Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Original Paper

Purification and Characterization of the Major Oak Pollen Allergen Que a 1 for Component-Resolved Diagnostics Using ImmunoCAP®

Movérare R.a, b · Everberg H.a · Carlsson R.a · Holtz A.a · Thunberg R.a · Olsson P.a · Brostedt P.a · Högbom E.a

Author affiliations

aPhadia AB and bDepartment of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, Uppsala, Sweden

Related Articles for ""

Int Arch Allergy Immunol 2008;146:203–211

Do you have an account?

Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.


Save over 20% compared to the individual article price.
Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00


Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 16, 2007
Accepted: October 18, 2007
Published online: February 11, 2008
Issue release date: June 2008

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: The aim of this study was to purify the major oak pollen allergen, Que a 1, to perform biochemical and immunological characterization of the allergen and to develop an experimental native (n) Que a 1 ImmunoCAP®. Methods: Que a 1 was purified from oak pollen extract using affinity chromatography and characterized by SDS-PAGE, two-dimensional (2D) PAGE, mass spectrometry (MS), N-terminal sequencing and specific IgE inhibition on ImmunoCAP. Samples from 16 subjects sensitized to oak pollen were analyzed by ImmunoCAP for IgE reactivity to nQue a 1, and recombinant (r)Bet v 1 and 2 (profilin). They were also studied in IgE immunoblotting. Results: The purity of nQue a 1 was >95%, since a single band was observed on silver-stained SDS-PAGE. The identity was verified by MS analysis, and 2D-PAGE revealed several isoforms. The obtained N-terminal sequence of 50-amino-acid residues from nQue a 1 showed a 58–74% sequence identity with other pathogenesis-related class 10 allergens. Specific IgE inhibition verified a preserved immunoreactivity (70–92% inhibition). All subjects were sensitized to Que a 1 and Bet v 1, and two to profilin. The IgE antibody levels to nQue a 1 were generally lower than to rBet v 1. The obtained results correlated well with IgE immunoblotting. Conclusions: We present a highly purified and extensively characterized preparation of nQue a 1. Que a 1 seems to be an allergen of equal importance in oak pollen as Bet v 1 in birch pollen. An nQue a 1 ImmunoCAP will be useful in component-resolved diagnostics.

© 2008 S. Karger AG, Basel


References

  1. Burney P, Malmberg E, Chinn S, Jarvis D, Luczynska C, Lai E: The distribution of total and specific serum IgE in the European Community Respiratory Health Survey. J Allergy Clin Immunol 1997;99:314–322.
  2. Breiteneder H, Pettenburger K, Bito A, Valenta R, Kraft D, Rumpold H, Scheiner O, Breitenbach M: The gene coding for the major birch pollen allergen Bet v 1, is highly homologous to a pea disease resistance response gene. EMBO J 1989;8:1935–1938.
  3. Schenk MF, Gilissen LJ, Esselink GD, Smulders MJ: Seven different genes encode a diverse mixture of isoforms of Bet v 1, the major birch pollen allergen. BMC Genomics 2006;7:168.
  4. Rohac M, Birkner T, Reimitzer I, Bohle B, Steiner R, Breitenbach M, Kraft D, Scheiner O, Gabl F, Rumpold H: The immunological relationship of epitopes on major tree pollen allergens. Mol Immunol 1991;28:897–906.
  5. Swoboda I, Jilek A, Ferreira F, Engel E, Hoffmann-Sommergruber K, Scheiner O, Kraft D, Breiteneder H, Pittenauer E, Schmid E, Vicente O, Heberle-Bors E, Ahorn H, Breitenbach M: Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J Biol Chem 1995;270:2607–2613.
  6. Jarolim E, Rumpold H, Endler AT, Ebner H, Breitenbach M, Scheiner O, Kraft D: IgE and IgG antibodies of patients with allergy to birch pollen as tools to define the allergen profile of Betula verrucosa. Allergy 1989;44:385–395.
  7. Moverare R, Westritschnig K, Svensson M, Hayek B, Bende M, Pauli G, Sorva R, Haahtela T, Valenta R, Elfman L: Different IgE reactivity profiles in birch pollen-sensitive patients from six European populations revealed by recombinant allergens. An inprint of local sensitization. Int Arch Allergy Immunol 2002;128:325–335.
  8. Kazemi-Shirazi L, Niederberger V, Linhart B, Lidholm J, Kraft D, Valenta R: Recombinant marker allergens: diagnostic gatekeepers for the treatment of allergy. Int Arch Allergy Immunol 2002;127:259–268.
  9. Luttkopf D, Muller U, Skov PS, Ballmer-Weber BK, Wuthrich B, Skamstrup Hansen K, Poulsen LK, Kastner M, Haustein D, Vieths S: Comparison of four variants of a major allergen in hazelnut (Corylus avellana) Cor a 1.04 with the major hazel pollen allergen Cor a 1.01. Mol Immunol 2002;38:515–525.
  10. Ross AM, Corden JM, Fleming DM: The role of oak pollen in hay fever consultations in general practice and the factors influencing patients’ decisions to consult. Br J Gen Pract 1996;46:451–455.
  11. Lin RY, Clauss AE, Bennett ES: Hypersensitivity to common tree pollens in New York City patients. Allergy Asthma Proc 2002;23:253–258.
  12. Subiza J, Jerez M, Jimenez JA, Narganes MJ, Cabrera M, Varela S, Subiza E: Allergenic pollen and pollinosis in Madrid. J Allergy Clin Immunol 1995;96:15–23.
  13. Eriksson NE, Wihl JA, Arrendal H, Strandhede SO: Tree pollen allergy. II. Sensitization to various tree pollen allergens in Sweden. A multi-centre study. Allergy 1984;39:610–617.
  14. Niederberger V, Pauli G, Gronlund H, Froschl R, Rumpold H, Kraft D, Valenta R, Spitzauer S: Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen: a quantitative IgE inhibition study with sera from different populations. J Allergy Clin Immunol 1998;102:579–591.
  15. Ipsen H, Hansen OC: The NH2-terminal amino acid sequence of the immunochemically partial identical major allergens of alder (Alnus glutinosa) Aln g I, birch (Betula verrucosa) Bet v I, hornbeam (Carpinus betulus) Car b I and oak (Quercus alba) Que a I pollens. Mol Immunol 1991;28:1279–1288.
  16. Jarolim E, Tejkl M, Rohac M, Schlerka G, Scheiner O, Kraft D, Breitenbach M, Rumpold H: Monoclonal antibodies against birch pollen allergens: characterization by immunoblotting and use for single-step affinity purification of the major allergen Bet v I. Int Arch Allergy Appl Immunol 1989;90:54–60.
  17. Laffer S, Vangelista L, Steinberger P, Kraft D, Pastore A, Valenta R: Molecular characterization of Bip 1, a monoclonal antibody that modulates IgE binding to birch pollen allergen, Bet v 1. J Immunol 1996;157:4953–4962.
  18. Blum H, Beier H, Gross HJ: Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987;8:93–99.
  19. Smirnov IP, Zhu X, Taylor T, Huang Y, Ross P, Papayanopoulos IA, Martin SA, Pappin DJ: Suppression of alpha-cyano-4-hydroxycinnamic acid matrix clusters and reduction of chemical noise in MALDI-TOF mass spectrometry. Anal Chem 2004;76:2958–2965.
  20. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 1990;215:403–410.
  21. Notredame C, Higgins DG, Heringa J: T-Coffee: a novel method for fast and accurate multiple sequence alignment. J Mol Biol 2000;302:205–217.
  22. Wensing M, Akkerdaas JH, van Leeuwen WA, Stapel SO, Bruijnzeel-Koomen CA, Aalberse RC, Bast BJ, Knulst AC, van Ree R: IgE to Bet v 1 and profilin: cross-reactivity patterns and clinical relevance. J Allergy Clin Immunol 2002;110:435–442.
  23. Akkerdaas JH, van Ree R, Aalbers M, Stapel SO, Aalberse RC: Multiplicity of cross-reactive epitopes on Bet v I as detected with monoclonal antibodies and human IgE. Allergy 1995;50:215–220.
  24. Kos T, Hoffmann-Sommergruber K, Ferreira F, Hirschwehr R, Ahorn H, Horak F, Jager S, Sperr W, Kraft D, Scheiner O: Purification, characterization and N-terminal amino acid sequence of a new major allergen from European chestnut pollen – Cas s 1. Biochem Biophys Res Commun 1993;196:1086–1092.
  25. van Ree R, van Leeuwen WA, Akkerdaas JH, Aalberse RC: How far can we simplify in vitro diagnostics for Fagales tree pollen allergy? A study with three whole pollen extracts and purified natural and recombinant allergens. Clin Exp Allergy 1999;29:848–855.
  26. DeWitt AM, Andersson K, Peltre G, Lidholm J: Cloning, expression and immunological characterization of full-length timothy grass pollen allergen Phl p 4, a berberine bridge enzyme-like protein with homology to celery allergen Api g 5. Clin Exp Allergy 2006;36:77–86.
  27. Ferreira F, Hirtenlehner K, Jilek A, Godnik-Cvar J, Breiteneder H, Grimm R, Hoffmann-Sommergruber K, Scheiner O, Kraft D, Breitenbach M, Rheinberger HJ, Ebner C: Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for immunotherapy. J Exp Med 1996;183:599–609.
  28. Chang ZN, Liu CC, Tam MF, Peng HJ, Tsai JJ, Han SH: Characterization of the isoforms of the group I allergen of Cynodon dactylon. J Allergy Clin Immunol 1995;95:1206–1214.
  29. Altmann F: The role of protein glycosylation in allergy. Int Arch Allergy Immunol 2007;142:99–115.
  30. Valenta R, Duchene M, Vrtala S, Birkner T, Ebner C, Hirschwehr R, Breitenbach M, Rumpold H, Scheiner O, Kraft D: Recombinant allergens for immunoblot diagnosis of tree-pollen allergy. J Allergy Clin Immunol 1991;88:889–894.
  31. Eriksson NE: Allergy to pollen from different deciduous trees in Sweden. An investigation with skin tests, provocation tests and the radioallergosorbent test (RAST) in springtime hay fever patients. Allergy 1978;33:299–309.
  32. Dohsaka Y, Maguchi S, Takagi S, Nagahashi T, Fukuda S, Inuyama Y: Effect of oak pollen on patients with birch pollinosis (in Japanese). Nippon Jibiinkoka Gakkai Kaiho 1995;98:357–361.
  33. Loria RC, Wilson P, Wedner HJ: Identification of potential allergens in white oak (Quercus alba) pollen by immunoblotting. J Allergy Clin Immunol 1989;84:9–18.
  34. Moverare R: Immunological mechanisms of specific immunotherapy with pollen vaccines: implications for diagnostics and the development of improved vaccination strategies. Expert Rev Vaccines 2003;2:85–97.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 16, 2007
Accepted: October 18, 2007
Published online: February 11, 2008
Issue release date: June 2008

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.