Complementary Medicine Research
Originalarbeit · Original Article
Supportive Evidence for the Anticancerous Potential of Alternative Medicine against Hepatocarcinogenesis in MicePathak S. · Bhattacharjee N. · Das J.K. · Choudhury S.C. · Karmakar S.R. · Banerjee P. · Paul S. · Banerjee A. · Khuda-Bukhsh A.Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, West Bengal, India
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Article / Publication Details
Published online: June 22, 2007
Issue release date: June 2007
Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0
ISSN: 2504-2092 (Print)
eISSN: 2504-2106 (Online)
For additional information: https://www.karger.com/CMR
Abstract
The present study examines if Lycopodium 200 (Lyco-200) has demonstrable anti-cancer activities in mice which are chronically fed carcinogens, p-dimethylaminoazobenzene (p-DAB) and phenobarbital (PB) to induce liver cancer. Materials and Methods: Mice in 5 different groups were chronically fed for varying periods of time: group I: normal diet; group II: normal diet + alcohol 200); group III: p-DAB + PB; group IV: p-DAB + PB + alcohol 200 (vehicle of Lyco-200 being ethyl alcohol); group V: p-DAB + PB + Lyco-200. They were sacrificed at day 7, 15, 30, 60, 90 or 120, and the following parameters were assessed: cytogenetic endpoints like chromosome aberrations, micronuclei, mitotic index and sperm-head anomaly; toxicity biomarkers like acid and alkaline phosphatases, alanine and aspartate amino transferase, glutathione reductase, succinate dehydrogenase and catalase activities, lipid peroxidation and reduced glutathione content. Additionally, scanning and transmission electron microscopic analyses of liver tissues were made at day 90 and 120, and immunodetection of p53 protein as well as gelatin zymography for matrix metalloproteinases in liver tissue were performed. Furthermore, studies were conducted on blood glucose, hemoglobin and cholesterol, estradiol, testosterone and cortisol, and lymphocyte and hepatic cell viabilities. Physical properties of Lyco-200 and potentized alcohol 200 were analyzed by using methods such as UV, Fourier Transform Infrared Spectroscopy (FTIR), Fluorescence Spectroscopy, 1H-NMR and 13C-NMR (Nuclear Magnetic Resonance Spectroscopy). Results: Lyco-200 reduced cytogenetic damages yielding positive modulations of all biochemical, pathological and other risk factors, cell viability and expression of p53 protein and matrix metalloproteinases as compared to controls. Conclusion: Studies on other mammals are recommended to further investigate the potential of Lyco-200 in liver cancer.
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Article / Publication Details
Published online: June 22, 2007
Issue release date: June 2007
Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0
ISSN: 2504-2092 (Print)
eISSN: 2504-2106 (Online)
For additional information: https://www.karger.com/CMR
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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