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Original Paper

Lactate and Glycerol Released to the Intestinal Lumen Reflect Mucosal Injury and Permeability Changes Caused by Strangulation Obstruction

Juel I.S.a, c · Solligård E.b, d · Skogvoll E.b, e · Aadahl P.b, d · Grønbech J.E.a, c

Author affiliations

Departments of aSurgery and bAnaesthesiology and Intensive Care, St. Olav University Hospital, Departments of cCancer Research and Molecular Medicine, and dCirculation and Imaging, and eUnit for Applied Clinical Research, Norwegian University of Science and Technology, Trondheim, Norway

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Eur Surg Res 2007;39:340–349

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 14, 2007
Accepted: May 07, 2007
Published online: July 06, 2007
Issue release date: October 2007

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 4

ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)

For additional information: https://www.karger.com/ESR

Abstract

Background: The present study evaluates whether microdialysis of glycerol and lactate reflects mucosal injury and permeability changes after strangulation obstruction of the pig small intestine. Methods: Strangulation obstruction was induced by tightening a rubber band around a small bowel loop until its venous pressure increased to a level just below diastolic aortic pressure (partial strangulation), or further until cessation of flow in the main feeding artery (total strangulation). Mucosal injury and permeability of marker molecules from blood to lumen and vice versa was compared to release of glycerol and lactate to the intestinal lumen. Results: Mucosal injury, hyperpermeability, and release of glycerol were more pronounced after total than after partial strangulation. In animals with partial strangulation there was a complete restitution of the surface epithelium, and luminal glycerol and lumen-to-blood permeability of polyethylene glycol 4000 remained low. Such animals showed a sustained elevation of lactate and blood-to-lumen permeability of fluorescein isothiocyanate dextran after 2 h of partial strangulation, but a decline to baseline levels of these parameters in animals with 1 h partial strangulation. Conclusion: Microdialysis of lactate and glycerol in the intestinal lumen may be used to assess structural and functional changes of the intestinal mucosa after strangulation obstruction.

© 2007 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 14, 2007
Accepted: May 07, 2007
Published online: July 06, 2007
Issue release date: October 2007

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 4

ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)

For additional information: https://www.karger.com/ESR


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