Journal Mobile Options
Table of Contents
Vol. 108, No. 1-3, 2005
Issue release date: 2005
Cytogenet Genome Res 108:191–196 (2005)
(DOI:10.1159/000080815)

Investigation of marmoset hybrids (Cebuella pygmaea × Callithrix jacchus) and related Callitrichinae (Platyrrhini) by cross-species chromosome painting and comparative genomic hybridization

Neusser M. · Münch M. · Anzenberger G. · Müller S.
To view the fulltext, log in and/or choose pay-per-view option

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

We report on the cytogenetics of twin offspring from an interspecies cross in marmosets (Callitrichinae, Platyrrhini), resulting from a pairing between a female Common marmoset (Callithrix jacchus, 2n = 46) and a male Pygmy marmoset (Cebuella pygmaea, 2n = 44). We analyzed their karyotypes by multi-directional chromosome painting employing human, Saguinus oedipus and Lagothrix lagothricha chromosome-specific probes. Both hybrid individuals had a karyotype with a diploid chromosome number of 2n = 45. As a complementary tool, interspecies comparative genomic hybridization (iCGH) was performed in order to screen for genomic imbalances between the hybrids and their parental species, and between Callithrix argentata and S. oedipus, respectively. These genomic imbalances were confined to centromeric and telomeric heterochromatin, while euchromatic chromosome regions appeared balanced in all species investigated. When comparing marmosets and tamarins, sequence divergence of centromeric heterochromatin was already clearly noticeable. In the C. argentata and C. pygmaea genomes numerous subtelomeric regions were affected by amplification of different repetitive sequences. Cross-species FISH with a microdissection-derived C. pygmaea repetitive probe revealed species specificity of this repetitive sequence at the molecular cytogenetic level of resolution.   



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Alves G, Canavez F, Seuánez HN, Fanning T: Recently amplified satellite DNA in Callithrix argentata (Primates, Platyrrhini). Chromosome Res 3:207–213 (1995).

    External Resources

  2. Anzenberger G, Münch M, Moisson P, Petit C: Intergeneric marmoset hybrids (Cebuella pygmaea × Callithrix jacchus): An important case study for callitrichid taxonomy. Folia Primatol 72:153 (2001).

    External Resources

  3. Barroso CML, Schneider H, Schneider MPC, Sampaio I, Harada ML, Czelusniak J, Goodman M: Update on the phylogenetic systematics of New World monkeys: Further DNA evidence for placing the pygmy marmoset (Cebuella) within the genus Callithrix. Int J Primatol 18:651–674 (1997).

    External Resources

  4. Canavez FC, Alves G, Fanning TG, Seuánez HN: Comparative karyology and evolution of the Amazonian Callithrix (Platyrrhini, Primates). Chromosoma 104:348–357 (1996).

    External Resources

  5. Canavez FC, Moreira MA, Simon F, Parham P, Seuánez HN: Phylogenetic relationships of the Callitrichinae (Platyrrhini, Primates) based on beta2-microglobulin DNA sequences. Am J Primatol 48:225–236 (1999).

    External Resources

  6. Coimbra-Filho AF, Pissinatti A, Rylands AB: Experimental multiple hybridism among Callithrix species from eastern Brasil, in Rylands AB (ed): Marmosets and Tamarins: Systematics, Ecology and Behaviour, pp 95–120 (Oxford University Press, Oxford 1993).
  7. Dugoujon JM, Moro F, Larrouy G: Cytogenetic study of Cercopithecus pogonias grayi × Cercopithecus ascanius katangae hybrids. Folia Primatol 38:269–274 (1982).
  8. King M: Species Evolution. The Role of Chromosome Change (Cambridge University Press, Cambridge 1993).
  9. Moore CM, Harris CP, Abee CR: Distribution of chromosomal polymorphisms in three subspecies of squirrel monkeys (genus Saimiri). Cytogenet Cell Genet 53:118–122 (1990).

    External Resources

  10. Moore CM, Janish C, Eddy CA, Hubbard GB, Leland MM, Rogers J: Cytogenetic and fertility studies of a rheboon, rhesus macaque (Macaca mulatta) × baboon (Papio hamadryas) cross: further support for a single karyotype nomenclature. Am J Phys Anthropol 110:119–127 (1999).

    External Resources

  11. Müller S, Neusser M, Stanyon R, Wienberg J: Molecular cytogenetic characterization of EBV producing cell line B95-8 (S. oedipus, Platyrrhini) by flow cytometry and multicolour cross species chromosome painting. Chromosome Res 9:689–693 (2001).
  12. Müller S, Neusser M, Wienberg J: Towards unlimited colors for fluorescence in situ hybridization (FISH). Chromosome Res 10:223–232 (2002).

    External Resources

  13. Nagamachi CY, Pieczarka JC, Barros RM: Karyotypic comparison among Cebuella pygmaea, Callithrix jacchus and C. emiliae (Callitrichidae, Primates) and its taxonomic implications. Genetica 85:249–257 (1992).

    External Resources

  14. Natori M: Craniometrical variations among eastern Brazilian marmosets and their relationships. Primates 35:167–176 (1994).

    External Resources

  15. Neusser M, Wienberg J, Stanyon, R, Müller S: Molecular cytotaxonomy of Platyrrhini – comparative analysis of five species by multicolour reciprocal chromosome painting gives evidence for a classification of Callimico goeldii with Callitrichidae. Cytogenet Cell Genet 94:206–215 (2001).
  16. Pellicciari C, Formenti D, Zuccotti M, Stanyon R, Manfredi Romanini MG: Genome size and constitutive heterochromatin in Hylobates muelleri and Symphalangus syndactylus and in their viable hybrid. Cytogenet Cell Genet 47:1–4 (1988).

    External Resources

  17. Porter CA, Page SL, Czelusniak J, Schneider H, Schneider MPC, Sampaio I, Goodman M: Sequences of the primate epsilon-globin gene: implications for systematics of the marmosets and other New World primates. Gene 205:59–71 (1997).
  18. Ratomponirina C, Andrianivo J, Rumpler Y: Spermatogenesis in several intra- and interspecific hybrids of the lemur (Lemur). J Reprod Fertil 66:717–721 (1982).
  19. Roberts I, Wienberg J, Nacheva E, Grace C, Griffin D, Coleman N: Novel method for the production of multiple colour chromosome paints for use in karyotyping by fluorescence in situ hybridisation. Genes Chromosomes Cancer 25:241–250 (1999).

    External Resources

  20. Rosenberger AL, Coimbra-Filho AF: Morphology, taxonomic status of the lion tamarins Leontopithecus (Callithrichinae, Cebidae). Folia Primatol 42:149–179 (1984).

    External Resources

  21. Rumpler Y, Dutrillaux B: Chromosomal evolution in Malagasy lemurs. I. Chromosome banding studies in the genuses Lemur and Microcebus. Cytogenet Cell Genet 17:268–281 (1976).

    External Resources

  22. Rylands AB, Schneider H, Langguth A, Mittermeier RA, Groves CP, Rodriguez-Luna E: An assessment of the diversity of New World primates. Neotropical Primates 8:61–93 (2000).
  23. Schwarzacher T, Heslop-Harrison P: Practical in situ Hybridization (Bios Scientific Publishers Ltd, Oxford 2000).
  24. Stanyon R, Consigliere S, Bigoni F, Ferguson-Smith M, O’Brien PC, Wienberg J: Reciprocal chromosome painting between a New World primate, the woolly monkey, and humans. Chromosome Res 9:97–106 (2001).
  25. Toder R, Xia Y, Bausch E: Interspecies comparative genome hybridization and interspecies representational difference analysis reveal gross DNA differences between humans and great apes. Chromosome Res 6:487–494 (1998).

    External Resources

  26. Weimer J, Kiechle M, Arnold N: FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements. Cytogenet Cell Genet 88:114–118 (2000).

    External Resources

  27. Yunis E, De Caballero OM, Ramirez C: Genus Aotus Q- and G-band karyotypes and natural hybrids. Folia Primatol 27:165–177 (1977).

    External Resources



Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50