Developmental Neuroscience

Original Papers

A Quantitative Ultrastructural Study of the Effects of Phenylacetate on Synaptic Organization in the Developing Rat Cerebral Cortex

Oorschot D.E. · Jones D.G.

Author affiliations

Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Australia, and Department of Anatomy, University of Otago, Dunedin, New Zealand

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Dev Neurosci 1983–84;6:45–57

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Article / Publication Details

First-Page Preview
Abstract of Original Papers

Received: August 29, 1983
Accepted: September 16, 1983
Published online: June 29, 2009
Issue release date: 1984

Number of Print Pages: 13
Number of Figures: 0
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Abstract

Male rats were injected with either phenylacetate (PA; a phenylalanine metabolite) or saline (control). Osmicated tissue from the parietal cortex was analyzed qualitatively and quantitatively, attention being focused on possible synaptic changes in the molecular layer. PA-treated rats differed from controls in the following ways: a 20% decrease in body weight at 21 days, and a reduction in brain weight at 10 and 21 days; the presence of 'atypical' profiles (possibly derived from axonal growth cones) in the neuropil; a lag in the accretion of synapses per 100 μm2 of tissue, and of vesicles per μm2of presynaptic terminal; the presence of a 'crossover' in the developmental trend for several synaptic organizational parameters between days 15 and 21; an elevation in synaptic density at day 21; an increased proportion of negatively curved synapses at 21 days; a significant difference in the preponderance of axodendritic synapses over days 7–21. This suggests that the metabolism of some neurons in the PA brain is initially different from normal, with a lag in synaptic development being evident. The increase in synaptic density and the relative preponderance of other synaptic parameters at 21 days signifies that an initial lag in development may alter the developmental scheme. This may be coupled with changes in synaptic efficacy and connectivity.

© 1983 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Papers

Received: August 29, 1983
Accepted: September 16, 1983
Published online: June 29, 2009
Issue release date: 1984

Number of Print Pages: 13
Number of Figures: 0
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE


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