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GEISHA: an in situ hybridization gene expression resource for the chicken embryo

Darnell D.K.a · Kaur S.a · Stanislaw S.a · Davey S.a · Konieczka J.H.b · Yatskievych T.A.a · Antin P.B.a, b

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aDepartment ofCell Biology and Anatomy, and bDepartment of Molecular and Cellular Biology, University of Arizona, Tucson, AZ (USA)

Related Articles for ""

Cytogenet Genome Res 117:30–35 (2007)

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Article / Publication Details

First-Page Preview
Abstract of Resources

Published online: August 02, 2007
Issue release date: July 2007

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

An important and ongoing focus of biomedical and agricultural avian research is to understand gene function, which for a significant fraction of genes remains unknown. A first step is to determine when and where genes are expressed during development and in the adult. Whole mount in situ hybridization gives precise spatial and temporal resolution of gene expression throughout an embryo, and a comprehensive analysis and centralized repository of in situ hybridization information would provide a valuable research tool. The GEISHA project (gallus expression in situ hybridization analysis) was initiated to explore the utility of using high-throughput in situ hybridization as a means for gene discovery and annotation in chicken embryos, and to provide a unified repository for in situ hybridization information. This report describes the design and implementation of a new GEISHA database and user interface (www.geisha.arizona.edu), and illustrates its utility for researchers in the biomedical and poultry science communities. Results obtained from a high throughput screen of microRNA expression in chicken embryos are also presented.

© 2007 S. Karger AG, Basel


References

  1. Alvarez-Garcia I, Miska EA: MicroRNA functions in animal development and human disease. Development 132:4653–4662 (2005).
  2. Ambros VA: A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell 57:49–57 (1989).
  3. Bell GW, Yatskievych TA, Antin PB: GEISHA, A whole-mount in situ hybridization gene expression screen in chicken embryos. Dev Dyn 229:677–687 (2004).
  4. Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, et al: A comprehensive collection of chicken cDNAs. Curr Biol 12:1965–1969 (2002).
  5. Brennecke J, Hipfner DR, Stark A, Russel RB, Cohen SM: Bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25–36 (2003).
  6. Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, et al: Human micro RNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA 101:2999–3004 (2004).
  7. Croce CM, Calin GA: miRNAs, cancer, and stem cell division. Cell 122:6–7 (2005).
  8. Darnell DK, Kaur S, Stanislaw S, Konieczka JH, Yatskievych TA, Antin PB: MicroRNA expression during chicken embryo development. Dev Dyn 235:3156–3165 (2006).
  9. Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfus G: Numerous microRNPs in neuronal cell containing novel microRNAs. RNA 9:180–186 (2003).
  10. Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, et al: MicroRNAs regulate brain morphogenesis in zebrafish. Science 308:833–838 (2005).
  11. Hamburger V, Hamilton HL: A series of normal stages in the development of the chick embryo. J Morphol 88:49–92 (1951).
    External Resources
  12. Hammond SM: MicroRNAs as oncogenes. Curr Opin Genet Dev 16:4–9 (2006).
  13. Hatfield SD, Scherbata HR, Fischer KA, Nakahara K, Carthew RW, Ruohola-Baker H: Stem cell division is regulated by the microRNA pathway. Nature 435:974–978 (2005).
  14. Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S, Plasterk RHA: In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods 3:27–29 (2006).
  15. Koshkin AA, Singh SK, Nielsen P, Rajwanshi VK, Kumar R, et al: LNA (Locked Nucleic Acids): synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine, and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition. Tetrahedron 54:3607–3630 (1998).
  16. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, et al: The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403:901–906 (2000).
  17. Slater GS, Birney E: Automated generation of heuristics for biological sequence comparison. Bmc Bioinformatics 6:31 (2005).
  18. Weinholds E, Kloosterman WP, Miska E, Alvarez-Saavedra E, Berezikov E, et al: MicroRNA expression in zebrafish embryonic development. Science 309:310–311 (2005).

Article / Publication Details

First-Page Preview
Abstract of Resources

Published online: August 02, 2007
Issue release date: July 2007

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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