For nearly a century, lactobacilli (LB) in the oral cavity have been generally associated with dental caries. Here, we characterized the LB isolated from the saliva of 6 women with active caries using genetic-based taxonomical identification methods. From each subject, 30 isolates growing on Rogosa medium and presumed to be LB were analyzed. Of the 180 isolates, 176 were further characterized by biotyping, DNA melting points, DNA chromosomal fingerprinting, genotyping, and phylogenetic cluster assessment. We found a total of 30 unique genotypes of LB in the saliva of caries-active women, with each woman harboring between 2 and 8 distinct genotypes. Although Lactobacillus vaginalis, L. fermentum, and L. salivarius were found in 4 of 6 of the subjects, results from other studies using comparable methods show an entirely different array of LB associated with caries. These collective observations lead us to surmise that LB associated with dental caries are likely exogenous and opportunistic colonizers, arising from food or other reservoirs outside the oral cavity.
© 2007 S. Karger AG, Basel
- Dental caries
- Phylogeny, bacterial diversity
- 16S rDNA
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P.W. Caufield, DDS, PhD
New York University, College of Dentistry
345 E. 24th Street, Room 1024, Kriser Building
New York, NY 10010 (USA)
Tel. +1 212 998 9603, Fax +1 212 995 3994, E-Mail email@example.com
Received: December 15, 2005
Accepted after revision: March 23, 2006
Number of Print Pages : 7
Number of Figures : 2, Number of Tables : 0, Number of References : 42
Vol. 41, No. 1, Year 2007 (Cover Date: December 2006)
Journal Editor: Shellis, R.P.(Bristol)
ISSN: 0008–6568 (print), 1421–976X (Online)
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