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Table of Contents
Vol. 183, No. 2, 2006
Issue release date: October 2006
Section title: Original Paper
Cells Tissues Organs 2006;183:68–79
(DOI:10.1159/000095511)

Influence of Platelet-Rich Plasma on Osteogenic Differentiation of Mesenchymal Stem Cells and Ectopic Bone Formation in Calcium Phosphate Ceramics

Kasten P. · Vogel J. · Luginbühl R. · Niemeyer P. · Weiss S. · Schneider S. · Kramer M. · Leo A. · Richter W.
aDivision of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg and bInstitute for Clinical Transfusion Medicine and Cellular Therapy Heidelberg, Heidelberg, cDepartment for Orthopaedics and Traumatology, University of Freiburg, Freiburg, and dClinic for Small Animals, Surgery, Justus Liebig University Giessen, Giessen, Germany; eDr. h.c. Robert Mathys Foundation, Bettlach, Switzerland

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/14/2006
Published online: 10/20/2006

Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 3

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Platelet-rich plasma (PRP) contains a mixture of growth factors that play an important role in wound and fracture healing. While PRP enhanced bone formation by autogenous cancellous bone grafts, its influence in combination with different bone substitutes remained unknown. This study evaluated the effect of PRP on osteogenic differentiation and ectopic bone formation of human mesenchymal stem cells (MSC) in distinct resorbable calcium phosphate ceramics. Calcium-deficient hydroxyapatite (CDHA) blocks with a large specific surface area (48 m2/g) and β-tricalcium phosphate (β-TCP) with a low specific surface area (<0.5 m2/g) were loaded with 2 × 105 bone marrow-derived MSC. Half of the specimens were treated with 5-fold concentrated PRP. Biocomposites were implanted subcutaneously into SCID mice or kept under osteogenic culture conditions for 2 weeks before implantation. The addition of PRP increased the specific alkaline phosphatase (ALP) activity (p = 0.012) in undifferentiated MSC/CDHA composites but not in MSC/β-TCP composites. Osteogenic preinduction was ineffective for CDHA and reduced ALP activity of β-TCP composites significantly at explantation. Ectopic bone formation was stronger in MSC/CDHA (7/32) compared to MSC/β-TCP (2/30) composites, but no influence of PRP was evident. In conclusion, the effect of PRP depended on the type of ceramic and the differentiation status of the MSC, and enhanced ALP activity of MSC on the high surface scaffold CDHA only, but PRP did not improve osteogenesis in our setting.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/14/2006
Published online: 10/20/2006

Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 3

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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