European Surgical Research
Original Paper
Effects of Intrasplenic Injection of Hepatocytes, Hepatocyte Fragments and Hepatocyte Culture Supernatants on D-Galactosamine-Induced Liver Failure in RatsBaumgartner D. · LaPlante-O’Neill P.M. · Sutherland D.E.R. · Najarian J.S.Department of Surgery, University of Minnesota Health Sciences Center, Minneapolis, Minn., USA
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Article / Publication Details
Received: June 07, 1982
Accepted: August 25, 1982
Published online: April 18, 2008
Issue release date: 1983
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
Abstract
Intraperitoneal injection of 0.5 g/kg D-galactosamine results in 95% lethal acute liver failure in male Fisher 344 rats. Intrasplenic injection of viable syngeneic hepatocytes 20–28 h after poisoning improves survival in a dose-dependent fashion, 107 cells being the optimal dose with a survival rate of 47.1%. While nonviable cells and hepatocyte fragments are totally ineffective, 42.9% of rats survive after injection of 28-hour liver cell culture supernatant. It is concluded that soluble factors generated by cultured cells in vitro or intrasplenically transplanted cells improve survival either by a direct hepatotrophic effect, by stimulation of the reticuloendothelial system or by an unspecific humoral mechanism.
© 1983 S. Karger AG, Basel
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Article / Publication Details
Received: June 07, 1982
Accepted: August 25, 1982
Published online: April 18, 2008
Issue release date: 1983
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 0014-312X (Print)
eISSN: 1421-9921 (Online)
For additional information: https://www.karger.com/ESR
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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