Neuroendocrinology
Hypothalamic Adrenal Axis
Immunocytochemical Localization of Dopamine-Releasing Protein in the Rat AdrenalChoi W.S.a · Kim M.-O.a · Ramirez V.D.baDepartment of Anatomy, Gyeongsang National University, Chinju, Korea; bDepartment of Physiology and Biophysics, University of Illinois at Urbana-Champaign, Urbana, Ill., USA
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Article / Publication Details
Received: March 23, 1993
Accepted: July 09, 1993
Published online: April 08, 2008
Issue release date: 1993
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
Abstract
The mechanism by which adrenal medulla transplants to the brain of Parkinson’s patients ameliorate the symptoms of this neurodegenerative disease is unknown. It has been postulated but not proven that these transplants secrete a neurotrophic factor(s) responsible for the activation of the dopamine (DA) neurons. DA-releasing protein (DARP) was identified by immunocytochemistry in cells of the adrenal gland but not in tissues such as the liver or spleen. In the adrenal gland the immunopositive DARP cells were distributed both in the cortex as well as in the medulla. In the former, the distribution was rather uniform covering a broad area of the zona glomerulosa and fasciculata without positive cells in the zona reticularis. In the adrenal medulla the DARP signal was found in segregated cells forming clusters similar to the homotypic distribution of norepinephrine and epinephrine cells. The immunopositive cells for DARP were oval or spindle in shape and the staining was localized in the cytoplasm surrounding the nucleus. In addition, in this paper we present evidence confirming that the adrenal gland is the source of a protein that after immunopurification in nanogram amounts releases DA from corpus striatum fragments superfused in vitro.
© 1993 S. Karger AG, Basel
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Article / Publication Details
Received: March 23, 1993
Accepted: July 09, 1993
Published online: April 08, 2008
Issue release date: 1993
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)
For additional information: https://www.karger.com/NEN
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