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Vol. 12, No. 3, 2005
Issue release date: May 2005
Section title: Original Paper
Neuroimmunomodulation 2005;12:157–163
(DOI:10.1159/000084848)

Human Endometrial Leukemia Inhibitory Factor and Interleukin-6: Control of Secretion by Transforming Growth Factor-β-Related Members

Perrier d’Hauterive S. · Charlet-Renard C. · Dubois M. · Berndt S. · Goffin F. · Foidart J.-M. · Geenen V.
aUniversity of Liège, Department of Medicine, Center of Immunology, Institute of Pathology CHU-B23, Liège-Sart Tilman, bUniversity of Liège, Department of Gynecology and Obstetrics, CHR-Citadelle, Liège, and cUniversity of Liège, Institute of Pathology CHU-B23, Laboratory of Tumor and Developmental Biology, Liège-Sart Tilman, Belgium

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/16/2003
Accepted: 8/11/2004
Published online: 5/17/2005

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM

Abstract

Objective(s): The implantation process is closely linked to the fundamental question of the tolerance of the maternal immune system. The main objective of this study was to investigate whether different members of the transforming growth factor-β (TGF-β) superfamily could intervene in the first steps of embryo implantation by modulating the secretion of proimplantatory leukemia inhibitory factor (LIF) and in the tolerance of the fetal graft by regulating proinflammatory interleukin (IL)-6 secretion by human endometrial epithelium (EEC) in vitro. Methods: EEC were isolated from biopsies collected from 16 informed and consenting fertile women and were cultured for 72 h. Cytokine measurements (LIF and IL-6) were realized by ELISA. Results: TGF-β1 (from 10–12 to 10–8M), -β2, -β3 and activin A (10–10 and 10–8M) increased LIF secretion by EEC cultures. Inhibin B (10–10 and 10–8M) did not stimulate LIF production by human EEC. Contrastingly, TGF-β1 (from 10–12 to 10–8M), -β2, -β3 and activin A (10–10 and 10–8M) reduced IL-6 release by the same cells. Activin A at 10–8 M also significantly reduced the stimulating effect of IL-1β (10–9M) which is known to stimulate LIF production by EEC. Only the highest concentration of inhibin B (10–8M) reduced IL-6 secretion by EEC, but did not modulate IL-1β-induced stimulation of IL-6 secretion. Conclusion(s): Besides their role in the control of the process of implantation and in the induction of embryonic mesoderm, different members of the TGF-β superfamily may also contribute in the reproductive process by enhancing endometrial proimplantatory LIF secretion and reducing proinflammatory IL-6 release by EEC.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/16/2003
Accepted: 8/11/2004
Published online: 5/17/2005

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM


Copyright / Drug Dosage

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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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