International Archives of Allergy and Immunology
Original Paper
Involvement of Galectin-9 in Guinea Pig Allergic Airway InflammationYamamoto H.a, b · Kashio Y.a · Shoji H.c · Shinonaga R.d · Yoshimura T.e · Nishi N.c, d · Nabe T.b · Nakamura T.c · Kohno S.b · Hirashima M.a, daDepartment of Immunology and Immunopathology, Kagawa University Faculty of Medicine, Kagawa, bDepartment of Pharmacology, Kyoto Pharmaceutical University, Kyoto, cDepartment of Endocrinology, Kagawa University Faculty of Medicine, and dGalpharma Co., Ltd., Kagawa, Japan; eLaboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Md., USA
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Int Arch Allergy Immunol 2007;143:95–105
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Article / Publication Details
First-Page Preview
Published online: May 31, 2007
Issue release date: June 2007
Number of Print Pages: 11
Number of Figures: 6
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
Abstract
Background: There is little information about the involvement of galectin-9 (Gal-9) in allergic inflammation. Thus, we investigated the role of Gal-9 in asthma model guinea pigs. Methods: Airway resistance (Raw) was measured using a double-flow plethysmograph system. Gal-9 expression in the lung was assessed by Western blot and immunohistochemistry. Eosinophil chemotactic activity was evaluated in a chamber containing a polyvinylpyrolidone-free membrane. Cell apoptosis was analyzed on a flowcytometry with propidium iodide. Results: In cloning guinea pig Gal-9 we identified three isoforms that differ only in the length of their linker peptides, just as with human Gal-9. Guinea pig Gal-9 was found to be a chemoattractant for eosinophils and to promote induction of apoptosis in sensitized but not non-sensitized T lymphocytes. In allergic airway hypersensitivity model, a low level of Gal-9 expression was observed in the nonsensitized/nonchallenged group, but upregulation was detected at 7 h after challenge and sustained up to 24 h. Such upregulation correlated with elevation of eosinophil peroxidase activity but not with increased Raw. Conclusions: The present results provide evidence that Gal-9 is not involved in airway hypersensitivity, but is partly involved in prolonged eosinophil accumulation in the lung.
© 2007 S. Karger AG, Basel
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Article / Publication Details
First-Page Preview
Published online: May 31, 2007
Issue release date: June 2007
Number of Print Pages: 11
Number of Figures: 6
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
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