Molecular Characterization of Arginine Kinase, an Allergen from the Shrimp Litopenaeus vannameiGarcía-Orozco K.D. · Aispuro-Hernández E. · Yepiz-Plascencia G. · Calderón-de-la-Barca A.M. · Sotelo-Mundo R.R.
Centro de Investigación en Alimentación y Desarrollo, Hermosillo, México
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Background: Consumption of seafood can produce allergic symptoms in susceptible individuals and crustacean allergies are the most frequently reported causes of allergic reactions. Methods: An allergen from the muscle of the white shrimp Litopenaeus vannamei was purified by ion exchange chromatography and identified by mass spectrometry of tryptic peptides and its specific enzymatic activity. Moreover, the corresponding full-length cDNA was obtained from an L. vannamei muscle cDNA library. Results: A 40-kDa protein was purified and identified as arginine kinase and its cDNA of 1.4 kb encoded a 356 amino acid protein. The obtained arginine kinase was recognized by IgE in serum from shrimp-allergic individuals using ELISA and immunoblotting analysis. Conclusions: This is the first allergen reported for the Pacific white shrimp species; it was named Lit v 2 and has a 96% identity to Pen m 2 from Penaeus monodon.
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- Binder M, Mahler V, Hayek B, Sperr W, Scholler M, Prozell S, Weidermann G, Valent P, Valenta R, Duchene M: Molecular and immunological characterization of arginine kinase from the Indianmeal Moth, Plodia interpunctella, a novel cross-reactive invertebrate pan-allergen. J Immunol 2001;167:5470–5477.
- Yu CJ, Lin YF, Chiang BL, Chow LP: Proteomics and immunological analysis of a novel shrimp allergen, Pen m 2. J Immunol 2003;170:445–453.
- Kalogeromitros D, Makris M, Gregoriou S, Chliva C, Katoulis A, Papaioannou D, Rigopoulos D: IgE-mediated sensitization in seafood processing workers. Allergy Asthma Proc 2006;27:399–403.
- Daul CB, Slattery M, Reese G, Lehrer SB: Identification of the major brown shrimp (Penaeus aztecus) allergen as the muscle protein tropomyosin. Int Arch Allergy Immunol 1994;105:49–55.
O’Neil CE, Lehrer SB. Seafood allergy and allergens – a review. Food Tech 1995;49:103–116.
Leung PSC, Chu KH: Molecular and Immunological characterization of shellfish allergens. Front Biosci 1998;3:306–312.
- Hoffman DR, Day ED Jr, Miller JS: The major heat stable allergen of shrimp. Ann Allergy 1981;47:17–22.
- Nagpal S, Rajappa L, Metcalfe DD, Subba-Rao PV: Isolation and characterization of heat-stable allergens from shrimp (Penaeus indicus). J Allergy Clin Immunol 1989;83:26–36.
- Asturias JA, Gómez-Bayón N, Arilla MC, Martínez A, Palacios R, Sánchez-Gascón F, Martínez J: Molecular characterization of American cockroach tropomyosin (Periplaneta americana allergen 7), a cross-reactive allergen. J Immunol 1999;162:4342–4348.
Leung PSC, Chen-Chen Y, Mykles DL, Kuen-Chow W, Pang-Li C, Hou-Chu K: Molecular identification of the lobster muscle protein tropomyosin as a seafood allergen. Mol Mar Biol Biotech 1998;7:12–20.
- Yao C-L, Wu C-G, Xiang J-H, Dong B: Molecular cloning and response to laminarin stimulation of arginine kinase in haemolymph in Chinese shrimp, Fenneropenaeus chinensis. Fish Shell Immunol 2005;9:317–329.
- Uda K, Fujimoto N, Akiyama Y, Mizuta K, Tanaka K, Ellington WR, Suzuki T: Evolution of the arginine kinase gene family. Comp Biochem Physiol Part D Genomics Proteomics 2006;1:209–218.
- Sookrung N, Chaicumpa W, Tungtrongchitr A, Vichyanond P, Bunnag C, Ramasoota P, Tongtawe P, Sakolvaree Y, Tapchaisri P: Periplaneta americana arginine kinase as a major cockroach allergen among Thai patients with major cockroach allergies. Environ Health Perspect 2006;114:875–880.
Reddy SR, Roustan C, Benyamin Y: Purification and properties of two molecular forms of arginine kinase from the adductor muscle of the scallop, Pecten maximus. Comp Biochem PhysiolB 1991;99:387–394.
FAO Inland Water Resources and Aquaculture Service: Review of the state of world aquaculture. FAO Fisheries Circular 2003;886.
- Cheng ZJ, Hardy RW: Protein and lipid sources affect cholesterol concentrations of juvenile Pacific white shrimp, Litopenaeus vannamei (Boone). J Anim Sci 2004;82:1136–1145.
- Szasz G, Gruber W, Bernt E: Creatine kinase in serum. 1. Determination of optimum reaction conditions. Clin Chem 1976;22:650–656.
- Donham RT, Morin D, Jewell WT, Lame MW, Segall HJ, Tjeerdema RS: Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquat Toxicol 2005;73:221–229.
- Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970;227:680–685.
- Blethen S: Arginine kinase (arthropod muscle). Methods Enzymol 1970;17:330–335.
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 1990;215:403–410.
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680.
- Craig R, Cortens JP, Beavis RC: The use of proteotypic peptide libraries for protein identification. Rapid Commun Mass Spectrom 2005;19:1844–1850.
- Reese G, Ayuso R, Lehrer SB: Tropomyosin: an invertebrate pan-allergen. Int Arch Allergy Immunol 1999;119:247–258.
- Gutierrez-Millan LE, Peregrino-Uriarte AB, Sotelo-Mundo R, Vargas-Albores F, Yepiz-Plascencia G: Sequence and conservation of a rRNA and tRNAVal mitochondrial gene fragment from Penaeus californiensis and comparison with Penaeus vannamei and Penaeus stylirostris. Mar Biotechnol (NY) 2002;4:392–398.
- Aalberse RC: Food allergens. Environ Toxicol Pharmacol 1997;4:55–60.
- Besler M, Steinhart H, Paschke A: Stability of food allergens and allergenicity of processed foods. J Chromatogr B Biomed Sci Appl 2001;756:207–228.
- Bredehorst R, David K: What establishes a protein as an allergen? J Chromatogr B Biomed Sci Appl 2001;756:33–40.
- Kimber I, Kerkvliet NI, Taylor SL, Astwood JD, Sarlo K, Dearman RJ: Toxicology of protein allergenicity: prediction and characterization. Toxicol Sci 1999;48:157–162.
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