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Laboratory Investigation

Epigenetic and Genetic Alterations of the EDNRB Gene in Nasopharyngeal Carcinoma

Zhou L.a, c · Feng X.a · Shan W.a · Zhou W.a · Liu W.a · Wang L.a · Zhu B.a · Yi H.a, b · Yao K.a, d · Ren C.a

Author affiliations

aCancer Research Institute, Xiang-Ya School of Medicine, Central South University and bInstitute of Clinical Medicine, Hunan Province People’s Hospital, Changsha, cPeking University Shenzhen Hospital, Medical Center of Peking University and Hongkong Science and Technology University, Shenzhen, and dCancer Research Institute, Southern Medical University, Guangzhou, PR China

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Oncology 2007;72:357–363

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Article / Publication Details

First-Page Preview
Abstract of Laboratory Investigation

Received: April 20, 2007
Accepted: August 05, 2007
Published online: January 14, 2008
Issue release date: February 2008

Number of Print Pages: 7
Number of Figures: 5
Number of Tables: 1

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL

Abstract

Background: Loss of heterozygosity (LOH) at 13q22 is a common event in nasopharyngeal carcinoma (NPC). EDNRB gene located at 13q22 has been demonstrated to be hypermethylated in some kinds of tumors. In the current study, we focused on the epigenetic and genetic alterations of EDNRB in NPC. Methods: The mRNA expression of EDNRB was detected by semiquantitative RT-PCR and real-time quantitative PCR in 49 NPC and 12 chronic nasopharyngitis biopsies. The methylation and LOH status of EDNRB were examined by methylation-specific polymerase chain reaction, microsatellite PCR and sequencing. We also examined the mRNA expression of EDNRB in four NPC cell lines after 5-aza-2′-deoxycytidine treatment. Results:EDNRB was downregulated in primary NPC tissues and NPC cell lines, and a relatively higher methylation level of EDNRB was found in NPC biopsies (84%) compared to that in chronic nasopharyngitis biopsies (42%). Treatment of NPC cell lines with 5-aza-2′-deoxycytidine activated EDNRB expression. LOH of EDNRB gene was also found at two microsatellite sites with ratios of 6.25 and 16.67% in NPC. Conclusion: Our results suggested that EDNRB expression may be affected by aberrant promoter methylation and gene deletion and may play a role in the development of NPC.

© 2008 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Laboratory Investigation

Received: April 20, 2007
Accepted: August 05, 2007
Published online: January 14, 2008
Issue release date: February 2008

Number of Print Pages: 7
Number of Figures: 5
Number of Tables: 1

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL


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