Investigation of Relaxant Effects of Propofol on Sheep Sphincter of OddiBagcivan I.a · Gursoy S.b · Yildirim M.K.a · Kaya Temiz T.a · Yildirim S.a · Yilmaz A.c · Turan M.d
Departments of aPharmacology, bAnesthesiology, cInternal Medicine, Cumhuriyet University School of Medicine, Sivas, and dGerman Hospital (Universal Hospitals Group), Department of General Surgery, Istanbul, Turkey
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Article / Publication Details
Background/Aims: Intravenous anesthetics are often used for conscious sedation in endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic sphincter of Oddi (SO) manometry. This study was designed to investigate the effects of propofol on sheep SO. Methods: SO rings were mounted in a tissue bath and tested for changes in isometric tension in response to propofol (10–8–10–4M) in the presence or absence of L-NAME (3 × 10–5M), a non-specific inhibitor of nitric oxide (NO) synthase; indomethacin (10–5M), an inhibitor of cyclooxygenase; glibenclamide (10–5M), an inhibitor of ATP-sensitive potassium channels; tetraethylammonium (3 × 10–4M), inhibitors of calcium-activated potassium channels; 4-aminopyridine (10–3M), a voltage-dependent potassium channel blocker. Furthermore, we investigated the Ca2+ antagonist feature of propofol in precontracted SO rings by CaCl2. Results: Carbachol (10–9–10–5M) induced concentration-dependent contraction responses in the SO rings. Propofol (10–8–10–4M) produced concentration-dependent relaxation on isolated SO rings precontracted by carbachol (10–6M). Preincubation of SO rings by L-NAME (3 × 10–5M), indomethacin (10–5M), glibenclamide (10–5M), and 4-aminopyridine (10–3M) did not produce a significant alteration on propofol-induced relaxation responses (p > 0.05), while preincubation by tetraethylammonium (3 × 10–4M) significantly decreased the propofol-induced relaxation responses (p < 0.05). Propofol (10–8–10–4M) induced concentration-dependently relaxations in precontracted isolated SO rings by CaCl2. Conclusion: The results suggest that propofol induced concentration-dependent relaxations in precontracted isolated SO rings. These relaxations are independent from NO, cyclooxygenase metabolites, and opened ATP-sensitive and voltage-dependent potassium channels. Opened Ca2+-sensitive K+ channels and inhibited L-type Ca2+ channels existing in smooth muscle by propofol can contribute to these relaxations. Propofol can be beneficial as alternative drugs for obtaining selective relaxation during SO manometry after controlled clinical studies.
© 2007 S. Karger AG, Basel and IAP
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.