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Vol. 142, No. 1, 2007
Issue release date: December 2006
Section title: Original Paper
Int Arch Allergy Immunol 2007;142:40–50
(DOI:10.1159/000095997)

Factors Affecting Human IgE and IgG Responses to Allergen-Like Schistosoma mansoni Antigens: Molecular Structure and Patterns of in vivo Exposure

Fitzsimmons C.M. · McBeath R. · Joseph S. · Jones F.M. · Walter K. · Hoffmann K.F. · Kariuki H.C. · Mwatha J.K. · Kimani G. · Kabatereine N.B. · Vennervald B.J. · Ouma J.H. · Dunne D.W.
aDepartment of Pathology, University of Cambridge, and bMRC Biostatistics Unit, Institute of Public Health, Cambridge, UK; cInstitute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa; dDivision of Vector-Borne Diseases, Kenyan Ministry of Health, and eKenya Medical Research Institute, Nairobi, Kenya; fVector Control Division, Ugandan Ministry of Health, Kampala, Uganda; gDBL-Institute for Health, Research and Development, Charlottenlund, Denmark

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/15/2006
Accepted: 7/27/2006
Published online: 10/2/2006

Number of Print Pages: 11
Number of Figures: 5
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: The human IgE response is associated with allergy and with host defence against parasitic worms. A response to Sm22.6, the dominant IgE antigen in adult Schistosoma mansoni worms, correlates with resistance to re-infection after treatment. Sm22.6 is one of a family of EF-hand containing parasite proteins with sequence similarity to dynein light chain (DLC) and with major non-parasite allergens. Here we compare human IgE and IgG responses to other family members, Sm20.8 and Sm21.7, as well as to SmDLC1, relating these to antigen structure and expression in parasite life stages. Methods: Recombinant antigens were used in ELISA to measure antibody isotype responses in 177 cases from an endemic area, before and 7 weeks after treatment. Parasite antigen expression was assessed by RT-PCR and Western blotting. Results: Levels of antibodies to Sm22.6 and Sm20.8 (but not to Sm21.7 or SmDLC1) showed posttreatment increases in all but young children. Many produced IgE to Sm22.6 and Sm20.8 (2 EF-hands), few to Sm21.7 (1 EF-hand) or SmDLC1 (no EF-hands). Sm21.7 was expressed in cercariae, adults and eggs, Sm22.6 and Sm20.8 were concentrated in the adult. Conclusions: These studies suggest that IgE antigens Sm22.6 and Sm20.8 are only released to boost antibodies when adult worms die, whilst Sm21.7 and SmDLC1 are released constantly from eggs dying in host tissue. IgE responses to these allergen-like molecules may be influenced by patterns of exposure and the number of EF-hand motifs.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/15/2006
Accepted: 7/27/2006
Published online: 10/2/2006

Number of Print Pages: 11
Number of Figures: 5
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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