Journal Mobile Options
Table of Contents
Vol. 115, No. 1, 2006
Issue release date: September 2006

Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues

Kiuchi S. · Yamada T. · Kiyokawa N. · Saito T. · Fujimoto J. · Yasue H.
To view the fulltext, log in and/or choose pay-per-view option

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

Taste receptor family 1 member 3, TAS1R3, is shown to be involved in sweet and umami tastes in mouse, and the nucleotide sequence of the gene has been reported in rat, gorilla, and human. Pigs are frequently used as models for human diseases, and are also considered to be source animals for xenotransplantation to humans due to their anatomical and physiological similarities to humans. Therefore, in the present study, the genomic structure of the swine TAS1R3 gene was determined, and TAS1R3 expression was studied in various swine tissues. The gene was shown to reside on swine chromosome 6q22→q23, from which three types of mRNAs were generated: 3,752 bp derived from six exons in tongue, 3,704 bp from six exons and 3,630 bp from seven exons in testis. The 6 exons/5 introns were structurally similar to those of humans and mice, but the 7 exons/6 introns structure of TAS1R3 was first observed in swine. High expressions of TAS1R3 were revealed in tongue, kidney, and testis by real-time PCR. The expression profile of the tissues except for kidney was similar to that of mouse. When in situ hybridization using an RNA probe for TAS1R3 was performed on swine tongue and testis tissues, TAS1R3 expressions were revealed in tongue circumvallate papillae, fungiform papillae, mucosal epithelium, follicular B lymphocytes, lymphocytes in submucosal tissues of lingual tonsil, and spermatogenic cells. Using peripheral mature B lymphocytes, the expression of TAS1R3 in B lymphocytes was further confirmed by real-time PCR and sequencing of the real-time PCR product.



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Adler E, Hoon MA, Mueller KL, Chandrashekar J, Ryba NJ, Zuker CS: A novel family of mammalian taste receptors. Cell 100:693–702 (2000).
  2. Awata T, Yamakuchi H, Kumagai M, Yasue H: Assignment of the tenascin gene (HXB) to swine chromosome 1q21.1→q21.3 by fluorescence in situ hybridization. Cytogenet Cell Genet 69:33–34 (1995).
  3. Bernhardt SJ, Naim M, Zehavi U, Lindemann B: Changes in IP3 and cytosolic Ca2+ in response to sugars and non-sugar sweeteners in transduction of sweet taste in the rat. J Physiol 490:325–336 (1996).
  4. Bornstein P, McKay J, Morishima JK, Devarayalu S, Gelinas RE: Regulatory elements in the first intron contribute to transcriptional control of the human alpha 1(I) collagen gene. Proc Natl Acad Sci USA 84:8869–8873 (1987).
  5. Cummings TA, Daniels C, Kinnamon SC: Sweet taste transduction in hamster: sweeteners and cyclic nucleotides depolarize taste cells by reducing a K+ current. J Neurophysiol 75:1256–1263 (1996).
  6. Gilbertson TA, Avenet P, Kinnamon SC, Roper SD: Proton currents through amiloride-sensitive Na channels in hamster taste cells. Role in acid transduction. J Gen Physiol 100:803–824 (1992).
  7. Gilbertson TA, Damak S, Margolskee RF: The molecular physiology of taste transduction. Curr Opin Neurobiol 10:519–527 (2000).
  8. Kitagawa M, Kusakabe Y, Miura H, Ninomiya Y, Hino A: Molecular genetic identification of a candidate receptor gene for sweet taste. Biochem Biophys Res Commun 283:236–242 (2001).
  9. Kiuchi S, Inage Y, Hiraiwa H, Uenishi H, Yasue H: Assignment of 280 swine genomic inserts including 31 microsatellites from BAC clones to the swine RH map (IMpRH map). Mamm Genome 13:80–88 (2002).
  10. Kretz O, Barbry P, Bock R, Lindemann B: Differential expression of RNA and protein of the three pore-forming subunits of the amiloride-sensitive epithelial sodium channel in taste buds of the rat. J Histochem Cytochem 47:51–64 (1999).
  11. Li X, Staszewski L, Xu H, Durick K, Zoller M, Adler E: Human receptors for sweet and umami taste. Proc Natl Acad Sci USA 99:4692–4696 (2002).
  12. Lin W, Burks CA, Hansen DR, Kinnamon SC, Gilbertson TA: Taste receptor cells express pH-sensitive leak K+ channels. J Neurophysiol 92:2909–2919 (2004).
  13. Lindemann B: Receptors and transduction in taste. Nature 413:219–225 (2001).
  14. Max M, Shanker YG, Huang L, Rong M, Liu Z, Campagne F, Weinstein H, Damak S, Margolskee RF: Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac. Nat Genet 28:58–63 (2001).
  15. Mikawa S, Akita T, Hisamatsu N, Inage Y, Ito Y, Kobayashi E, Kusumoto H, Matsumoto T, et al: A linkage map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs. Anim Genet 30:407–417 (1999).
  16. Milan D, Hawken R, Cabau C, Leroux S, Genet C, Lahbib Y, Tosser G, Robic A, et al: IMpRH server: an RH mapping server available on the Web. Bioinformatics 16:558–559 (2000).
  17. Nelson G, Hoon MA, Chandrashekar J, Zhang Y, Ryba NJ, Zuker CS: Mammalian sweet taste receptors. Cell 106:381–390 (2001).
  18. Nelson G, Chandrashekar J, Hoon MA, Feng L, Zhao G, Ryba NJ, Zuker CS: An amino-acid taste receptor. Nature 416:199–202 (2002).
  19. Ohtsuki T, Furuya S, Yamada T, Nomura S, Hata J, Yabe Y, Hosoda Y: Gene expression of noncollagenous bone matrix proteins in the limb joints and intervertebral disks of the twy mouse. Calcif Tissue Int 63:67–172 (1998).
  20. Reed DR, Li S, Li X, Huang L, Tordoff MG, Starling-Roney R, Taniguchi K, West DB, et al: Polymorphisms in the taste receptor gene (Tas1r3) region are associated with saccharin preference in 30 mouse strains. J Neurosci 24:938–946 (2004).
  21. Schultz JR, Tansey T, Gremke L, Storti RV: A muscle-specific intron enhancer required for rescue of indirect flight muscle and jump muscle function regulates Drosophila tropomyosin I gene expression. Mol Cell Biol 11:1901–1911 (1991).
  22. Suzuki K, Asakawa S, Iida M, Shimanuki S, Fujishima N, Hiraiwa H, Murakami Y, Shimizu N, Yasue H: Construction and evaluation of a porcine bacterial artificial chromosome library. Anim Genet 31:8–12 (2000).
  23. Suzuki Y, Sugano S: Construction of a full-length enriched and a 5′-end enriched cDNA library using the oligo-capping method. Methods Mol Biol 221:73–91 (2003).
  24. Tumbelson ME, Schook LB: Advances in swine in biomedical research, in Tumbelson ME, Schook LB (eds):Advances in Swine in Biomedical Research, pp 1–4 (Plenum Press, New York 1996).
  25. Yasue H, Ishibashi M: The oncogenicity of avian adenoviruses. III. In situ DNA hybridization of tumor line cells localized a large number of a virocellular sequence in few chromosomes. Virology 116:99–115 (1982).
  26. Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, et al: Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82:182–188 (1998).
  27. Zhao GQ, Zhang Y, Hoon MA, Chandrashekar J, Erlenbach I, Ryba NJ, Zuker CS: The receptors for mammalian sweet and umami taste. Cell 115:255–266 (2003).


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50