Clinical and Laboratory Investigations
Gene Expression Profiling of Melanocytes following Q-Switched Ruby Laser IrradiationHafner C.a · Stempfl T.b · Bäumler W.a · Hohenleutner U.a · Landthaler M.a · Vogt T.a
aDepartment of Dermatology and bCenter of Excellence for Fluorescent Bioanalytics, University of Regensburg, Regensburg, Germany
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Article / Publication Details
Background: The Q-switched Ruby laser (QSRL) is used for the treatment of pigmented lesions. The influence of QSRL treatment on gene expression of nontransformed primary melanocytes has not been addressed in vitro. Objective: We investigated the gene expression profile of melanocytes following QSRL irradiation. Methods: Primary melanocytes were irradiated with the QSRL (694 nm). Early and late transcriptional effects were analyzed using the Affymetrix gene array platform. Results: Laser irradiation of melanocytes had minor effects on mRNA expression. We found only 31 out of 14,500 genes which were at least twofold up- or downregulated. The differential expression of heme oxygenase 1 and galanin in QSRL-treated melanocytes was additionally confirmed by real-time RT-PCR. Analysis of a selection of 36 genes which are known to be associated with malignant melanoma development and progression revealed no significantly aberrant expression in the QSRL-treated melanocytes. Conclusion: Our study shows that QSRL treatment of primary melanocytes in vitro does not cause major alterations of global gene expression and particularly of genes associated with malignant melanoma. However, since QSRL treatment may have different effects on gene expression of melanocytic cells in vivo, further studies are required to evaluate QSRL treatment of (nevo-) melanocytic lesions.
© 2008 S. Karger AG, Basel
- Michel S, Hohenleutner U, Baumler W, Landthaler M: Q-switched ruby laser in dermatologic therapy. Use and indications (in German). Hautarzt 1997;48:462–470.
- Nelson JS, Applebaum J: Treatment of superficial cutaneous pigmented lesions by melanin-specific selective photothermolysis using the Q-switched ruby laser. Ann Plast Surg 1992;29:231–237.
- Raulin C, Schonermark MP, Greve B, Werner S: Q-switched ruby laser treatment of tattoos and benign pigmented skin lesions: a critical review. Ann Plast Surg 1998;41:555–565.
- Kopera D, Hohenleutner U, Landthaler M: Quality-switched ruby laser treatment of solar lentigines and Becker’s nevus: a histopathological and immunohistochemical study. Dermatology 1997;194:338–343.
- Anderson RR, Parrish JA: Selective photothermolysis: precise microsurgery by selective absorption of pulsed radiation. Science 1983;220:524–527.
- Anderson RR, Margolis RJ, Watenabe S, Flotte T, Hruza GJ, Dover JS: Selective photothermolysis of cutaneous pigmentation by Q-switched Nd: YAG laser pulses at 1064, 532, and 355 nm. J Invest Dermatol 1989;93:28–32.
- Duke D, Byers HR, Sober AJ, Anderson RR, Grevelink JM: Treatment of benign and atypical nevi with the normal-mode ruby laser and the Q-switched ruby laser: clinical improvement but failure to completely eliminate nevomelanocytes. Arch Dermatol 1999;135:290–296.
- Gottschaller C, Hohenleutner U, Landthaler M: Metastasis of a malignant melanoma 2 years after carbon dioxide laser treatment of a pigmented lesion: case report and review of the literature. Acta Derm Venereol 2006;86:44–47.
- Greve B, Raulin C: Professional errors caused by lasers and intense pulsed light technology in dermatology and aesthetic medicine: preventive strategies and case studies. Dermatol Surg 2002;28:156–161.
- Lee PK, Rosenberg CN, Tsao H, Sober AJ: Failure of Q-switched ruby laser to eradicate atypical-appearing solar lentigo: report of two cases. J Am Acad Dermatol 1998;38:314–317.
- Dummer R: About moles, melanomas, and lasers: the dermatologist’s schizophrenic attitude toward pigmented lesions. Arch Dermatol 2003;139:1405–1406.
- Chan HH, Xiang L, Leung JC, Tsang KW, Lai KN: In vitro study examining the effect of sub-lethal QS 755 nm lasers on the expression of p16INK4a on melanoma cell lines. Lasers Surg Med 2003;32:88–93.
- Zhu NW, Kenealy J, Burd A, et al: Sub-lethal effects of exposing the human melanoma cell line SKmel-23 to 532 nm laser light. Int J Cancer 1997;72:1104–1112.
- Zhu NW, Perks CM, Burd AR, Holly JM: Changes in the levels of integrin and focal adhesion kinase (FAK) in human melanoma cells following 532 nm laser treatment. Int J Cancer 1999;82:353–358.
- Hafner C, Schmitz G, Meyer S, et al: Differential gene expression of Eph receptors and ephrins in benign human tissues and cancers. Clin Chem 2004;50:490–499.
- Alonso SR, Ortiz P, Pollan M, et al: Progression in cutaneous malignant melanoma is associated with distinct expression profiles: a tissue microarray-based study. Am J Pathol 2004;164:193–203.
Fensterle J: A trip through the signaling pathways of melanoma (in German). J Dtsch Dermatol Ges 2006;4:205–217.
- van Leeuwen RL, Dekker SK, Byers HR, Vermeer BJ, Grevelink JM: Modulation of alpha 4 beta 1 and alpha 5 beta 1 integrin expression: heterogeneous effects of Q-switched ruby, Nd:YAG, and alexandrite lasers on melanoma cells in vitro. Lasers Surg Med 1996;18:63–71.
- Sohn S, Kim S, Kang WH: Recurrent pigmented macules after q-switched alexandrite laser treatment of congenital melanocytic nevus. Dermatol Surg 2004;30:898–907; discussion 907.
- Vile GF, Basu-Modak S, Waltner C, Tyrrell RM: Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts. Proc Natl Acad Sci USA 1994;91:2607–2610.
- Tyrrell RM: Solar ultraviolet A radiation: an oxidizing skin carcinogen that activates heme oxygenase-1. Antioxid Redox Signal 2004;6:835–840.
Okamoto I, Krogler J, Endler G, et al: A microsatellite polymorphism in the heme oxygenase-1 gene promoter is associated with risk for melanoma. Int J Cancer 2006.
- Kofler B, Berger A, Santic R, et al: Expression of neuropeptide galanin and galanin receptors in human skin. J Invest Dermatol 2004;122:1050–1053.
- Berger A, Lang R, Moritz K, et al: Galanin receptor subtype GalR2 mediates apoptosis in SH-SY5Y neuroblastoma cells. Endocrinology 2004;145:500–507.
- Vogt TM, Welsh J, Stolz W, et al: RNA fingerprinting displays UVB-specific disruption of transcriptional control in human melanocytes. Cancer Res 1997;57:3554–3561.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.