Allergy Immunol 1995;6(suppl 8):38–43.

    External Resources

  14. Katayama H, Satoh K, Takeuchi M, Deguchi-Tawarada M, Oda Y, Nagasu T: Optimization of in-gel protein digestion system in combination with thin-gel separation and negative staining in 96-well plate format. Rapid Commun Mass Spectrom 2003;17:1071–1078.
  15. Katayama H, Nagasu T, Oda Y: Improvement of in-gel digestion protocol for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2001;15:1416–1421.
  16. Medzihradszky KF, Campbell JM, Baldwin MA, Falick AM, Juhasz P, Vestal ML, Burlingame AL: The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer. Anal Chem 2000;72:552–558.
  17. Yagami T, Osuna H, Kouno M, Haishima Y, Nakamura A, Ikezawa Z: Significance of carbohydrate epitopes in a latex allergen with β-1,3-glucanase activity. Int Arch Allergy Immunol 2002;129:27–37.
  18. Van Ree R: Carbohydrate epitopes and their relevance for the diagnosis and treatment of allergic diseases. Int Arch Allergy Immunol 2002;129:189–197.
  19. Kurup VP, Alenius H, Kelly KJ, Castillo L, Fink JN: A two-dimensional electrophoretic analysis of latex peptides reacting with IgE and IgG antibodies from patients with latex allergy. Int Arch Allergy Immunol 1996;109:58–67.
  20. Posch A, Chen Z, Wheeler C, Dunn MJ, Raulf-Heimsoth M, Baur X: Characterization and identification of latex allergens by two-dimensional electrophoresis and protein microsequencing. J Allergy Clin Immunol 1997;99:385–395.
  21. Duong PT, Chang FN: A simple method for assigning multiple immunogens to their protein on a two-dimensional blot and its application to asthma-causing allergens. Electrophoresis 2001;22:2098–2102.
  22. Beyer K, Bardina L, Grishina G, Sampson HA: Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens. J Allergy Clin Immunol 2002;110:154–159.
  23. Beyer K, Grishina G, Bardina L, Grishin A, Sampson HA: Identification of an 11S globulin as a major hazelnut food allergen in hazelnut-induced systemic reactions. J Allergy Clin Immunol 2002;110:517–523.
  24. Yu CJ, Lin YF, Chiang BL, Chow LP: Proteomics and immunological analysis of a novel shrimp allergen, Pen m 2. J Immunol 2003;170:445–453.
  25. Russell WK, Park ZY, Russell DH: Proteolysis in mixed organic-aqueous solvent systems: Applications for peptide mass mapping using mass spectrometry. Anal Chem 2001;73:2682–2685.


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50