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Vol. 27, No. 2-4, 2005
Issue release date: March–August 2005
Section title: Original Paper
Dev Neurosci 2005;27:127–133
(DOI:10.1159/000085984)

Strain Variability, Injury Distribution, and Seizure Onset in a Mouse Model of Stroke in the Immature Brain

Comi A.M. · Johnston M.V. · Wilson M.A.
Departments of aNeurology, bPediatrics, and cNeuroscience, Johns Hopkins University School of Medicine, and eKennedy Krieger Research Institute, Baltimore, Md., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/16/2004
Accepted: 12/19/2004
Published online: 7/19/2005

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE

Abstract

Neonatal stroke is an important cause of neurologic morbidity and cerebral palsy. Recently, we have determined that in postnatal day 12 CD1 mice unilateral carotid ligation alone results in seizures and brain injury. We have shown that, in this model, seizure scores correlate with brain injury scores. We have applied this model to another strain of mice to assess strain-related differences in vulnerability to seizures and brain injury after unilateral carotid ligation. Under isoflurane anesthesia, unilateral right-sided carotid ligation was performed in postnatal day 12 C3HeB/FeJ mice followed by a 4-hour period of observation in a 35°C incubator. Seizure scores and brain jury scores were assigned and compared to scores in mice receiving sham surgery. Timing of seizure onset and regional distribution of brain injury were compared in the CD1 and C3HeB/FeJ mice. Unilateral carotid ligation in postnatal day 12 C3HeB/FeJ mice resulted in seizure behavior and brain injury in some animals, with similar time to seizure onset and regional injury distribution, but affected a significantly smaller percentage of C3HeB/FeJ pups than that observed in postnatal day 12 CD1 mice, indicating strain-related vulnerability in this model.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/16/2004
Accepted: 12/19/2004
Published online: 7/19/2005

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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