International Archives of Allergy and Immunology

Original Paper

Induction of Apoptosis in Human Basophils by Anti-Fas Antibody Treatment in vitro

Matsumoto K.a · Maeda A.b · Bochner B.S.c · Wakiguchi H.b · Saito H.a

Author affiliations

aDepartment of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo and bDepartment of Pediatrics, Kochi Medical School, Kochi, Japan; cDepartment of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, Md., USA

Keywords: BasophilApoptosisFasSurvival

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Int Arch Allergy Immunol 2008;146:40–46
https://doi.org/10.1159/000126060

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 27, 2008
Issue release date: May 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: Basophils are thought to play an important role in the pathogenesis of allergic inflammation; however, the factors associated with basophil death are not fully understood. Fas (CD95) is a member of the TNF receptor superfamily and is known to induce apoptosis in activated T cells, neutrophils and eosinophils. In the present study, the expression and function of Fas in human basophils were investigated in vitro. Methods: Human cultured basophils were obtained by culturing cord blood-derived CD34+ cells in the presence of 2.5 ng/ml of IL-3 for 5–6 weeks. The expression of Fas was measured using flow cytometry. Cell viability and morphological changes after the incubation of basophils with anti-Fas mAb (clone CH11, IgM) in the presence of 1 ng/ml of IL-3 were measured using the trypan blue dye exclusion test and light microscopy, respectively. Results: Human cultured basophils constitutively and significantly expressed Fas on their cell surfaces. Treatment with anti-Fas monoclonal antibody (mAb) significantly reduced basophil viability in a time- and dose-dependent manner. When basophils were incubated with 10 ng/ml of anti-Fas mAb or control for 72 h, the basophil viability was 27.3 ± 8.8% and 89.3 ± 5.2%, respectively (p < 0.01). Anti-Fas mAb-treated basophils were shrunken and exhibited condensed nuclei, consistent with apoptosis. Conclusions: Our findings indicate that human basophils express functional Fas on their cell surfaces, and signaling via Fas may regulate basophil survival in vivo.

© 2008 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 27, 2008
Issue release date: May 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

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