Gerontology
Experimental Section
‘In vitro’ Protective Effect of a Hydrophilic Vitamin E Analogue on the Decrease in Levels of Elongation Factor 2 in Conditions of Oxidative StressArgüelles S. · Machado A. · Ayala A.Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España
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Gerontology 2007;53:282–288
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Article / Publication Details
First-Page Preview
Received: January 17, 2007
Accepted: February 20, 2007
Published online: May 16, 2007
Issue release date: August 2007
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 0304-324X (Print)
eISSN: 1423-0003 (Online)
For additional information: https://www.karger.com/GER
Abstract
Background: Protein synthesis is inhibited by oxidative stress. Among the possible causes of this inhibition are the modifications of elongation factor 2 (eEF-2), the protein that catalyzes the translocation of the ribosome through mRNA. eEF-2 is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide (CH). Objective: The purpose of this study was to determine whether the antioxidant Trolox prevents the effect of CH on the levels of hepatic eEF-2. Methods: The effect was determined in liver homogenates treated with both compounds. Lipid peroxides and carbonyl content were also measured. Results: The results show that Trolox at certain doses prevents the decrease in the level of eEF-2 caused by CH. Conclusion: Under oxidative stress circumstances, vitamin E can prevent the effect of oxidations on relevant biological processes such as protein synthesis.
© 2007 S. Karger AG, Basel
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Article / Publication Details
First-Page Preview
Received: January 17, 2007
Accepted: February 20, 2007
Published online: May 16, 2007
Issue release date: August 2007
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 0304-324X (Print)
eISSN: 1423-0003 (Online)
For additional information: https://www.karger.com/GER
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