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Vol. 135, No. 1, 2004
Issue release date: September 2004

Association of Single Nucleotide Polymorphisms in the Eosinophil Peroxidase Gene with Japanese Cedar Pollinosis

Nakamura H. · Higashikawa F. · Miyagawa K. · Nobukuni Y. · Endo T. · Imai T. · Ozasa K. · Motohashi Y. · Matsuzaki I. · Sasahara S. · Hatta K. · Ogino K. · Eboshida A.
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Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

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