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Vol. 114, No. 1, 2006
Issue release date: May 2006
Cytogenet Genome Res 114:77–82 (2006)
(DOI:10.1159/000091932)

Nuclear DNA content and nuclear and cell volume are positively correlated in angiosperms

Jovtchev G. · Schubert V. · Meister A. · Barow M. · Schubert I.
aInstitute of Plant Genetics and Crop Plant Research (IPK), Gatersleben (Germany); bCentral Laboratory of General Ecology (CLGE), Bulgarian Academy of Science, Sofia (Bulgaria); cAcademic Transfusion Medicine Unit and Cancer Division, Section of Experimental Haematology, Royal Infirmary, Glasgow (UK)

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Abstract

Volumes of flow sorted nuclei were analyzed from two highly endopolyploid (diploids with endopolyploid tissues) species (Arabidopsis thaliana and Barbarea stricta), from a less endopolyploid species (Allium cepa) and from two non-endopolyploid species (Chrysanthemum multicolor and Fritillaria uva-vulpis). Intraspecific as well as interspecific comparisons revealed a highly positive correlation (r >0.99) between DNA content and nuclear volume. No significant differences between expected and measured nuclear volumes were noted indicating that chromatin packing is not increased with increasing DNA content in the tested plant species. In epidermis cells of A. thaliana, A. cepa and Ch. multicolor, a lower (r between 0.6 and 0.7) but significant positive correlation between nuclear volume and cell volume was found. This correlation is compatible with the hypothesis that endopolyploidization (EP = consecutive replication cycles not separated by nuclear and cell divisions) might speed up the growth of endopolyploid species and compensate for small genome size.



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References

  1. Anderson LK, Stack SM, Fox MH, Zhang CS: Relationship between genome size and synaptonemal complex length in higher plants. Exp Cell Res 156:367–378 (1985).
  2. Baluška F, Kubica Š: Relationship between the content of basic nuclear proteins, chromatin structure, rDNA transcription and cell size in different tissues of the maize root apex. J Exp Bot 43:991–996 (1992).
  3. Barow M, Meister A: Endopolyploidy in seed plants is differently correlated to systematics, organ, life strategy and genome size. Plant Cell Environment 26:571–584 (2003).

    External Resources

  4. Beemster GTS, De Vusser K, De Tarvernier E, de Bock K, Inzé D: Variation in growth rate between Arabidopsis ecotypes is correlated with cell division and A-type cyclin-dependent kinase activity. Plant Phys 129:854–864 (2002).
  5. Bennett MD, Leitch IJ: Plant DNA C-values database at: www.rbgkew.org.uk/cval/homepage.html (release 3.0 Dec 2004).
  6. Bennett MD, Leitch IJ: Genome size evolution in plants, in Gregory TR (ed): The Evolution of the Genome, pp 89–162 (Elsevier Academic, Amsterdam 2005).
  7. Bennett MD, Heslop-Harrison JS, Smith JB, Ward JP: DNA density in mitotic and meiotic metaphase chromosomes of plants and animals. J Cell Sci 63:173–179 (1983).
  8. Caceres ME, De Pace C, Mugnozza GTS, Kotsonis P, Ceccarelli M, Cionini PG: Genome size variation within Dasypyrum villosum: correlation with chromosomal traits, environmental factors and plant phenotypic characteristics and behaviour in reproduction. Theor Appl Genet 96:559–567 (1998).
  9. Cavalier-Smith T: Economy, speed and size matter: evolutionary forces driving nuclear genome miniaturization and expansion. Ann Bot 95:147–175 (2005).
  10. Cavalier-Smith T, Beaton MJ: The skeletal function of non-genic nuclear DNA: new evidence from ancient cell chimaeras. Genetica 106:3–13 (1999).
  11. Czeika G: Strukturänderungen endopolyploider Ruhekerne im Zusammenhang mit wechselnder Bündelung der Tochterchromosomen und karyologisch-anatomische Untersuchungen an Sukkulenten. Österr Bot Z 103:536–566 (1956).
  12. Fantes PA, Grant WD, Pritchard RH, Sudbery PE, Wheals AE: The regulation of cell size and the control of mitosis. J Theor Biol 50:213–244 (1976).

    External Resources

  13. Garbary DJ, Clarke B: Intraplant variation in nuclear DNA content in Laminaria saccharina and Alaria esculenta(Phaeophyceae). Bot Mar 45:211–216 (2002).
  14. Gray JD, Koselik P, Høj PB, Coombe BG: Confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue. Plant J 19:229–236 (1999).
  15. Jones RN: B-chromosome systems in flowering plants and animal species. Int Rev Cytol 40:1–100 (1975).
  16. Kudo N, Kimura Y: Nuclear DNA endoreduplication during petal development in cabbage: relationship between ploidy levels and cell size. J Exp Bot 53:1017–1023 (2002).
  17. Lee H, Chiou D, Chen W, Markhart AH, Chen Y, Lin T: Dynamics of cell growth and endoreduplication during orchid flower development. Plant Sci 166:659–667 (2004).
  18. Meagher TR, Costich DE: Nuclear DNA content and floral evolution in Silene latifolia. Proc Roy Soc Biol Sci Series B 263:1455–1460 (1996).

    External Resources

  19. Melaragno JE, Mehrotra B, Coleman AW: Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis. Plant Cell 5:1661–1668 (1993).
  20. Murray BG: When does intraspecific C-value variation become taxonomically significant? Ann Bot 95:119–125 (2005).
  21. Price HJ, Sparrow AH, Nauman AF: Correlation between nuclear volume, cell volume and DNA content in meristematic cells of herbaceous angiosperms. Experimentia 29:1028–1029 (1973).
  22. Prokopowich CD, Gregory TR, Crease TJ: The correlation between rDNA copy number and genome size in eukaryotes. Genome 46:48–50 (2003).
  23. Raina SN, Bisht MS: DNA amounts and chromatin compactness in Vicia. Genetica 77:65–77 (1988).
  24. Sugimoto-Shirasu K, Roberts K: ‘Big it up’: endoreduplication and cell-size control in plants. Curr Opin Plant Biol 6:544–553 (2003).
  25. Tschermak-Woess E, Hasitschka G: İber die endomitotische Polyploidisierung im Zuge der Differenzierung von Trichomen und Trichozyten bei Angiospermen. Österr Bot Z 101:79–117 (1954).
  26. Uozo S, Ikehashi H, Omido N, Ohtsubo H, Ohtsubo E, Fukui K: Repetitive sequences: causes for variation in genome size and chromosome morphology in the genus Oryza. Plant Mol Biol 35:791–799 (1997).
  27. Vinogradov AE: Genome size and chromatin condensation in vertebrates. Chromosoma 113:362–369 (2005).
  28. White J, Rees H: Chromosome weights and measures in Petunia. Heredity 58:139–143 (1987).


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