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Table of Contents
Vol. 27, No. 1, 2006
Issue release date: December 2005
Section title: Research Article
Free Access
Tumor Biol 2006;27:17–26
(DOI:10.1159/000090152)

Silencing the hsp25 Gene Eliminates Migration Capability of the Highly Metastatic Murine 4T1 Breast Adenocarcinoma Cell

Bausero M.A.a, c · Bharti A.a · Page D.T.a · Perez K.D.a, b · Eng J.W.-L.a · Ordonez S.L.a · Asea E.E.a, b · Jantschitsch C.d · Kindas-Muegge I.e · Ciocca D.f · Asea A.a, b
aCenter for Molecular Stress Response, Boston University Medical Center and Boston University School of Medicine, Boston, Mass., bDivision of Investigative Pathology, Department of Pathology, Scott & White Clinic and Texas A&M University System Health Science Center College of Medicine, Temple, Tex., USA; cLaboratorio de Oncología Básica y Biología Molecular, Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; dDivision of Special and Environmental Dermatology, Department of Dermatology, University of Vienna, eInstitute of Cancer Research, Vienna, Austria; fInstitute of Experimental Medicine and Biology of Cuyo (IMBECU)-CONICET, Mendoza, Argentina
email Corresponding Author

Dr. Alexzander Asea, Division of Investigative Pathology

Scott & White Clinic and The Texas A&M University System Health Science Center College of Medicine, 2401 South 31st Street

Temple, TX 76508 (USA)

Tel. +1 254 743 0201, Fax +1 254 743 0247, E-Mail asea@medicine.tamhsc.edu


References

  1. Weir HK, Thun MJ, Hankey BF, Ries LA, Howe HL, Wingo PA, Jemal A, Ward E, Anderson RN, Edwards BK: Annual report to the nation on the status of cancer, 1975–2000, featuring the uses of surveillance data for cancer prevention and control. J Natl Cancer Inst 2003;95:1276–1299.
  2. Kim J, Yu W, Kovalski K, Ossowski L: Requirement for specific proteases in cancer cell intravasation as revealed by a novel semiquantitative PCR-based assay. Cell 1998;94:353–362.
  3. Egeblad M, Werb Z: New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer 2002;2:161–174.
  4. Neumark E, Sagi-Assif O, Shalmon B, Ben-Baruch A, Witz IP: Progression of mouse mammary tumors: MCP-1-TNFalpha cross-regulatory pathway and clonal expression of promalignancy and antimalignancy factors. Int J Cancer 2003;106:879–886.
  5. Lirdprapamongkol K, Sakurai H, Kawasaki N, Choo MK, Saitoh Y, Aozuka Y, Singhirunnusorn P, Ruchirawat S, Svasti J, Saiki I: Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells. Eur J Pharm Sci 2005;25:57–65.
  6. Soldes OS, Kuick RD, Thompson IA 2nd, Hughes SJ, Orringer MB, Iannettoni MD, Hanash SM, Beer DG: Differential expression of Hsp27 in normal oesophagus, Barrett’s metaplasia and oesophageal adenocarcinomas. Br J Cancer 1999;79:595–603.
  7. Jantschitsch C, Trautinger F: Heat shock and UV-B-induced DNA damage and mutagenesis in skin. Photochem Photobiol Sci 2003;2:899–903.
  8. Wieder R: Insurgent micrometastases: sleeper cells and harboring the enemy. J Surg Oncol 2005;89:207–210.
  9. Oesterreich S, Hilsenbeck SG, Ciocca DR, Allred DC, Clark GM, Chamness GC, Osborne CK, Fuqua SA: The small heat shock protein HSP27 is not an independent prognostic marker in axillary lymph node-negative breast cancer patients. Clin Cancer Res 1996;2:1199–1206.
  10. Ciocca DR, Vargas-Roig LM: Hsp27 as a prognostic and predictive factor in cancer. Prog Mol Subcell Biol 2002;28:205–218.
  11. Ciocca DR, Green S, Elledge RM, Clark GM, Pugh R, Ravdin P, Lew D, Martino S, Osborne CK: Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study). Clin Cancer Res 1998;4:1263–1266.
  12. Ciocca DR, Rozados VR, Cuello Carrion FD, Gervasoni SI, Matar P, Scharovsky OG: Hsp25 and Hsp70 in rodent tumors treated with doxorubicin and lovastatin. Cell Stress Chaperones 2003;8:26–36.
  13. Cornford PA, Dodson AR, Parsons KF, Desmond AD, Woolfenden A, Fordham M, Neoptolemos JP, Ke Y, Foster CS: Heat shock protein expression independently predicts clinical outcome in prostate cancer. Cancer Res 2000;60:7099–7105.
  14. Bausero MA, Page DT, Osinaga E, Asea A: Surface expression of Hsp25 and Hsp72 differentially regulates tumor growth and metastasis. Tumor Biol 2004;25:243–251.
  15. Asea A: Exogenous Hsp70: principles and application of the chaperokine activity of Hsp70; in Henderson B, Pockley AG (eds): The Extracellular Biology of Molecular Chaperones. London, Cambridge University Press, 2005.
  16. Asea A, Ara G, Teicher BA, Stevenson MA, Calderwood SK: Effects of the flavonoid drug quercetin on the response of human prostate tumours to hyperthermia in vitro and in vivo. Int J Hyperthermia 2001;17:347–356.
  17. Jaattela M, Wissing D, Kokholm K, Kallunki T, Egeblad M: Hsp70 exerts its anti-apoptotic function downstream of caspase-3-like proteases. EMBO J 1998;17:6124–6134.
  18. Calderwood SK, Asea A: Targeting HSP70-induced thermotolerance for design of thermal sensitizers. Int J Hyperthermia 2002;18:597–608.
  19. Jaattela M: Programmed cell death: many ways for cells to die decently. Ann Med 2002;34:480–488.
  20. Farkas B, Hantschel M, Magyarlaki M, Becker B, Scherer K, Landthaler M, Pfister K, Gehrmann M, Gross C, Mackensen A, Multhoff G: Heat shock protein 70 membrane expression and melanoma-associated marker phenotype in primary and metastatic melanoma. Melanoma Res 2003;13:147–152.
  21. Asea A, Rehli M, Kabingu E, Boch JA, Bare O, Auron PE, Stevenson MA, Calderwood SK: Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4. J Biol Chem 2002;277:15028–15034.
  22. Asea A, Kraeft SK, Kurt-Jones EA, Stevenson MA, Chen LB, Finberg RW, Koo GC, Calderwood SK: HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine. Nat Med 2000;6:435–442.
  23. Asea A, Kabingu E, Stevenson MA, Calderwood SK: HSP70 peptide-bearing and peptide-negative preparations function as chaperokines. Cell Stress Chaperones 2000;5:425–431.
  24. Basu S, Suto R, Binder RJ, Srivastava PK: Heat shock proteins as novel mediators of cytokine secretion by macrophages. Cell Stress Chaperones 1998;3:11–16.
  25. Blackburn RV, Galoforo SS, Berns CM, Armour EP, McEachern D, Corry PM, Lee YJ: Comparison of tumor growth between hsp25- and hsp27-transfected murine L929 cells in nude mice. Int J Cancer 1997;72:871–877.
  26. Lemieux P, Oesterreich S, Lawrence JA, Steeg PS, Hilsenbeck SG, Harvey JM, Fuqua SA: The small heat shock protein hsp27 increases invasiveness but decreases motility of breast cancer cells. Invasion Metastasis 1997;17:113–123.
  27. Eskenazi AE, Powers J, Pinkas J, Oesterreich S, Fuqua SA, Frantz CN: Induction of heat shock protein 27 by hydroxyurea and its relationship to experimental metastasis. Clin Exp Metastasis 1998;16:283–290.
  28. Aldrian S, Trautinger F, Frohlich I, Berger W, Micksche M, Kindas-Mugge I: Overexpression of Hsp27 affects the metastatic phenotype of human melanoma cells in vitro. Cell Stress Chaperones 2002;7:177–185.
  29. Park SH, Lee YS, Osawa Y, Hachiya M, Akashi M: Hsp25 regulates the expression of p21(Waf1/Cip1/Sdi1) through multiple mechanisms. J Biochem (Tokyo) 2002;131:869–875.
  30. Lee YJ, Cho HN, Jeoung DI, Soh JW, Cho CK, Bae S, Chung HY, Lee SJ, Lee YS: HSP25 overexpression attenuates oxidative stress-induced apoptosis: roles of ERK1/2 signaling and manganese superoxide dismutase. Free Radic Biol Med 2004;36:429–444.
  31. Smith MC, Luker KE, Garbow JR, Prior JL, Jackson E, Piwnica-Worms D, Luker GD: CXCR4 regulates growth of both primary and metastatic breast cancer. Cancer Res 2004;64:8604–8612.
  32. Liang Z, Yoon Y, Votaw J, Goodman MM, Williams L, Shim H: Silencing of CXCR4 blocks breast cancer metastasis. Cancer Res 2005;65:967–971.
  33. Wang T, Yamashita K, Iwata K, Hayakawa T: Both tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2 activate Ras but through different pathways. Biochem Biophys Res Commun 2002;296:201–205.
  34. Tanimura S, Asato K, Fujishiro SH, Kohno M: Specific blockade of the ERK pathway inhibits the invasiveness of tumor cells: down-regulation of matrix metalloproteinase-3/-9/-14 and CD44. Biochem Biophys Res Commun 2003;304:801–806.