Journal Mobile Options
Table of Contents
Vol. 177, No. 4, 2004
Issue release date: 2004
Section title: Original Paper
Cells Tissues Organs 2004;177:221–228
(DOI:10.1159/000080135)

Expression of Wee1 and Its Related Cell Cycle Components in Mouse Early Stage Follicles

Park C.-E. · Kim Y.-H. · Jeon E.-H. · Cha K.-Y. · Lee S.-H. · Lee K.-A.
aInfertility Medical Center, CHA General Hospital, bGraduate School of Life Science and Biotechnology, Pochon CHA University, and cGenome Research Center for Reproductive Medicine and Infertility, CHA General Hospital, Seoul, Korea

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restriction apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 5/12/2004
Published online: 9/30/2004

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 2

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Wee1 is a kinase regulator of the M-phase promoting factor (a complex of cdc2 and cyclin B1). The present study was performed to determine the role(s) of wee1 in the early stages of mouse ovarian follicles. Expression of wee1 and the correlated cell cycle components, namely cdc2, cyclin B1, and cdc25C, was evaluated by immunohistochemistry. In addition, expression of Tyr15-phosphorylated cdc2 (cdc2-p) was also examined to determine whether wee1 kinase phosphorylates cdc2. Each component except cdc25C was found in the oocyte cytoplasm at all follicular stages, while cdc25C was not detected in primordial follicles. It was found primarily in ovarian interstitial cells and to a small extent in granulosa cells of the developing secondary follicles. To further confirm the expression of cell cycle components in the primordial follicular oocytes, day 1 ovaries were enzymatically and mechanically dissociated, then oocytes were isolated from somatic cells including pre-granulosa cells, and we confirmed that cdc2-p was expressed in oocytes of primordial follicles. The results of the present study led to the conclusion that wee1, without the counteracting cdc25C, would cause meiotic arrest of oocytes by inhibitory phosphorylation of cdc2. Expression of all these proteins in the granulosa cells of growing follicles may regulate granulosa cell mitosis concurrently with the growth of oocytes and follicles.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 5/12/2004
Published online: 9/30/2004

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 2

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Chesnel, F., J.J. Eppig (1995) Induction of precocious germinal vesicle breakdown (GVB) by GVB-incompetent mouse oocytes: possible role of mitogen-activated protein kinases rather than p34cdc2 kinase. Biol Reprod 52: 895–902.
  2. Choi, T., F. Aoki, M. Mori, M. Yamashita, Y. Nagahama, K. Kohmoto (1991) Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos. Development 113: 789–795.
  3. Eppig, J.J., K. Wigglesworth (2000) Development of mouse and rat oocytes in chimeric reaggregated ovaries after interspecific exchange of somatic and germ cell components. Biol Reprod 63: 1014–1023.
  4. Hashimoto, N., T. Kishimoto (1988) Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation. Dev Biol 126: 242–252.
  5. Hunt, T. (1991) Cyclins and their partners: from a simple idea to complicated reality. Semin Cell Biol 2: 213–222.
  6. Kanatsu-Shinohara, M., R.M. Schultz, G.S. Kopf (2000) Acquisition of meiotic competence in mouse oocytes: absolute amounts of p34(cdc2), cyclin B1, cdc25C, and wee1 in meiotically incompetent and competent oocytes. Biol Reprod 63: 1610–1616.
  7. Millar, J.B., P. Russell (1992) The cdc25 M-phase inducer: an unconventional protein phosphatase. Cell 68: 407–410.
  8. Mitra, J., R.M. Schultz (1996) Regulation of the acquisition of meiotic competence in the mouse: changes in the subcellular localization of cdc2, cyclin B1, cdc25C and wee1, and in the concentration of these proteins and their transcripts. J Cell Sci 109: 2407–2415.
  9. Mueller, P.R., T.R. Coleman, W.G. Dunphy (1995) Cell cycle regulation of a Xenopus Wee1-like kinase. Mol Biol Cell 6: 119–134.
  10. Nurse, P. (1997) Regulation of the eukaryotic cell cycle. Eur J Cancer 33: 1002–1004.
  11. Park, C.E., J.J. Ko, S.H. Lee, K.Y. Cha, K. Kim, K.A. Lee (2002) Analysis of the gene expression by laser capture microdissection (II): Differential gene expression between primordial and primary follicles. Dev Reprod 6: 89–96.
  12. Pines, J., T. Hunter (1991) Human cyclin A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. J Cell Biol 115: 11–17.
  13. de Vantery, C., A. Stutz, J.D. Vassalli, S. Schorderet-Slatkine (1997) Acquisition of meiotic competence in growing mouse oocytes is controlled at both translational and posttranslational levels. Dev Biol 187: 43–54.
  14. Wolgemuth, D.J., E. Laurion, K.M. Lele (2002) Regulation of the mitotic and meiotic cell cycles in the male germ line. Recent Prog Horm Res 57: 75–101.
  15. Yang, J., S. Kornbluth (1999) All aboard the cyclin train: Subcellular trafficking of cyclins and their CDK partners. Trends Cell Biol 9: 207–210.