European Surgical Research

Original Paper

Effects of Intrasplenic Injection of Hepatocytes, Hepatocyte Fragments and Hepatocyte Culture Supernatants on D-Galactosamine-Induced Liver Failure in Rats

Baumgartner D. · LaPlante-O’Neill P.M. · Sutherland D.E.R. · Najarian J.S.

Author affiliations

Department of Surgery, University of Minnesota Health Sciences Center, Minneapolis, Minn., USA

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Eur Surg Res 1983;15:129–135

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 07, 1982
Accepted: August 25, 1982
Published online: April 18, 2008
Issue release date: 1983

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)

For additional information: https://www.karger.com/ESR

Abstract

Intraperitoneal injection of 0.5 g/kg D-galactosamine results in 95% lethal acute liver failure in male Fisher 344 rats. Intrasplenic injection of viable syngeneic hepatocytes 20–28 h after poisoning improves survival in a dose-dependent fashion, 107 cells being the optimal dose with a survival rate of 47.1%. While nonviable cells and hepatocyte fragments are totally ineffective, 42.9% of rats survive after injection of 28-hour liver cell culture supernatant. It is concluded that soluble factors generated by cultured cells in vitro or intrasplenically transplanted cells improve survival either by a direct hepatotrophic effect, by stimulation of the reticuloendothelial system or by an unspecific humoral mechanism.

© 1983 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 07, 1982
Accepted: August 25, 1982
Published online: April 18, 2008
Issue release date: 1983

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)

For additional information: https://www.karger.com/ESR


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