Neuroendocrinology
Original Paper
Immunocytochemical Localization of Luteinizing Hormone-Releasing Hormone in Male and Female Rat BrainsQuantitative Studies on the Effect of Gonadal Steroids
Shivers B.D. · Harlan R.E. · Morrell J.I. · Pfaff D.W.
Rockefeller University, New york, N.Y.
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Article / Publication Details
Received: February 19, 1982
Accepted: June 25, 1982
Published online: March 26, 2008
Issue release date: 1983
Number of Print Pages: 12
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
Abstract
The peroxidase-antiperoxidase immunocytochemical method was used to determine quantitatively the effects of gonadal steroids on the immunoreactive luteinizing hormone-releasing hormone (LHRH) content in the brain of male and female rats. In the male rat, gonadectomy decreased both the number of cell bodies and optical density of staining in cell bodies containing immunoreactive LHRH; decreased the percentage of area covered by LHRH fibers in the middle and caudal aspects of the median eminence (ME) but not the organum vasculosum lamina terminalis (OVLT), and decreased the number of LHRH fibers localized in the midbrain central gray (MCG). Since others have shown previously that gonadectomy increases the LHRH content of portal blood in the male rat, the results suggest that the decreased somal accumulation of LHRH and decreased LHRH content in fibers in the ME and MCG measured in the present study following loss of testicular steroids reflect increased LHRH release from the ME and MCG into the portal blood and brain, respectively.
© 1983 S. Karger AG, Basel
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Article / Publication Details
Received: February 19, 1982
Accepted: June 25, 1982
Published online: March 26, 2008
Issue release date: 1983
Number of Print Pages: 12
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
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