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Vol. 20, No. 2, 2007
Issue release date: March 2007
Skin Pharmacol Appl Skin Physiol 2007;20:77–84
(DOI:10.1159/000097654)

Influence of Different Anesthetics on Skin Oxygenation Studied by Electron Paramagnetic Resonance in vivo

Abramovic Z. · Sentjurc M. · Kristl J. · Khan N. · Hou H. · Swartz H.M.
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Abstract

The effects of two general anesthetics on skin oxygenation in mice are evaluated by electron paramagnetic resonance oximetry. Up to now no data on the effects of different anesthetics on skin oxygenation could be found. In this study animals were anesthetized with ketamine/xylazine or isoflurane, and partial pressure of oxygen (pO2) in the skin, heart rate and hemoglobin oxygen saturation were followed as a function of time and inhaled oxygen concentration. The skin pO2 significantly increased continuously for about 60 min in mice anesthetized with isoflurane and remained constant after that. During ketamine/xylazine anesthesia, the pO2 in the skin only slightly decreased. The skin pO2 increased with higher inspired oxygen concentrations for both anesthetics groups. When breathing 21% oxygen, mice anesthetized with isoflurane had two-fold higher pO2 in the skin compared to mice anesthetized with ketamine/xylazine. The heart rate was significantly lower in animals anesthetized with ketamine/xylazine, while hemoglobin saturation was almost the same in both groups at all inhaled oxygen concentrations. These results show that the type of anesthesia is an important parameter that needs to be considered in experiments where skin pO2 is followed.



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References

  1. Artmann C, Röding J, Stanzl K, Zastrow L: Oxygen in the skin – a new parameter of skin ageing. Söfw J 1993;15:6–8.
  2. Ratnaraj J, Kabon B, Talcott MR, Sessler DI, Kurz A: Supplemental oxygen and carbon dioxide each increase subcutaneous and intestinal intramural oxygenation. Anesth Analg 2004;99:207–211.
  3. Gallez B, Baudelet C, Jordan BF: Assessment of tumor oxygenation by electron paramagnetic resonance: principles and applications. NMR Biomed 2004;17:240–262.
  4. Tandara AA, Mustoe TA: Oxygen in wound healing – more than a nutrient. World J Surg 2004;28:294–300.
  5. Greif R, Akca O, Horn EP, Kurz A, Sessler DI: Supplemental perioperative oxygen to reduce the incidence of surgical-wound infection. N Engl J Med 2000;342:161–167.
  6. Kristl J, Abramovic Z, Sentjurc M: Skin oxygenation after topical application of liposome-entrapped benzyl nicotinate as measured by EPR oximetry in vivo: influence of composition and size. AAPS Pharm Sci 2003;5:E2.

    External Resources

  7. Fuchs J, Thiele J: The role of oxygen in cutaneous photodynamic therapy. Free Radic Biol Med 1998;24:835–847.
  8. Swartz HM: Measuring real levels of oxygen in vivo: opportunities and challenges. Biochem Soc Trans 2002;30:248–252.
  9. Dunn JF, Swartz HM: In vivo electron paramagnetic resonance oximetry with particulate materials. Methods 2003;30:159–166.
  10. Swartz HM, Khan N, Buckey J, Comi R, Gould L, Grinberg O, Hartford A, Hopf H, Hou H, Hug E, Iwasaki A, Lesniewski P, Salikhov I, Walczak T: Clinical applications of EPR: overview and perspectives. NMR Biomed 2004;17:335–351.
  11. Krzic M, Sentjurc M, Kristl J: Improved skin oxygenation after benzyl nicotinate application in different carriers as measured by EPR oximetry in vivo. J Control Release 2001;70:203–211.
  12. Gould LJ, Mushkudiani J, Hou H, Grinberg OY, Grinberg S, Swartz HM: Use of EPR spectroscopy to measure tissue oxygen in an ischemic flap model. Wound Repair Regen 2004;12:A29.

    External Resources

  13. Sessler DI, McGuire J, Moayeri A, Hynson J: Isoflurane-induced vasodilation minimally increases cutaneous heat loss. Anesthesiology 1991;74:226–232.
  14. Greenblatt EP, Loeb AL, Longnecker DE: Endothelium-dependent circulatory control – a mechanism for the differing peripheral vascular effects of isoflurane versus halothane. Anesthesiology 1992;77:1178–1185.
  15. Honzak L, Sentjurc M, Swartz HM: In vivo EPR of topical delivery of a hydrophilic substance encapsulated in multilamellar liposomes applied to the skin of hairless and normal mice. J Control Release 2000;66:221–228.
  16. Thurman JC, Tranquilli WJ, Benson GJ: Preanesthetic and anesthetic adjuncts; in Thurman JC, Tranquilli WJ, Benson GJ (eds): Lumb and Jones’ Veterinary Anesthesia. Baltimore, Williams & Wilkins, 1996, pp 183–209.
  17. Carpenter JW: Exotic Animal Formulary, ed. 3- St Louis, Elsevier Saunders, 2005.
  18. Sentjurc M, Kristl J, Abramovic Z: Transport of liposome-entrapped substances into skin as measured by electron paramagnetic resonance oximetry in vivo. Methods Enzymol 2004;387:267–287.
  19. Walczak T, Lesniewski P, Salikhov I, Sucheta A, Szybinski K, Swartz HM: L-Band EPR spectrometer for the use in vivo and in the studies of aqueous biological samples. Rev Sci Instrum 2005;76:1–6
  20. Wang W, Vadgama P: O2 microsensors for minimally invasive tissue monitoring. J R Soc Interface 2004;1:109–117.
  21. Stucker M, Struk A, Altmeyer P, Herde M, Baumgartl H, Lubbers DW: The cutaneous uptake of atmospheric oxygen contributes significantly to the oxygen supply of human dermis and epidermis. J Physiol 2002;538.3:985–994.
  22. Evans SM, Schrlau AE, Chalian AA, Zhang P, Koch CJ: Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. J Invest Dermatol 2006, in press.
  23. Zuurbier CJ, Emons VM, Ince C: Hemodynamics of anesthetized ventilated mouse models: aspects of anesthetics, fluid support, and strain. Am J Physiol Heart Circ Physiol 2002;282:H2099–H2105.
  24. Uechi M, Asai K, Osaka M, Smith A, Sato N, Wagner TE, Ishikawa Y, Hayakawa H, Vatner DE, Shannon RP, Homcy CJ, Vatner SF: Depressed heart rate variability and arterial baroreflex in conscious transgenic mice with overexpression of cardiac Gsalpha. Circ Res 1998;82:416–423.
  25. Butz GM, Davisson RL: Long-term telemetric measurement of cardiovascular parameters in awake mice: a physiological genomics tool. Physiol Genomics 2001;5:89–97.
  26. Hou H, Grinberg OY, Taie S, Leichtweis S, Miyake M, Grinberg S, Xie H, Csete M, Swartz HM: Electron paramagnetic resonance assessment of brain tissue oxygen tension in anesthetized rats. Anesth Analg 2003;96:1467–1472.
  27. Lei H, Grinberg O, Nwaigwe CI, Hou HG, Williams H, Swartz HM, Dunn JF: The effects of ketamine–xylazine anesthesia on cerebral blood flow and oxygenation observed using nuclear magnetic resonance perfusion imaging and electron paramagnetic resonance oximetry. Brain Res 2001;913:174–179.
  28. Roth DM, Swaney JS, Dalton ND, Gilpin EA, Ross J Jr: Impact of anesthesia on cardiac function during echocardiography in mice. Am J Physiol Heart Circ Physiol 2002;282:H2134–H2140.
  29. Baudelet C, Gallez B: Effect of anesthesia on the signal intensity in tumors using BOLD-MRI: comparison with flow measurements by Laser Doppler flowmetry and oxygen measurements by luminescence-based probes. Magn Reson Imaging 2004;22:905–912.
  30. Liu KJ, Bacic G, Hoopes PJ, Jiang J, Du H, Ou LC, Dunn JF, Swartz HM: Assessment of cerebral pO2 by EPR oximetry in rodents: effects of anesthesia, ischemia, and breathing gas. Brain Res 1995;685:91–98.
  31. Chaves AA, Weinstein DM, Bauer JA: Non-invasive echocardiographic studies in mice: influence of anesthetic regimen. Life Sci 2001;69:213–222.


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