Chemotherapy
Microbiology
Possible Role of sarA in Dehydroepiandrosterone-Mediated Increase in Staphylococcus aureus Resistance to VancomycinPlotkin B.J.a · Konaklieva M.baDepartment of Microbiology and Immunology, Midwestern University, Downers Grove, Ill., bDepartment of Chemistry, American University, Washington D.C., USA
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Article / Publication Details
Received: November 29, 2005
Accepted: January 31, 2006
Published online: March 14, 2007
Issue release date: April 2007
Number of Print Pages: 4
Number of Figures: 2
Number of Tables: 0
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
Abstract
Background: Dehydroepiandrosterone (DHEA), a steroid present throughout life, can induce an increase in resistance to vancomycin in methicillin-sensitive and methicillin-resistant clinical isolates of Staphylococcus aureus. Methods: The in vitro effect of DHEA on vancomycin killing of S. aureus with mutations in sarA and/or agr was determined by standard microtiter protocols and time to kill determinations. Results: Of the isolates tested, the strain with a deletion in sarAderived from a DHEA- responsive parent was not protected from vancomycin killing by DHEA. However, DHEA significantly (p < 0.01) slowed the rate of vancomycin killing of sarA–. Conclusion: These data indicate that sarA may play a role in DHEA-mediated protection from vancomycin killing of S. aureus.
© 2007 S. Karger AG, Basel
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References
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Article / Publication Details
Received: November 29, 2005
Accepted: January 31, 2006
Published online: March 14, 2007
Issue release date: April 2007
Number of Print Pages: 4
Number of Figures: 2
Number of Tables: 0
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
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