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Table of Contents
Vol. 24, No. 2, 2006
Issue release date: February 2006
Section title: Original Paper
Blood Purif 2006;24:236–246
(DOI:10.1159/000091028)

uPAR (CD87) as a Biocompatibility Marker of Dialysis Membrane

Sohka T. · Ohno T. · Ichinose M. · Okonogi H. · Kawaguchi Y. · Hosoya T. · Nakamura K. · Kataoka E. · Suzuki M. · Koremoto M. · Sumita M. · Shirai T. · Kasai K.
aSecond Department, Central Technology Laboratory, Asahi Kasei Corporation and bLaboratory for Biology, Asahi Kasei Pharma, Fuji; cDivision of Nephrology and Hypertension, Jikei University, School of Medicine, Tokyo; dFuji Daiichi Clinic, Fuji; eDialysis Products Division, Asahi Kasei Medical, Tokyo; fFuji City General Hospital, Fuji, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 1/5/2005
Accepted: 7/22/2005
Published online: 2/15/2006

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 2

ISSN: 0253-5068 (Print)
eISSN: 1421-9735 (Online)

For additional information: http://www.karger.com/BPU

Abstract

Background/Aims: Hemodialysis (HD) therapy may lead to functional changes in patient leukocytes. For example, the upregulation of inflammatory cytokines, such as IL-1β and TNFα, has been well characterized. However, these findings do not explain the entire response of leukocytes in HD. In this study, we carried out a comprehensive gene expression analysis in leukocytes treated with various dialysis membranes using DNA microarrays. The identified gene has the potential to be a new marker for testing dialysis membrane biocompatibility. Methods: Gene expression profiles were compared between a group of leukocytes treated with various dialysis membranes and an untreated group by using DNA microarray analysis. Expression was confirmed by quantitative RT-PCR. The expression of the gene product (leukocyte surface protein) was examined in 20 chronic HD patients by flow cytometry. Results: In addition to the inflammatory cytokines, the urokinase plasminogen activator receptor (uPAR or CD87) gene was induced in leukocytes treated with each dialysis membrane. The extent of induction depended on the membrane’s material composition. The expression of the uPAR (CD87) protein on leukocytes was markedly increased in patients undergoing dialysis therapy. The magnitude of uPAR (CD87) protein expression was correlated with clinical findings, i.e., the degree of leukopenia and the expression of adhesion molecules. Conclusions: The gene and protein expression of uPAR (CD87) depended on the dialysis membrane material and correlated closely with clinical findings. These results suggest that uPAR has the potential to serve as a marker not only for clinical use but also for the development of new dialysis membranes.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 1/5/2005
Accepted: 7/22/2005
Published online: 2/15/2006

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 2

ISSN: 0253-5068 (Print)
eISSN: 1421-9735 (Online)

For additional information: http://www.karger.com/BPU


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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

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