Developmental Neuroscience

Original Paper

Localization of Alkaline Phosphatase Activity in Endothelia of Developing and Mature Mouse Blood-Brain Barrier

Vorbrodt A.W. · Lossinsky A.S. · Wisniewski H.M.

Author affiliations

Department of Pathological Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, Staten Island, N.Y., USA

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Dev Neurosci 1986;8:1–13

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 30, 1986
Issue release date: 1986

Number of Print Pages: 13
Number of Figures: 0
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Abstract

The relationship between alkaline phosphatase (AP) activity and maturation of blood-brain barrier (BBB) in mouse brain was studied ultracytochemically. The permeability of micro-blood vessels (MBVs) to intravenously injected horseradish peroxidase was considered to be a criterion of BBB maturation, which occurs between the 12th and 24th day of mouse life. This process coincides with the appearance of cytochemically detectable AP activity in luminal plasma membrane (PM) of the endothelial cell (EC) of capillaries or in both luminal and abluminal PM in arterioles. No reaction appears in ECs of venules. These observations indicate that the development of BBB function is accompanied by the formation of enzymatic barrier in MBVs endothelia. AP constitutes one of the components of this barrier. Comparative study indicates that in various MBVs of non-BBB type, except in skeletal muscle, no AP activity is detected in similar incubation conditions.

© 1986 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 30, 1986
Issue release date: 1986

Number of Print Pages: 13
Number of Figures: 0
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE


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