Neuroendocrinology
Original Paper
Interleukin-1β and Interleukin-6 Specifically Increase the Release of Prostaglandin E2 from Rat Hypothalamic Explants in vitroNavarra P.a · Pozzoli G.a · Brunetti L.a · Ragazzoni E.a · Besser M.b · Grossman A.baDepartment of Pharmacology, Catholic University Medical School, Rome, Italy; bDepartment of Endocrinology, St. Bartholomew’s Hospital, London, UK
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Article / Publication Details
Received: June 07, 1991
Accepted: October 31, 1991
Published online: April 07, 2008
Issue release date: 1992
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
Abstract
It has previously been shown that the cytokines interleukin-1β and interleukin-6 (IL-1β and IL-6) stimulate directly the release of corticotrophin-releasing-hormone-41 from the rat hypothalamus in vitro, while IL-1β can also stimulate the release of somatostatin. These effects can be antagonized by drugs which block prostaglandin (PG) synthesis. PGs are also involved in the control of hypothalamic neuropeptides by other neuron transmitters. In the present study, we have characterized the production of PGs from the rat hypothalamus in vitro, and investigated the effects of IL-1β and IL-6, as well as the neurotransmitters norepinephrine, acetylcholine and 5-hydroxytryptamine, on the acute release of PGs, using a well-validated acute hypothalamic incubation system. The rate of release of PGs [PGE2, PGF2α 6-keto-PGF1α (6KPGF1α) and thromboxane B2 (TXB2) in the medium was found to stabilize after 60 min of preincubation and thereafter remain constant, with TXB2 being the predominant species. Twenty-minute incubation in the presence of human recombinant IL-1β or IL-6, in the dose range 1-100 U/ml, had no effect on the release of PGF2α, 6KPGF1α or TXB2; however, the release of PGE2 was significantly increased by both IL-1β and IL-6. The effect of IL-1β was antagonized by both indomethacin and dexamethasone. None of the other neurotransmitters tested had any effect on the release of any of the PGs. It is concluded that the regulation of hypothalamic PG release may be investigated by means of acute rat hypothalamic explants, as has previously been shown for hypothalamic peptides and amines. Furthermore, IL-1β and IL-6 specifically stimulate PGE2 release, possibly implicating this PG in certain of the hypothalamic effects of IL-1β and IL-6.
© 1992 S. Karger AG, Basel
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Article / Publication Details
Received: June 07, 1991
Accepted: October 31, 1991
Published online: April 07, 2008
Issue release date: 1992
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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