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Original Paper

Free Access

Salivary Glucosyltransferase B as a Possible Marker for Caries Activity

Vacca Smith A.M.a · Scott-Anne K.M.b · Whelehan M.T.c · Berkowitz R.J.c · Feng C.d · Bowen W.H.b

Author affiliations

aDepartment of Microbiology and Immunology, bCenter for Oral Biology, cEastman Department of Dentistry and dDepartment of Biostatistics and Computational Biology, University of Rochester, Rochester, N.Y., USA

Corresponding Author

William H. Bowen, BDS, PhD

University of Rochester, Center for Oral Biology

601 Elmwood Avenue, Box 611

Rochester, NY 14642 (USA)

Tel. +1 585 275 0772, Fax +1 585 276 0190, E-Mail William_Bowen@urmc.rochester.edu

Related Articles for ""

Caries Res 2007;41:445–450

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Bacteria-derived glucosyltransferases (Gtf) (EC, through synthesizing glucan polymers from sucrose and starch hydrolysates, play an essential role in the etiology and pathogenesis of caries. We attempted to correlate the levels of Gtf in whole saliva with the prevalence of carious lesions in young children. We examined saliva from children who were either free of overt carious lesions, or had severe early childhood caries (mean dmfs = 18.72 ± 9.0 SD), for Gtf by direct enzyme assay. The levels of GtfB, GtfC and GtfD from Streptococcus mutans in the saliva using monoclonal/specific antibodies in an enzyme-linked immunosorbent assay were determined. Multiple logistic regression analyses with model selection showed that GtfB levels correlated with dmfs values of the subjects (p = 0.006). There was no correlation between total Gtf activity as measured by direct enzyme assay and dmfs values. There was a strong correlation between mutans streptococci populations in saliva and caries activity. Collectively, these data show that GtfB levels in saliva correlate strongly with presence of clinical caries and with number of carious lesions in young children. It is also possible to measure different Gtfs, separately, in whole saliva. These observations may have important clinical implications, may lead to development of a chair side caries activity test and support the importance of GtfB in the pathogenesis of dental caries.

© 2007 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: December 08, 2006
Accepted: April 11, 2007
Published online: September 07, 2007
Issue release date: November 2007

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 2

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

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