Journal Mobile Options
Table of Contents
Vol. 40, No. 2, 2006
Issue release date: February 2006
Caries Res 2006;40:112–116
(DOI:10.1159/000091056)

The Microbiological Origin of Fluorescence Observed in Plaque on Dentures during QLF Analysis

Coulthwaite L. · Pretty I.A. · Smith P.W. · Higham S.M. · Verran J.
To view the fulltext, log in and/or choose pay-per-view option

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

The aim of this study was to determine the microbiological origin of plaque fluorescence observed during quantitative light-induced fluorescence (QLF) analysis. Plaque was sampled from dentures, because of easy accessibility and the homogeneous background provided by the denture tooth during imaging, and the acknowledged comparability to occlusal plaque. Forty removable poly(methyl methacrylate) dentures were screened for the presence of fluorescent plaque deposits during QLF analysis. Dentures were photographed, QLF images were recorded and samples of fluorescent plaque were taken. Plaque samples were cultured on fastidious anaerobe agar, Wilkins Chalgren agar and Sabourauds dextrose agar. Plates were screened under QLF and fluorescent colonies were subcultured and identified. Areas of red, orange and green fluorescence were detected on the fitting and non-fitting surfaces of dentures. The red and orange fluorescing species were Prevotella melaninogenica, Actinomyces israelii and Candida albicans, which are generally acknowledged to be secondary colonisers, present in more mature plaque. Green fluorescence was observed in streptococcal species (early colonisers) and Fusobacterium nucleatum (important organism in plaque development). Non-fluorescent colonies were also cultured. Plaque which accumulates on susceptible surfaces tends to be associated with caries, but it may be its maturity, rather than the presence of cariogenic streptococci, that is more likely to provide a microbiological link between red fluorescence and caries.



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Bissonnette R, Zeng H, McLean DI, Schreiber WE, Roscoe DL, Lui H: Psoriatic plaques exhibit red autofluorescence that is due to protoporphyrin IX. J Invest Dermatol 1998;111:586–590.
  2. Brazier J: A note on ultra-violet red fluorescence of anaerobic bacteria in vitro. J Appl Bacteriol 1986;60:121–126.
  3. Coulthwaite L, Pretty IA, Smith PW, Higham SM, Verran J: QLF of denture plaque: red fluorescence has microbiological origin. J Dent Res 2005;84(B):93.
  4. Edgerton M, Levine MJ: Characterization of acquired denture pellicle from healthy and stomatitis patients. J Prosthet Dent 1992;68:683–691.
  5. Gibbons RJ, MacDonald JB: Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol 1960;80:164–170.
  6. Kolenbrander PE, Andersen RN, Moore LV: Coaggregation of Fusobacterium nucleatum, Selenomonas flueggei, Selenomonas infelix, Selenomonas noxia, and Selenomonas sputigena with strains from 11 genera of oral bacteria. Infect Immun 1989;57:3194–3203.
  7. König K, Hibst R, Meyer H, Flemming G, Schneckenburger J: Laser-induced autofluorescence of carious regions of human teeth and caries-involved bacteria. SPIE Proc1993;2080:170–180.
  8. Marsh P, Martin MV: Oral Microbiology, ed 4. Oxford, Wright, 1999, pp 61–63.
  9. Neill D: A study of materials and methods employed in cleaning dentures. Br Dent J 1968;124:107–115.
  10. Pratten J, Wilson M, Spratt DA: Characterization of in vitrooral bacterial biofilms by traditional and molecular methods. Oral Microbiol Immunol 2003;18:45–49.
  11. Pretty I, Edgar WM, Higham SM: The use of QLF to quantify in vitro whitening in a product testing model. Aesthetic Dent2001;191:566–569.
  12. Pretty I, Edgar WM, Smith PW, Higham SM: Quantification of dental plaque in the research environment. J Dent 2005;33:193–207.
  13. Theilade E, Budtz-Jorgensen E, Theilade J: Predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa. Arch Oral Biol 1983;28:675–680.
  14. van der Veen M, de Josselin de Jong E: Application of quantitative light-induced fluorescence for assessing early caries lesions; in Faller RV (ed): Assessment of Oral Health. Monographs in Oral Science, vol. 17, Basel, Karger, 2000, pp 144–162.
  15. van der Weijden G, Timmerman MF, Reijerse E, Wolffe GN, van Winkelhoff AJ, van der Velden U: The prevalence of A. actinomycetemcomitans, P. gingivalis and P. intermedia in selected subjects with periodontitis. J Clin Periodontol 1994;21:583–588.
  16. Verran J: Denture plaque, denture stomatitis and the adhesion of Candida albicans to inert materials; in Busscher HE, Evans LV (eds): Oral Biofilms and Plaque Control. Amsterdam, Harwood Academic Publishers, 1999, pp 175–191.
  17. Verran J: Malodour in denture wearers: an ill-defined problem. Oral Dis 2005;11:24–28.


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50