Original Paper

A Culture Device Demonstrates that Hydrostatic Pressure Increases mRNA of RGS5 in Neuroblastoma and CHC1-L in Lymphocytic Cells
Yoshinobu Manome^a,b, Natsumi Saeki^a, Hisao Yoshinaga^a, Michiko Watanabe^a, Shuichi Mizuno^c

Departments of
^aMicrobiology and
^bNeurosurgery, Jikei University School of Medicine, Tokyo, Japan;
^cDepartment of Orthopedic Surgery, Brigham and Women's Hospital, Boston, Mass., USA

Address of Corresponding Author

Cells Tissues Organs 2003;174:155-161 (DOI: 10.1159/000072718)

  Key Words

• Cell culture system
• Hydrostatic pressure
• Neuroblastoma
• RGS5
• Lymphocyte
• CHC1-L

  Abstract

Previous studies demonstrated that mechanical forces affect a wide range of cellular behaviors. These forces regulate important cellular responses in the human body and consist of gravity, hydrostatic pressure, stretch, and shear stress, which is exerted on the vascular system by the passage of blood flow. We reasoned that these forces might be significant and dynamic regulators of cellular functions within the human body. While cellular effects of stretch and shear stress have been studied particularly with endothelial cells, little is known about the effects of gravity and hydrostatic pressure to cells. To examine the direct effect of hydrostatic pressure, we developed a culture device to confer hydrostatic pressures to cells ranging from 0 to 1,000 psi. We subjected human neuroblastoma cells and rIL-2-activated lymphocytes to a constant pressure of 20 or 100 psi for 48 h and attempted to identify genes regulated by hydrostatic pressure. Genes of regulator of G-protein signaling 5 in neuroblastoma cells and CHC1-L in lymphocytes increased after exposure to hydrostatic pressure. The results demonstrated that hydrostatic pressure directly regulates the expression of specific genes in mammalian cells. Moreover, there may be some underlying mechanisms that have common effects in altered physical environments. Our in vitro culture system may provide some insight into the mechanisms through the intracellular processes affected by mechanical forces.

Copyright © 2003 S. Karger AG, Basel

  Author Contacts

Yoshinobu Manome, MD, PhD
3-25-8 Nishi-shinbashi, Minato-Ku
Tokyo, 105-8461 (Japan)
Tel. +81 3 3433 1111 (ext. 2246), Fax +81 44 955 9437, E-Mail manome@jikei.ac.jp

  Article Information

Accepted after revision: May 23, 2003
Number of Print Pages : 7
Number of Figures : 2, Number of Tables : 1, Number of References : 44