Neuroendocrinology
Original Paper
Cerebrospinal Fluid Levels of Acetylcholinesterase, Monoamines and Oxytocin during Labour, Parturition, Vaginocervical Stimulation, Lamb Separation and Suckling in SheepKendrick K.M.a · Keverne E.B.b · Baldwin B.A.a · Sharman D.F.aaAFRC Institute of Animal Physiology and Genetics, Babraham, and b Department of Anatomy, University of Cambridge, UK
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Article / Publication Details
Received: December 16, 1985
Accepted: April 09, 1986
Published online: April 01, 2008
Issue release date: 1986
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
Abstract
Acetylcholinesterase (AChE) activity, and concentrations of monoamines, monoamine metabolites and oxytocin (OT) were measured in the cerebrospinal fluid (CSF) of sheep during late pregnancy, labour, parturition, vaginocervical stimulation, lamb separation and suckling. Concentrations of AChE, 4-hydroxy-3 methoxyphenylethan-1,2-diol (MHPG) and OT were significantly elevated during labour and parturition. OT levels were also significantly raised in cycling ewes given vaginocervical stimulation. Separation of the ewes from their lambs (0.5–2 h) caused significant increases in AChE and MHPG, but not in OT. During suckling, following reunion of the ewes and lambs, concentrations of AChE and OT were significantly raised. The dopamine metabolites 3,4-dihydroxyphenylacetic acid and homovanillic acid were significantly lower in CSF during late pregnancy than during parturition and post-partum. Intravenous injections of OT which produced high circulating levels of this hormone in plasma produced significant, but very small, increases in concentrations of OT in the CSF. Our results show that in the sheep, labour, parturition, suckling and vaginocervical stimulation provoke a release of OT in the brain similar to that in the peripheral circulation. Changes in CSF levels of AChE and MHPG during labour, parturition and lamb separation, but not during vaginocervical stimulation, may be related to stress or hypertension.
© 1986 S. Karger AG, Basel
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Article / Publication Details
Received: December 16, 1985
Accepted: April 09, 1986
Published online: April 01, 2008
Issue release date: 1986
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
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